Polymerase variants
US-10308918-B2 · Jun 4, 2019 · US
Exonuclease deficient polymerases
US11718836B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11718836-B2 |
| Application number | US-202117248768-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 5, 2021 |
| Priority date | Feb 29, 2016 |
| Publication date | Aug 8, 2023 |
| Grant date | Aug 8, 2023 |
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- Title
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- Abstract
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Abstract
Official abstract text for this publication.
Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.
First claim
Opening claim text (preview).
What is claimed is: 1. A DNA polymerase comprising an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 1, wherein the DNA polymerase: (A) has a lower 3′→5′ exonuclease activity than a reference DNA polymerase, wherein the reference DNA polymerase consists of SEQ ID NO: 1, (B) has the same or higher strand displacement activity than the reference DNA polymerase, and (C) has a set of substitutions relative to SEQ ID NO: 1, and wherein at least one substitution of the set of substitutions is at a position selected from the group consisting of L125K, D128H, V179Y, V179R, S211F, Y212K, I215L, T216K, Q221R, Y242G/A/L/S, Y259G/K/Q, A292K, S293G, and S294T. 2. The DNA polymerase of claim 1 , wherein the set of substitutions comprises each of T529M, S366A, A547F, and Y242G/A/L/S. 3. The DNA polymerase of claim 1 , wherein the DNA polymerase further has an extension rate that is greater than or equal to the extension rate of the reference DNA polymerase. 4. The DNA polymerase of claim 3 , wherein the extension rate is at least 1.5× greater than the extension rate of the reference DNA polymerase. 5. The DNA polymerase of claim 3 , wherein the extension rate is from 1.5× to 5× greater than the extension rate of the reference DNA polymerase.
Assignees
Inventors
Classifications
- C12N9/1252Primary
DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase · CPC title
DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase · CPC title
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Frequently asked questions
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- What does patent US11718836B2 cover?
- Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase…
- Who is the assignee on this patent?
- Roche Sequencing Solutions Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/1252. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 08 2023 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 6 related publications on this page (citations in our corpus or others sharing the same primary CPC).