Carbon-based particles for vapour bubble generation

Patent metadata

Field	Value
Publication number	US-11712484-B2
Application number	US-201615744181-A
Country	US
Kind code	B2
Filing date	Jun 29, 2016
Priority date	Jul 14, 2015
Publication date	Aug 1, 2023
Grant date	Aug 1, 2023

Abstract

A method for generating vapour bubbles in an object comprises introducing a composition into the object, the composition comprising carbon-based nano- or microparticles that can couple with a photon wave of electromagnetic radiation. The method also comprises irradiating said object using electromagnetic radiation. The irradiation thereby is adapted for using a set of carbon-based nano- or microparticles for subsequently forming first vapour bubbles and at least second vapour bubbles using the same carbon-based nano- or microparticles.

First claim

The invention claimed is:

1. A method for use in delivering compounds into biological cells and/or for killing of cells using irradiation of carbon-based nano- or microparticles and/or for locally altering or disrupting a microbiological film by generating vapour bubbles in an object, the method comprising:
introducing a composition into the object, the composition comprising carbon-based nano- or microparticles that can couple with a photon wave of electromagnetic radiation, the carbon-based nano- or microparticles comprising any or a combination of graphene- oxide, graphene, carbon nanotubes or carbon dots,
irradiating said object using electromagnetic radiation, wherein said irradiating comprises irradiation with a plurality of pulses and is adapted for using a set of carbon-based nano- or microparticles for forming first vapour bubbles and subsequently forming at least second vapour bubbles, wherein one of the first vapour bubbles and one of the at least second vapour bubbles is formed using the same carbon-based nano- or microparticle within the set of carbon-based nano- or microparticles,
said irradiating comprising irradiating the carbon-based nano- or microparticles during an irradiation sequence and an irradiation time and at an irradiation intensity such that the heating of the carbon-based nano- or microparticles induces subsequent formation of vapour bubbles,
wherein each of the plurality of pulses has a laser fluence greater than or equal to a threshold intensity, said threshold intensity defined as the laser fluence at which there is a 90% certainty that a bubble will be formed from said carbon-based nano- or microparticles.

2. A method for generating vapour bubbles according to claim 1 , wherein said method is an in vitro or ex vivo method.

3. A method for generating vapour bubbles according to claim 1 , wherein said carbon-based nano- or microparticles are graphene oxide particles.

4. A method for generating vapour bubbles according to claim 1 , wherein said irradiating comprises providing the plurality of irradiation pulses, wherein subsequent irradiation pulses on the carbon-based nano- or microparticles induce subsequent formation of vapour bubbles and/or wherein said irradiating comprises irradiating the carbon-based nano- or microparticles during an irradiation sequence and/or an irradiation time and/or at an irradiation intensity such that the heating of the carbon- based nano- or microparticles induces subsequent formation of vapour bubbles.

5. A method for generating vapour bubbles according to claim 1 , wherein said carbon-based nanoparticles are functionalised with drug molecules or targeting ligands, contrast agents, and/or stabilising ligands.

6. A method for generating vapour bubbles according to claim 1 , wherein
said introducing comprises allowing said carbon-based nanoparticles to diffuse into a microbiological film, and
said forming generating a mechanical force for locally altering or disrupting said microbiological film.

7. A method for generating vapour bubbles according to claim 1 , wherein the method furthermore comprises detecting acoustic signals of the vapour bubbles for imaging the object.

8. A method for generating vapour bubbles according to claim 1 , wherein each of the plurality of pulses has a duration of less than 10 ns.

9. A method for generating vapour bubbles according to claim 1 , wherein each of the nano- or microparticles have a diameter between 1 nm and 1 μm.

10. A method for generating vapour bubbles according to claim 1 , wherein the threshold intensity is determined by applying a plurality of pulses with a fluence that increases from one pulse to the next.

11. A method for generating vapour bubbles according to claim 1 , wherein the vapour bubbles have a diameter in the range of 10 nm to 100 μm.

12. A method for generating vapour bubbles according to claim 9 , wherein each of the nano- or microparticles have a diameter between 20 nm and 200 nm.

13. A method for generating vapour bubbles according to claim 1 , wherein a wavelength of the plurality of pulses is within an IR region.

14. A method for generating vapour bubbles in an object, the method comprising:
determining a threshold intensity for forming vapour bubbles from carbon-based nano- or microparticles that can couple with a photon wave of electromagnetic radiation, by applying laser pulses at increasing fluence levels from 20 to 470 mJ/cm 2 to said carbon-based nano- or microparticles until reaching the threshold intensity at which there is a 90% certainty that a bubble will be formed,
introducing a composition into the object, the composition comprising said carbon-based nano- or microparticles, the carbon-based nano- or microparticles comprising graphene-oxide, graphene, carbon nanotubes or carbon dots,
irradiating said object using electromagnetic radiation, wherein said irradiating comprises irradiation with a plurality of pulses and is adapted for using a set of carbon-based nano- or microparticles for forming first vapour bubbles and subsequently forming at least second vapour bubbles, wherein one of the first vapour bubbles and one of the at least second vapour bubbles is formed using the same carbon-based nano- or microparticle within the set of carbon-based nano- or microparticles,
wherein said object comprises a biological system, and the method is for use in delivering compounds into biological cells and/or for killing of cells using said irradiation of said carbon-based nano- or microparticles and/or for locally altering or disrupting a microbiological film, and
wherein each of the plurality of pulses has a laser fluence greater than or equal to the threshold intensity.

15. A method for generating vapour bubbles in an object, the method comprising:
introducing a composition into the object, the composition comprising carbon-based nano- or microparticles that can couple with a photon wave of electromagnetic radiation, the carbon-based nano- or microparticles comprising graphene-oxide,
irradiating said object using electromagnetic radiation, wherein said irradiating comprises irradiation with a plurality of pulses and is adapted for using a set of carbon-based nano- or microparticles for forming first vapour bubbles and subsequently forming at least second vapour bubbles, wherein one of the first vapour bubbles and one of the at least second vapour bubbles is formed using the same carbon-based nano- or microparticle within the set of carbon-based nano- or microparticles,
wherein said object comprises a biological system, and the method is for use in delivering compounds into biological cells and/or for killing of cells using said irradiation of said carbon-based nano- or microparticles and/or for locally altering or disrupting a microbiological film, and
wherein each of the plurality of pulses has a fluence of 500 mJ/cm 2 , a pulse duration of 9 ns or less, and a pulse interval of 0.2 seconds.

Assignees

• Univ Gent
• Centre Nat Rech Scient
• Univ Des Sciences Et Technologies De Lille 1

Inventors

• Braeckmans Kevin
• De Smedt Stefaan
• Demeester Joseph
• Samal Sangram Keshari
• Xiong Ranhua
• Szunerits Sabine
• Boukherroub Rabah

Classifications

• A61K49/225 Primary

  Microparticles, microcapsules (gas-filled to be classified in A61K49/223) · CPC title

• A61K33/44

  Elemental carbon, e.g. charcoal, carbon black · CPC title

• A61K41/00

  Medicinal preparations obtained by treating materials with wave energy or particle radiation {; Therapies using these preparations} · CPC title

• A61K41/0028

  Disruption, e.g. by heat or ultrasounds, sonophysical or sonochemical activation, e.g. thermosensitive or heat-sensitive liposomes, disruption of calculi with a medicinal preparation and ultrasounds · CPC title

• A61K47/02

  Inorganic compounds · CPC title