Biological analysis devices and systems

Patent metadata

Field	Value
Publication number	US-11680905-B2
Application number	US-202217572926-A
Country	US
Kind code	B2
Filing date	Jan 11, 2022
Priority date	Jun 19, 2019
Publication date	Jun 20, 2023
Grant date	Jun 20, 2023

Abstract

A biological analysis system can include an excitation module and an emission module. The excitation module can include a collimator element for receiving excitation light from the excitation light source and transmitting collimated excitation light in a first direction, and a plurality of excitation mirrors arrayed along the excitation light path, each excitation mirror disposed at an acute angle relative to the first direction and configured to reflect collimated excitation light along a second direction. The emission module can be positioned to receive excitation light transmitted along the second direction and can include a sample block comprising a plurality of sample receptacles positioned to receive a beam of collimated excitation light, and a plurality of photodetectors configured to receive emission light transmitted from a respective sample receptacle in a direction transverse to the second direction of the excitation light path.

First claim

What is claimed is:

1. A biological analysis system comprising:
an excitation module configured to direct excitation light along an excitation light path in a first direction, the excitation module comprising:
one or more excitation mirrors arrayed along the excitation light path, each configured to reflect a respective beam of excitation light along a second direction of the excitation light path; and
a plurality of excitation lenses, each excitation lens disposed in the second direction; and

an emission module positioned to receive excitation light transmitted along the second direction of the excitation light path, the emission module comprising:
a plurality of sample receptacles, each sample receptacle positioned to receive a respective beam of excitation light transmitted along the second direction of the excitation light path from an excitation lens; and
one or more photodetectors, each photodetector configured to receive emission light transmitted in a third direction from a respective sample receptacle, the third direction being transverse to the second direction of the excitation light path.

2. The biological analysis system of claim 1 , wherein the emission module comprises a plurality of photodetectors and wherein each photodetector is oriented in the third direction toward the respective sample receptacle.

3. The biological analysis system of claim 1 , wherein the third direction is substantially orthogonal to the second direction of the excitation light path.

4. The biological analysis system of claim 2 , wherein the emission module additionally comprises a plurality of emission lenses configured to focus emission light transmitted in the third direction onto the plurality of photodetectors.

5. The biological analysis system of claim 4 , wherein the emission module additionally comprises a plurality of emission filters corresponding to the plurality of emission lenses, the plurality of emission filters being positioned downstream of the corresponding plurality of emission lenses and configured to allow emission light to pass through the emission filter and to substantially block stray excitation light.

6. The biological analysis system of claim 5 , wherein the plurality of emission filters comprise dual bandpass filters.

7. The biological analysis system of claim 4 , wherein each emission lens comprises a curved lens.

8. The biological analysis system of claim 1 , wherein the emission module further comprises a plurality of emission windows, each emission window associated with a respective sample receptacle and defining an area through which emission light is transmitted to downstream components in the third direction.

9. The biological analysis system of claim 1 , wherein at least one of the one or more excitation mirrors is independently adjustable.

10. The biological analysis system of claim 1 , wherein the excitation module comprises a plurality of excitation mirrors and wherein the plurality of excitation mirrors are arrayed in a staggered, diagonal pattern.

11. A biological analysis system comprising:
an excitation module configured to direct excitation light along an excitation light path in a first direction, the excitation module comprising:
one or more excitation mirrors arrayed along the excitation light path, each configured to reflect a respective beam of excitation light along a second direction of the excitation light path; and

an emission module positioned to receive excitation light transmitted along the second direction of the excitation light path, the emission module comprising:
a plurality of sample receptacles, each sample receptacle positioned to receive a respective beam of excitation light transmitted along the second direction of the excitation light path from an excitation lens;
one or more photodetectors, each photodetector configured to receive emission light transmitted in a third direction from a respective sample receptacle, the third direction being transverse to the second direction of the excitation light path; and
a plurality of emission lenses configured to focus emission light onto the one or more photo detectors.

12. The biological analysis system of claim 11 , wherein the third direction is substantially orthogonal to the second direction of the excitation light path.

13. The biological analysis system of claim 11 , wherein the emission module additionally comprises a plurality of emission filters corresponding to the plurality of emission lenses, the plurality of emission filters being positioned downstream of the corresponding plurality of emission lenses and configured to allow emission light to pass through the emission filter and to substantially block stray excitation light.

14. The biological analysis system of claim 11 , wherein the plurality of emission filters comprise dual bandpass filters.

15. The biological analysis system of claim 11 , wherein at least one of the one or more excitation mirrors is independently adjustable.

16. The biological analysis system of claim 11 , wherein the excitation module comprises a plurality of excitation mirrors and wherein the plurality of excitation mirrors are arrayed in a staggered, diagonal pattern.

17. The biological analysis system of claim 11 , wherein each emission lens comprises a curved lens.

18. A biological analysis system comprising:
an excitation module configured to direct excitation light along an excitation light path in a first direction, the excitation module comprising:
a plurality of excitation mirrors arrayed along the excitation light path, each configured to reflect a respective beam of excitation light along a second direction of the excitation light path,
wherein the excitation mirrors are arranged in a diagonal pattern; and

an emission module positioned to receive excitation light transmitted along the second direction of the excitation light path, the emission module comprising:
a plurality of sample receptacles, each sample receptacle positioned to receive a respective beam of excitation light transmitted along the second direction of the excitation light path from an excitation lens; and
one or more photodetectors, each photodetector configured to receive emission light transmitted in a third direction from a respective sample receptacle, the third direction being transverse to the second direction of the excitation light path.

19. The biological analysis system of claim 18 , wherein the diagonal pattern of the excitation mirrors is staggered.

20. The biological analysis system of claim 18 , wherein at least one of the excitation mirrors is independently adjustable.

Assignees

• Life Tech Holdings Pte Limited
• Life Technologies Corp
• Life Tech Holdings Pte Ltd

Inventors

• Chen Ming Song
• Chong Chee Woei
• Loh Wuh Ken
• Foo Wern Yuh
• Boo Kuan Moon
• Leclerc Norbert
• Uhlendorf Kristina
• Koellner Reiner
• Fuchs Torsten
• Breitfelder Stefan

Classifications

• G01N21/6428 Primary

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title

• G01N21/6458

  Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16) · CPC title

• G01N21/6452 Primary

  Individual samples arranged in a regular 2D-array, e.g. multiwell plates · CPC title

• G01N2021/6478

  Special lenses · CPC title

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title