Production bacterial cells and use thereof in production methods

Patent metadata

Field	Value
Publication number	US-11697802-B2
Application number	US-202217742671-A
Country	US
Kind code	B2
Filing date	May 12, 2022
Priority date	May 12, 2021
Publication date	Jul 11, 2023
Grant date	Jul 11, 2023

Abstract

The present invention concerns a production bacterial cell for producing phage particles or phage-derived delivery vehicles, said production bacterial cell stably comprising at least one phage structural gene(s) and at least one phage DNA packaging gene(s), said phage structural gene(s) and phage DNA packaging gene(s) being derived from a first type of bacteriophage,wherein the expression of at least one of said phage structural gene(s) and/or at least one of said phage DNA packaging gene(s) in said production bacterial cell is controlled by at least one induction mechanism, andwherein said production bacterial cell is from a bacterial species or strain different from the bacterial species or strain from which said first type of bacteriophage comes and/or that said first type of bacteriophage targets.

First claim

The invention claimed is:

1. Production bacterial cell for producing phage particles or phage-derived delivery vehicles, said production bacterial cell stably comprising phage structural genes and phage DNA packaging genes of a first type of bacteriophage,
wherein the expression of said phage structural genes and phage DNA packaging genes in said production bacterial cell is controlled by an induction mechanism comprising phage excision/insertion genes, phage DNA replication genes, and phage regulation genes of a second, different type of bacteriophage, wherein said phage excision/insertion genes, phage DNA replication genes and phage regulation genes are neither phage DNA packaging genes nor phage structural genes,
wherein said production bacterial cell does not comprise phage excision/insertion genes and/or phage replication genes of the first type of bacteriophage,
wherein said production bacterial cell is from a bacterial species or strain different from the bacterial species or strain from which said first type of bacteriophage comes from and/or that said first type of bacteriophage targets and wherein said production bacterial cell is from the same bacterial species or strain as the bacterial species or strain from which said second type of bacteriophage comes from and/or that said second type of bacteriophage targets,
wherein said production bacterial cell is a P. freudenreichii bacterial cell,
wherein the first type of bacteriophage is a C. acnes phage and
wherein the second type of bacteriophage is a P. freudenreichii phage.

2. The production bacterial cell according to claim 1 , wherein said bacterial cell further comprises a payload to be packaged into said phage particles or phage-derived delivery vehicles.

3. The production bacterial cell according to claim 2 , wherein said payload is a nucleic acid payload comprising a packaging site derived from said first type of bacteriophage.

4. The production bacterial cell according to claim 2 , wherein said payload is to be delivered into targeted bacterial cells.

5. The production bacterial cell according to claim 4 , wherein said payload comprises a sequence of interest.

6. The production bacterial cell according to claim 5 , wherein said sequence of interest only generates an effect in said targeted bacterial cells.

7. The production bacterial cell according to claim 6 , wherein said targeted bacterial cells are from a species or strain different from the production bacterial cell.

8. The production bacterial cell of claim 1 , wherein said phage structural genes and phage DNA packaging genes of said first type of bacteriophage are comprised in at least one plasmid, chromosome and/or helper phage.

9. The production bacterial cell according to claim 1 , comprising the entire structural operon of the first type of bacteriophage.

Assignees

• Eligo Bioscience

Inventors

• Fernandez Rodriguez Jesus
• Decrulle Antoine
• Leveau Aymeric
• Canadas Blasco Ines
• Mathieu Aurélie
• Carlier Thibault

Classifications

• C12N15/70 Primary

  Vectors or expression systems specially adapted for E. coli · CPC title

• C12N15/74

  Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora · CPC title

• C12N2800/101

  for bacteria · CPC title

• C12N2800/40

  Systems of functionally co-operating vectors · CPC title

• C12N7/00 Primary

  Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00) · CPC title