Silk fibroin biocompatible polyurethane membranes

Patent metadata

Field	Value
Publication number	US-11684695-B2
Application number	US-201716089243-A
Country	US
Kind code	B2
Filing date	Apr 13, 2017
Priority date	Apr 14, 2016
Publication date	Jun 27, 2023
Grant date	Jun 27, 2023

Abstract

The present invention relates to the preparation of a membrane for use in the repair of the middle ear including perforations and damage to the tympanic membrane. More particularly, the invention provides for compositions and methods for preparing silk fibroin biocompatible polyurethane membranes using a solvent, which have improved biodegradation, mechanical and vibroacoustic properties.

First claim

The invention claimed is:

1. A silk fibroin biocompatible polyurethane membrane matrix for use as a tympanic membrane, or as a scaffold for the regeneration of a tympanic membrane, wherein the membrane matrix:
(a) includes a blend of silk fibroin and at least 90% w/w biocompatible polyurethane;
(b) transmits sound waves between 20 Hz and 20 KHz to the middle ear in vivo; and
(c) has a tensile strength between 5 MPa to 100 MPa.

2. The silk fibroin biocompatible polyurethane membrane matrix according to claim further comprising a plurality of membrane matrices.

3. A method of fabricating a silk fibroin biocompatible polyurethane membrane matrix according to claim 1 comprising the steps of:
(a) preparing silk protein or a silk protein complex solution after removal of sericin from a cocoon or fibre;
(b) dissolving biocompatible polyurethane and silk protein using a solvent; and
(c) drying the prepared solution to fabricate a silk protein biocompatible polyurethane membrane matrix having at least 90% w/w biocompatible polyurethane.

4. A silk fibroin biocompatible polyurethane membrane matrix prepared according to the method of claim 3 .

5. A silk fibroin biocompatible polyurethane membrane matrix according to claim 1 , comprising at least one active agent.

6. A silk fibroin biocompatible polyurethane membrane matrix according to claim 5 , wherein the active agent is selected from the group consisting of: cells, proteins, peptides, nucleic acid analogues, nucleotides or oligonucleotides, peptide nucleic acids, aptamers, antibodies or fragments or portions thereof, hormones, hormone antagonists, growth factors or recombinant, growth factors and fragments and variants thereof, cytokines, enzymes, antibiotics or antimicrobial compounds, viruses, antivirals, toxins, prodrugs, chemotherapeutic agents, small molecules, drugs, and combinations thereof.

7. The silk fibroin biocompatible polyurethane membrane matrix according to claim 5 , wherein the membrane supports growth of keratinocytes, fibroblasts, mucosal epithelium, endothelial cells, chondrocytes, induced pluripotent stem cells, adult stem cells and embryonic stem cells, and combinations thereof.

8. The silk fibroin biocompatible polyurethane membrane matrix according to claim 5 , wherein the membrane matrix is resistant to degradation and/or provides long term structural support to resist retraction, atelectasis and cholesteatoma.

9. A device comprising the silk fibroin biocompatible polyurethane membrane matrix according to claim 1 .

10. The device according to claim 9 , further comprising a plurality of silk fibroin biocompatible polyurethane membrane matrices.

11. A silk fibroin biocompatible polyurethane membrane matrix according to claim 4 , comprising at least one active agent.

12. The silk fibroin biocompatible polyurethane membrane matrix according to claim 11 , wherein the membrane supports growth of keratinocytes, fibroblasts, mucosal epithelium, endothelial cells, chondrocytes, induced pluripotent stem cells, adult stem cells and embryonic stem cells, and combinations thereof.

13. The silk fibroin biocompatible polyurethane membrane matrix according to claim 11 , wherein the membrane matrix is resistant to degradation and/or provides long term structural support to resist retraction, atelectasis and cholesteatoma.

14. The device according to claim 9 and further comprising at least one active agent.

15. The silk fibroin biocompatible polyurethane membrane matrix of claim 1 wherein the biocompatible polyurethane comprises a dihydroxypropyl poly(dimethylsiloxane) moiety.

16. A silk fibroin biocompatible polyurethane membrane matrix comprising a blend of silk fibroin and
at least 90% w/w of a biocompatible polyurethane comprising a dihydroxypropyl poly(dimethylsiloxane) moiety,
wherein the membrane matrix transmits sound waves between 20 Hz and 20 KHz to the middle ear in vivo and has a tensile strength between 5 MPa and 100 MPa.

Assignees

• Ear Science Inst Australia
• Univ Deakin

Inventors

• Atlas Marcus
• Allardyce Benjamin
• Dilley Rodney
• Rajkhowa Rangam

Classifications

• A61L27/3604 Primary

  characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel · CPC title

• A61F2/18

  Internal ear or nose parts, e.g. ear-drums {(implants for middle ear ventilation or drainage A61F11/202)} · CPC title

• A61L27/54

  Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)} · CPC title

• A61L27/225

  Fibrin; Fibrinogen · CPC title

• A61L2300/40

  characterised by a specific therapeutic activity or mode of action · CPC title