Point-of-care apparatus and methods for analyte detections using electrochemical impedance or capacitance

Patent metadata

Field	Value
Publication number	US-11714083-B2
Application number	US-201816612270-A
Country	US
Kind code	B2
Filing date	May 4, 2018
Priority date	May 11, 2017
Publication date	Aug 1, 2023
Grant date	Aug 1, 2023

Abstract

The presence of analytes can be detected in the bodily fluid using Electrochemical Impedance Spectroscopy (EIS) or Electrochemical Capacitance Spectroscopy (ECS) in devices, such as handheld point-of-care devices. The devices, as well as systems and methods, utilize using Electrochemical Impedance Spectroscopy (EIS) or Electrochemical Capacitance Spectroscopy (EIS) in combination with an antibody or other target-capturing molecule on a working electrode. Imaginary impedance or phase shift, as well as background subtraction, also may be utilized.

First claim

What is claimed is:

1. An apparatus for detecting one or more analytes in a bodily fluid sample utilizing Electrochemical Impedance Spectroscopy (EIS) or Electrochemical Capacitance Spectroscopy (ECS), comprising:
an electrochemical sensor operably configured to provide an electrochemical impedance or electrochemical capacitance measurement of an analyte in said fluid, said sensor including a target-capturing molecule immobilized to a working electrode in a three electrode configuration, wherein the target-capturing molecule is an antibody, and wherein the antibody is physically adsorbed to the working electrode and covalently crosslinked by exposure to glutaraldehyde, thereby the antibody is directly immobilized onto the working electrode surface in dry form.

2. The apparatus of claim 1 , wherein said working electrode comprises one or more of a carbon conductive ink, a silver/silver chloride ink, and a mesoporous carbon ink.

3. The apparatus of claim 1 , wherein the sensor is operably configured to utilize electrochemical impedance or electrochemical capacitance to generate a calibration curve across a range of analyte concentration.

4. The apparatus of claim 3 , wherein the calibration curve is generated from a dataset of any of real impedance, imaginary impedance, complex impedance, or phase angle.

5. The apparatus of claim 1 , wherein the antibody is a lactoferrin antibody or an IgE antibody.

6. The apparatus of claim 1 , wherein the working electrode is a screen printed carbon electrode.

7. The apparatus of claim 1 , wherein the apparatus is a handheld device.

8. The apparatus of claim 1 , further comprising a tear sampling component.

9. A method for detecting one or more analytes in a bodily fluid sample utilizing Electrochemical Impedance Spectroscopy (EIS) or Electrochemical Capacitance Spectroscopy (ECS), comprising:
contacting said bodily fluid sample with the sensor of the apparatus of claim 1 ; and
measuring an electrochemical impedance or capacitance of a complex of said target-capturing molecule of the apparatus and said one or more analytes from said fluid on the working electrode of the apparatus.

10. The method of claim 9 , wherein said one or more analytes in said fluid are selected from the group consisting of IgE, Lactoferrin, MMP9, adenovirus, and glucose.

11. A system for detecting one or more analytes in a bodily fluid sample utilizing Electrochemical Impedance Spectroscopy (EIS) or Electrochemical Capacitance Spectroscopy (ECS), comprising:
the apparatus of claim 1 ; and
a reader operably configured to provide an electrochemical impedance or electrochemical capacitance measurement of a complex of said target-capturing molecule of the apparatus and said one or more analytes from said fluid on the working electrode of the apparatus.

Assignees

• Univ Arizona State
• Mayo Found Medical Education & Res
• Axim Biotechnologies Inc

Inventors

• Labelle Jeffrey
• Repp Garrett
• Lin Chi
• Spano Mark
• Christen Jennifer Blain
• Jiang Hongwu
• Smith Marcus
• Penman Andrew
• Youngbar Pierce
• Honikel Mackenzie
• Cook Curtiss

Classifications

• G01N33/5438 Primary

  Electrodes · CPC title

• G01N27/026

  Dielectric impedance spectroscopy (electrochemical impedance spectroscopy for measuring corrosion G01N17/02) · CPC title

• G01N27/3276

  being a hybridisation with immobilised receptors (using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68) · CPC title

• G01N33/48707

  by electrical means (G01N33/49, G01N33/493 take precedence) · CPC title

• G01N33/5308

  for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites · CPC title