Vortex generator for agitation of fluids during sample preparation

Patent metadata

Field	Value
Publication number	US-11680258-B2
Application number	US-202117759417-A
Country	US
Kind code	B2
Filing date	Feb 1, 2021
Priority date	Mar 13, 2020
Publication date	Jun 20, 2023
Grant date	Jun 20, 2023

Abstract

An apparatus, vortex generator assembly and method for automated cell lysis and nucleic acid purification and processing. The vortex generator assembly includes sample holder having a lysis well, at least one wash well, and an elution well. The vortex generator assembly also includes a sample holder cover having a plurality of vibration rods for creating a vortex in the wells of the sample holder. The apparatus includes motor operating a rotating cam to cause the vibration rods to vibrate and create the vortex in a well holding fluid and magnetic beads, wherein the vortexing speed is sufficient to overcome the magnetic attraction between the beads and disperse the beads in solution, to collect nucleic acids such as DNA.

First claim

We claim:

1. A sample lysis and nucleic acid extraction apparatus, comprising:
(i) a base for retaining a vortex generator assembly, the vortex generator assembly comprising:
a sample holder comprising a body member forming a channel extending from a first end to an opposite second end of the sample holder, the sample holder further comprising a first plurality of wells extending in a downwardly direction from a floor of the channel, each well having an interior volume; and
a sample holder cover comprising a base member comprising a floor extending from a first end to an opposite end of the sample holder cover and a second plurality of vibration rods extending in a downwardly direction from the floor of the base member into the interior volume of a corresponding one of the plurality of wells when the base member is inserted into the channel;

(ii) a rotating cam attached to a motor and configured to impart vibrational movement to one or more of the second plurality of vibration rods; and
(iii) a horizontal actuator attached to the motor and configured to selectively impart horizontal movement of the motor between adjacent rods.

2. The apparatus of claim 1 , further comprising a motor configured to
selectively move a magnet horizontally closer to and farther away from a first position adjacent to the vortex generator assembly and to selectively impart vertical movement of the magnet along a surface of a sample holder well.

3. The apparatus of claim 2 , wherein the motor is further configured to impart horizontal movement of the magnet along a surface of the sample holder between adjacent wells.

4. The apparatus of claim 3 , wherein the first plurality of wells further comprises a lysis well, at least one wash well, and an elution well.

5. The apparatus of claim 4 , further comprising a lysis well heating block configured to heat the lysis well of the vortex generator assembly.

6. The apparatus of claim 4 , further comprising an elution well heating block configured to heat the elution well of the vortex generator assembly.

7. A system for isolating nucleic acids from a biological sample, comprising the apparatus of claim 1 , a sample holder comprising a lysis well, at least one wash well, and an elution well and a sample holder cover comprising a plurality of rods inserted into at least one of the wells of the sample holder.

Assignees

• Siemens Healthcare Diagnostics Inc

Inventors

• Lee Alex Hofai
• Chu Daniel

Classifications

• B03C1/288

  disposed at the outer circumference of a recipient · CPC title

• B03C1/01

  by addition of magnetic adjuvants · CPC title

• B01F31/441

  performing a rectilinear reciprocating movement · CPC title

• B01F31/449

  Stirrers constructions · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title