Light-sheet fluorescence imaging with elliptical light shaping diffuser

Patent metadata

Field	Value
Publication number	US-11709351-B2
Application number	US-201816763158-A
Country	US
Kind code	B2
Filing date	Nov 6, 2018
Priority date	Nov 13, 2017
Publication date	Jul 25, 2023
Grant date	Jul 25, 2023

Abstract

Systems and methods for passive multi-directional illumination in light-sheet fluorescence imaging and microscopy are disclosed herein. An elliptical light shaping diffuser is placed in the illumination path between the source of a light-sheet and the illuminated sample. The light-sheet is diffused anisotropically along two directions perpendicular to its propagation direction, eliminating stripe artifacts in obtained images. The method includes converting a light-sheet into an elliptically diffuse light-sheet by passing it through an elliptical light shaping diffuser, illuminating a sample with the elliptically diffuse light-sheet. The system includes a light-sheet source, an elliptical light shaping diffuser adapted to convert the light-sheet into an elliptically diffuse light-sheet to illuminate the sample, typical microscopy optics and lenses, and image capturing elements.

First claim

The invention claimed is:

1. A method of light-sheet fluorescence imaging comprising:
providing a sample;
providing a parallel-light light sheet;
creating an elliptically diffuse light-sheet by passing the parallel-light light sheet through an elliptical light-shaping diffuser to produce a passive multi-directional illumination which circumvents scattering and absorbing objects and results in decreased formation of shadows and stripe artifacts in the at least one image of the sample compared to an image of the sample illuminated with single-directional illumination;
wherein the elliptically diffuse light sheet is configured to transmit a first plurality of light rays in a direction parallel to the parallel-light light sheet and a second plurality of light rays in a direction perpendicular to the parallel-light light sheet;
illuminating the sample with the elliptically diffuse light-sheet;
capturing at least one image of the sample.

2. The method of claim 1 , wherein the at least one image does not exhibit shadows or stripe artifacts.

3. The method of claim 1 , wherein the elliptical light shaping diffuser is one of an elliptical holographic diffuser and a line diffuser.

4. The method of claim 1 , wherein the elliptical light shaping diffuser is adapted to transform an input light-sheet into an output light-sheet that spreads anisotropically along orthogonal diffusing angles.

5. The method of claim 4 , wherein the sample has one or more surface features to be imaged, and wherein the one or more surface features to be imaged have a size larger than a width of the elliptically diffuse light-sheet upon illumination of the sample, wherein the width is measured in a direction perpendicular to a plane defined by the parallel light sheet.

6. The method of claim 4 , wherein the elliptically diffuse light-sheet spreads at a larger diffusing angle in a plane parallel to the parallel light-sheet than in a plane perpendicular to the parallel light-sheet.

7. A system for light-sheet fluorescence imaging of a fluorescent sample comprising:
a light source adapted to create a parallel-light light-sheet;
an elliptical light shaping diffuser adapted to convert the light-sheet into an elliptically diffuse light-sheet by passing the parallel-light light-sheet through the elliptical light shaping diffuser, the elliptically diffuse light-sheet comprising a first plurality of light rays oriented parallel to the parallel-light light-sheet and a second plurality of light rays oriented perpendicular to the parallel-light sheet and illuminate the specimen;
at least one microscopy optic;
an image capturing element;
wherein the elliptical light shaping diffuser is configured to produce a passive multi-directional illumination which circumvents scattering and absorbing objects and wherein the multi-directional illumination results in decreased formation of shadows and stripe artifacts in at least one image of the sample compared to an image of the sample illuminated with single-directional illumination.

8. The system of claim 7 , wherein the elliptical light shaping diffuser is at least one of an elliptical holographic diffuser and a line diffuser.

9. The system of claim 7 , wherein the at least one microscopy optic is part of a microscope.

10. The system of claim 7 , wherein images captured by the image capturing element do not exhibit shadows or stripe artifacts.

11. The system of claim 7 , wherein the light source provides a substantially monochromatic beam.

12. The system of claim 11 , wherein the substantially monochromatic beam is passed through a laser line generator and is shaped into a line.

13. The system of claim 11 , wherein the wavelength of the substantially monochromatic beam is within absorption range of the fluorescent sample.

14. The system of claim 7 , wherein the elliptical light shaping diffuser is located between the light source and the fluorescent sample.

15. The system of claim 14 , wherein the elliptical light shaping diffuser is located closer to the fluorescent sample than to the light source.

16. The system of claim 15 , wherein the parallel light-sheet is passed through the elliptical light shaping diffuser at a location that can vary based on properties of the at least one microscopy optic.

17. A system for light-sheet fluorescence imaging of a fluorescent sample comprising:
a light source adapted to create a parallel-light light-sheet;
an elliptical light shaping diffuser adapted to convert the light-sheet into an elliptically diffuse light-sheet, comprising a first plurality of light rays oriented parallel to the parallel-light light-sheet and a second plurality of light rays oriented perpendicular to the parallel-light sheet and illuminate the specimen;
at least one microscopy optic;
an image capturing element;
wherein a diffusion angle of the second plurality of light rays oriented perpendicular to the parallel-light sheet is less than a divergence of a thickness of the parallel-light light sheet, configured such that the elliptical light shaping diffuser does not increase the divergence of the parallel-light sheet.

Assignees

• Univ Pennsylvania

Inventors

• Salili Seyyed
• Durian Douglas
• Harrington Matthew

Classifications

• G02B21/06 Primary

  Means for illuminating specimens · CPC title

• G02B21/0076

  arrangements using fluorescence or luminescence · CPC title

• G02B21/36

  arranged for photographic purposes or projection purposes (G02B21/18 takes precedence){or digital imaging or video purposes including associated control and data processing arrangements (image data processing per se G06T)} · CPC title

• G02B21/367 Primary

  providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison · CPC title

• G02B21/0032

  Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers (G02B21/0036 - G02B21/008; means for illumination of specimens in general G02B21/06) · CPC title