System and method for sperm sorting

Patent metadata

Field	Value
Publication number	US-11709122-B2
Application number	US-202117322464-A
Country	US
Kind code	B2
Filing date	May 17, 2021
Priority date	Nov 20, 2013
Publication date	Jul 25, 2023
Grant date	Jul 25, 2023

Abstract

A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

First claim

The invention claimed is:

1. A system for sorting sperm, comprising:
a housing including a lower component and an upper component coupled together;
a fluidic system supported by the housing;
an inlet extending through the lower component and providing access to the fluidic system to deliver sperm to the fluidic system;
a collection chamber configured to pass motile sperm for harvesting and restrict non-motile sperm, the collection chamber including a first chamber extending through the lower component and a second chamber extending through the upper component and positioned above the first chamber;
at least one channel extending from the inlet to the collection chamber to allow sperm delivered to the fluidic system through the inlet to progress along a flow path toward the collection chamber;
a filter including a plurality of micropores and arranged in the collection chamber to cause sperm traveling along the flow path to move through the filter and against gravity to reach the second chamber, wherein the filter is located between the upper component and the lower component; and
an additional collection chamber extending into the upper component and connected to the second chamber, the additional collection chamber sized and configured to facilitate harvesting functional sperm out of the additional collection chamber, wherein the functional sperm are suitable for fertilization and are at least some of the motile sperm.

2. The system of claim 1 , wherein the plurality of micropores are sized to permit a head of the sperm to pass therethrough.

3. The system of claim 1 , wherein the plurality of micropores are at least 1 um and less than 10 um in diameter.

4. The system of claim 1 , wherein the fluidic system is configured such that some of the motile sperm pass through the filter into the second chamber and traverse a path from the second chamber into the additional collection chamber in a flow-free manner, and wherein the second chamber is connected to the additional collection chamber by a fluid connection channel and no other channels extend from the second chamber.

5. The system of claim 4 , wherein the additional collection chamber does not comprise a filter.

6. The system of claim 1 , wherein a top portion of the second chamber of the collection chamber is an open outlet that provides access to the fluidic system.

7. The system of claim 6 , further comprising sperm arranged at the inlet.

8. The system of claim 6 , further comprising sperm within the at least one channel but not above the filter.

9. The system of claim 1 , wherein the additional collection chamber does not comprise a filter.

10. The system of claim 1 , further comprising a slide supporting the housing, wherein the slide is located under the lower component and forms a lower surface of the at least one channel.

11. The system of claim 1 , wherein the filter is a polycarbonate filter.

12. The system of claim 1 , wherein the second chamber is connected to the additional collection chamber by a fluid connection channel and no other channels extend from the second chamber.

13. A method for sorting sperm, comprising:
delivering a sample of sperm into an inlet connected to a fluidic system comprising a housing including a lower component and an upper component coupled together, wherein the inlet extends through the lower component and provides access to the fluidic system to deliver sperm to the fluidic system;
allowing sperm in the sample of sperm to traverse a flow path through the fluidic system from the inlet to a collection chamber, wherein the collection chamber includes a first chamber formed by the lower component, and a second chamber positioned above the first chamber and formed by the upper component, wherein the first chamber and the second chamber are separated by a filter positioned in the collection chamber, wherein the filter has a plurality of micropores sized to permit a head of the sperm to pass therethrough, and wherein the second chamber is connected to an additional collection chamber by a fluid connection channel and no other channels extend from the second chamber;
allowing at least some motile sperm from the sample of sperm that have entered the collection chamber to selectively pass through the filter against gravity to traverse a path into the second chamber and then to traverse a path into an additional collection chamber; and
harvesting functional sperm from the additional collection chamber, wherein the functional sperm are suitable for fertilization and are at least some of the motile sperm that have passed through the filter and into the additional collection chamber, wherein the fluidic system is configured such that some of the motile sperm pass through the filter into the second chamber and move from the second chamber into the additional collection chamber in a flow-free manner.

14. The method of claim 13 , wherein the first chamber extends through the lower component and the second chamber extends through the upper component.

15. The method of claim 14 , wherein the filter is located between the upper component and the lower component.

16. The method of claim 15 , wherein a top of the second chamber is an open outlet that provides access to the fluidic system as the motile sperm traverse the flow path through the fluidic system from the inlet to the collection chamber.

17. The method of claim 15 , wherein the collection chamber is connected to the additional collection chamber by a fluid connection channel, and no other channels extend from the additional collection chamber.

18. The method of claim 15 , wherein the additional collection chamber does not comprise a filter when the at least some of the motile sperm are harvested.

19. The method of claim 13 , wherein a top of the second chamber is an open outlet that provides access to the fluidic system as the motile sperm traverse the flow path through the fluidic system from the inlet to the collection chamber.

20. The method of claim 13 , wherein a higher percentage of motile sperm are present at the additional collection chamber than in the sample when the at least some of the motile sperm are harvested.

21. The method of claim 13 , wherein the additional collection chamber does not comprise a filter when the at least some of the motile sperm are harvested.

22. The method of claim 13 , wherein the plurality of micropores are at least 1 um and less than 10 um in diameter.

23. The method of claim 22 , wherein the filter is located between the upper component and the lower component.

Assignees

• Brigham & Womens Hospital Inc

Inventors

• Demirci Utkan
• Asghar Waseem

Classifications

• B01L3/502707

  characterised by the manufacture of the container or its components · CPC title

• B01L3/502753

  characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title

• B01L2300/0887

  Laminated structure · CPC title

• B01L2300/0681

  Filter · CPC title