Single pass tangential flow filtration systems and tangential flow filtration systems with recirculation of retentate

Patent metadata

Field	Value
Publication number	US-11679349-B2
Application number	US-202117315721-A
Country	US
Kind code	B2
Filing date	May 10, 2021
Priority date	Aug 29, 2014
Publication date	Jun 20, 2023
Grant date	Jun 20, 2023

Abstract

A method of filtering a liquid feed is described, comprising passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers. A method of filtering a liquid feed is also described comprising passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods of the invention can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.

First claim

What is claimed is:

1. A method of filtering a liquid feed, comprising:
a) passing a liquid feed through a tangential flow filtration (TFF) system that comprises:
a plurality of stacked filtration modules that are fluidly connected, each filtration module comprising:
a single manifold segment comprising a first manifold for receiving and carrying the feed into the filtration module, a second manifold for receiving and carrying retentate out of the filtration module, and a third manifold for receiving and carrying permeate through the filtration module; and
a plurality of TFF cassettes that are stacked on one or both faces of, and are fluidly connected to, the manifold segment, wherein the manifold segments are stacked and the manifolds in the manifold segments are coupled to create a centralized serial flow path through the system for feed and retentate;
a feed inlet on the first filtration module in the system;
a retentate outlet on the last filtration module in the system;
the TFF system having a liquid flow path that is parallel through the cassettes in each filtration module and serial through the manifold segments in adjacent filtration modules by coupling the first manifold in each manifold segment to the second manifold of an adjacent manifold segment, such that the retentate of one module serves as the feed for the manifold segment of the next filtration module, except that the first manifold in the first manifold segment of the filtration module in the system is connected to the feed inlet and the second manifold in the manifold segment of the last filtration module in the system is connected to the retentate outlet and wherein diverter plates are explicitly excluded;

b) recovering permeate in a permeate container and recovering a portion of the retentate from the system in a retentate container without recirculation of the recovered retentate through the TFF system.

2. The method of claim 1 , wherein about 10% or less of the retentate exiting the TFF system is recirculated.

3. The method of claim 1 , wherein the TFF system includes a valve or flow meter that is positioned on the retentate outlet or conduit carrying retentate from the system to the retentate container to control the amount of retentate that is recirculated.

4. The method of claim 1 , wherein retentate is recirculated back to a feed container in the TFF system or back to a feed line after a feed pump in the TFF system.

5. The method of claim 1 , wherein the first and second manifolds in the manifold segment contain a seal or a valve.

6. The method of claim 1 , wherein each filtration module contains one or more TFF cassettes on one or both faces of the manifold segment.

7. The method of claim 1 , wherein the filtration area for each filtration module is about 20 m 2 or less.

8. The method of claim 1 , wherein at least one filtration module further comprise valves on the flow channels between the TFF cassettes, wherein the cassettes are processed in parallel when the valves are open, or in series when the valves are closed.

9. The method of claim 1 , further comprising adding a diafiltration step, wherein the diafiltration step includes 10 concentration and dilution steps.

10. The method of claim 1 , wherein the TFF system further comprises one or more of: sampling port, a T-line for in-line buffer addition, a pressure sensor, a diaphragm for a pressure sensor, a reservoir for diafiltration solution, and a conduit for delivering diafiltration to a feed reservoir.

11. The method of claim 1 , wherein the filtration modules can have the same or different filtration areas from each other.

12. The method of claim 1 , wherein the manifolds in the single manifold segment have the same diameter throughout the filtration system or the manifolds in the single manifold segment have progressively smaller diameters from single manifold segment to single manifold segment.

13. The method of claim 7 , wherein the filtration area for each filtration module is selected from about 10 m 2 , about 1.0 m 2 or about 0.1 m 2 .

Assignees

• Emd Millipore Corp

Inventors

• Steen Jonathan
• Kozlov Mikhail
• Lutz Herbert
• Parrella Joseph
• Goodrich Elizabeth M
• Raghunath Bala

Classifications

• B01D63/082

  comprising a stack of flat membranes · CPC title

• B01D2315/16

  Diafiltration · CPC title

• B01D61/147

  Microfiltration · CPC title

• B01D61/145 Primary

  Ultrafiltration · CPC title

• B01D2313/105

  Supply manifolds · CPC title