Method for preparing natural L-cysteine crystals by continuous chromatography

Patent metadata

Field	Value
Publication number	US-11708591-B2
Application number	US-201916957921-A
Country	US
Kind code	B2
Filing date	Jan 30, 2019
Priority date	Jan 31, 2018
Publication date	Jul 25, 2023
Grant date	Jul 25, 2023

Abstract

The present disclosure relates to a method for preparing L-cysteine crystals, and L-cysteine crystals prepared by the method. Through the method for preparing L-cysteine crystals of the present disclosure, L-cysteine crystals can be obtained from a natural L-cysteine fermentation broth with a high recovery rate and/or purity without a chemical reaction or the use of an artificial synthetic compound.

First claim

The invention claimed is:

1. A method for preparing L-cysteine crystals, comprising:
(a) obtaining a separated liquid after introducing a fermentation broth in a pH range of 3.0 to 9.0 containing L-cysteine into a continuous chromatography apparatus having a strongly acidic cation-exchange resin as a stationary phase, wherein the continuous chromatography excludes adsorption or elution of L-cysteine;
(b) concentrating the separated liquid; and
(c) recovering L-cysteine crystals from the concentrate.

2. The method of claim 1 , further comprising adjusting the fermentation broth containing L-cysteine to a pH of 3.5 to 7.5 prior to step (a).

3. The method of claim 1 , further comprising concentrating the fermentation broth in a pH range of 3.0 to 9.0 containing L-cysteine prior to step (a).

4. The method of claim 1 , wherein the strongly acidic cation-exchange resin in step (a) has a sulfonic acid functional group.

5. The method of claim 1 , wherein the strongly acidic cation-exchange resin in step (a) is a styrene-divinylbenzene copolymer having a sulfonic acid functional group.

6. The method of claim 1 , wherein the continuous chromatography apparatus in step (a) is a simulated moving bed (SMB) chromatography apparatus.

7. The method of claim 1 , wherein the separated liquid in step (a) has a solid content of L-cysteine excluding moisture of 85% (w/w) or more.

8. The method of claim 1 , wherein the yield of continuous chromatography in step (a), as a ratio of L-cysteine in the separated liquid obtained relative to the fermentation broth introduced, is 50% (w/w).

9. The method of claim 1 , wherein step (b) is carried out such that the concentration of L-cysteine in the separated liquid is from 200 g/L to less than 800 g/L.

10. The method of claim 1 , wherein step (b) is carried out such that the concentration of L-cysteine in the separated liquid is from 300 g/L to less than 700 g/L.

11. The method of claim 1 , further comprising cooling the concentrate prior to step (c).

12. The method of claim 11 , wherein the concentrate is cooled to a temperature of 0° C. to 30° C.

13. The method of claim 1 , comprising adding a filtrate obtained by recovering the crystals in step (c) to the fermentation broth of step (a) or the separated liquid of step (b).

14. The method of claim 1 , wherein the purity of the prepared L-cysteine crystals is 98% (w/w) or more.

15. L-Cysteine crystals prepared according to the preparation method of claim 1 .

Assignees

• Cj Cheiljedang Corp

Inventors

• Kim Jun-Woo
• Lee Jung Min
• Jo Se-Hee
• Kim Il Chul
• Lee In Sung
• Jung Jun Young

Classifications

• B01D15/1821

  Simulated moving beds · CPC title

• C12P13/12 Primary

  Methionine; Cysteine; Cystine · CPC title

• B01D15/1892

  the sorbent material moving as a whole, e.g. continuous annular chromatography, true moving beds or centrifugal chromatography · CPC title

• B01D15/362

  Cation-exchange · CPC title

• C08L25/18

  Homopolymers or copolymers of aromatic monomers containing elements other than carbon and hydrogen · CPC title