Electric field stirring apparatus, electric field stirring method, and pathological sample manufacturing apparatus

Patent metadata

Field	Value
Publication number	US-11680943-B2
Application number	US-201916590499-A
Country	US
Kind code	B2
Filing date	Oct 2, 2019
Priority date	Oct 3, 2018
Publication date	Jun 20, 2023
Grant date	Jun 20, 2023

Abstract

An electric field stirring apparatus, in which a droplet as a liquid disposed between a first electrode and a second electrode disposed to face each other is vibrated and stirred by an electric field generated between the first electrode and the second electrode, the second electrode having a groove formed along a first direction on an electrode surface facing the first electrode, includes a movement mechanism that reciprocally moves the first electrode relative to the second electrode in a second direction intersecting the first direction in a state in which the first electrode and the second electrode face each other during a period in which the electric field is generated.

First claim

What is claimed is:

1. A pathological sample manufacturing apparatus comrpsing:
an electric field stirring apparatus in which a liquid disposed between a first electrode and a second electrode disposed to face each other is vibrated and stirred by an electric field generated between the first electrode and the second electrode, the second electrode having a groove formed along a first direction on an electrode surface facing the first electrode, the electric field stirring apparatus including a movement mechanism that reciprocally moves at least one of the first electrode and the second electrode relative to the other electrode in a second direction intersecting the first direction in a state in which the first electrode and the second electrode face each other during a period in which the electric field is generated;
a stage section that includes a stage which functions as the first electrode of the electric field stirring apparatus and on which a substrate with a tissue sample fixed to a predetermined region is mounted;
a reagent supply section that supplies a reagent to the substrate mounted on the stage;
a cleaning section that supplies a cleaning liquid to the substrate mounted on the stage;
a stage transport mechanism that functions as the movement mechanism of the electric field stirring apparatus when the cleaning section, the reagent supply section, and the electric field stirring apparatus are disposed in the second direction, and moves the stage in the second direction; and
a control unit, wherein based on a pathological sample manufacturing protocol, the control unit controls driving of the stage transport mechanism, thus moves the stage to position facing the second electrode of the electric field stirring apparatus, and subjects the reagent or the cleaning liquid supplied to the substrate to electric field stirring.

2. The pathological sample manufacturing apparatus according to claim 1 , wherein
the liquid is disposed in a predetermined region on one surface of a substrate,
the first electrode has a mounting portion on which the substrate is mounted, and
when a position where a center of the predetermined region of the substrate mounted on the mounting portion of the first electrode faces a center of the groove of the second electrode is set as an origin in the second direction, the movement mechanism reciprocally moves the one electrode in the second direction relative to the other electrode to a position where an inner edge of the predetermined region is deviated relative to the origin.

3. The pathological sample manufacturing apparatus according to claim 2 , wherein
a plurality of the grooves are formed with a predetermined arrangement pitch in the second direction on the electrode surface of the second electrode, and
the predetermined region in which the liquid is disposed is provided in a plurality with the predetermined arrangement pitch in the second direction on the one surface of the substrate.

4. The pathological sample manufacturing apparatus according to claim 1 , further comprising:
an electric field generation section that periodically applies a voltage between the first electrode and the second electrode so as to generate an electric field;
a detection section that detects the voltage applied between the first electrode and the second electrode; and
a control section, wherein
the control section controls the electric field generation section such that a value of the voltage detected by the detection section is included in a predetermined range.

5. The pathological sample manufacturing apparatus according to claim 4 , wherein
the predetermined range of the value of the voltage is ±5% of a predetermined voltage value.

6. The pathological sample manufacturing apparatus according to claim 1 , wherein
a plurality of the stage sections are provided,
the stage transport mechanism is provided to correspond to each of the plurality of stage sections, and
the second electrode of the electric field stirring apparatus is separately provided for each of the plurality of stage sections.

7. The pathological sample manufacturing apparatus according to claim 1 , further comprising:
a probe that is brought into contact with the stage transported to the electric field stirring apparatus by the stage transport mechanism, and applies a potential for generating an electric field, wherein
the electric field stirring apparatus includes a probe movement mechanism that reciprocally moves the probe in the second direction in a state in which the probe is brought into contact with the stage.

8. An electric field stirring method comprising:
an electric field stirring step of vibrating and stirring a liquid disposed between a first electrode and a second electrode disposed to face each other with an electric field generated between the first electrode and the second electrode, wherein
the second electrode has a groove formed along a first direction on an electrode surface facing the first electrode, and
in the electric field stirring step, at least one of the first electrode and the second electrode is reciprocally moved relative to the other electrode in a second direction intersecting the first direction in a state in which the first electrode and the second electrode face each other during a period in which the electric field is generated.

9. The electric field stirring method according to claim 8 , wherein
the liquid is disposed in a predetermined region on one surface of a substrate, and
in the electric field stirring step, when a position where a center of the predetermined region of the substrate mounted on the first electrode faces a center of the groove of the second electrode is set as an origin in the second direction, the one electrode is reciprocally moved in the second direction relative to the other electrode to a position where an outer edge of the predetermined region is deviated relative to the origin.

10. The electric field stirring method according to claim 9 , wherein
a plurality of the grooves are formed with a predetermined arrangement pitch in the second direction on the electrode surface of the second electrode, and
the predetermined region in which the liquid is disposed is provided in a plurality with the predetermined arrangement pitch in the second direction on the one surface of the substrate.

11. The electric field stirring method according to claim 8 , wherein
in the electric field stirring step, a voltage is periodically applied between the first electrode and the second electrode so as to generate an electric field, and the voltage is applied such that a value of the voltage applied between the first electrode and the second electrode is included in a predetermined range.

Assignees

• Seiko Epson Corp

Inventors

• Aoki Taro
• Sato Tadashi
• Suzuki Yoichi
• Enomoto Junya

Classifications

• B01F33/3021

  the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets · CPC title

• G01N33/5302 Primary

  Apparatus specially adapted for immunological test procedures · CPC title

• B01F33/051

  the energy being electrical energy working on the ingredients or compositions for mixing them · CPC title

• B01F33/05

  Mixers using radiation, e.g. magnetic fields or microwaves to mix the material ({B01F23/2133, B01F23/238, B01F23/55,} B01F33/3031, B01F33/3032 take precedence) · CPC title

• B01F33/3031

  using electro-hydrodynamic [EHD] or electro-kinetic [EKI] phenomena to mix or move the fluids · CPC title