System and method for cell levitation and monitoring
US-2016370386-A1 · Dec 22, 2016 · US
US11676750B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11676750-B2 |
| Application number | US-201917279419-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 26, 2019 |
| Priority date | Sep 28, 2018 |
| Publication date | Jun 13, 2023 |
| Grant date | Jun 13, 2023 |
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A magnetic levitation system is described, including a first cylinder-shaped magnet; a second cylinder-shaped magnet coaxially aligned with the first cylinder-shaped magnet; and a first cavity coaxially aligned with the first cylinder-shaped magnet; wherein the surfaces of the like-poles of the first and second cylinder-shaped magnets are parallel to each other and face each other to result in a linear magnetic field between the first and the second magnets. Methods of using a magnetic levitation system for analyzing a diamagnetic or paramagnetic sample are also described.
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The invention claimed is: 1. A magnetic levitation system comprising: a first cylinder-shaped magnet; a second cylinder-shaped magnet coaxially aligned with the first cylinder-shaped magnet; a first cavity coaxially aligned with and formed in the first cylinder-shaped magnet; and a container configured to hold a paramagnetic solution; wherein the surfaces of like polarity of the first and second cylinder-shaped magnets are parallel to each other and face each other to result in a linear, axially symmetric magnetic field between the first and the second magnets; and wherein the container is at least partially disposed between the first and the second cylinder-shaped magnets. 2. The system of claim 1 , wherein the first cavity is cylinder-shaped. 3. The system of claim 1 , wherein the first cavity spans through the entire height of the first cylinder-shaped magnet. 4. The system of claim 1 , wherein the linear, axially symmetric magnetic field extends into the first cavity. 5. The system of claim 1 , wherein the first and second cylinder-shaped magnets have a different radius or height. 6. The system of claim 1 , wherein the first and second cylinder-shaped magnets have the same radius, height or both. 7. The system of claim 1 , wherein the system further comprises a second cavity formed in and coaxially aligned with the second cylinder-shaped magnet. 8. The system of claim 7 , wherein the second cavity is cylinder-shaped. 9. The system of claim 1 , wherein the distance between the surfaces of like polarity of the first and second cylinder-shaped magnets is d and the heights of the first and second cylinder-shaped magnets are h 1 and h 2 , respectively; and the ratio of at least one of h 1 and h 2 to d is from about 0.2:1 to about 10:1. 10. The system of claim 9 , wherein the ratio of at least one of h 1 and h 2 to d is about 1.67:1. 11. The system of claim 9 , wherein the first cylinder-shaped magnet's inner diameter is id 1 ; and the ratio of id 1 to d is from about 0.2:1 to about 10:1. 12. The system of claim 11 , wherein the ratio of id 1 to d is about 1.67:1. 13. The system of claim 9 , wherein the first cylinder-shaped magnet's outer diameter is od 1 ; and the ratio of od 1 to d is from about 0.3:1 to about 100:1. 14. The system of claim 13 , wherein the ratio of od 1 to d is about 5:1. 15. The system of claim 9 , wherein the system further comprises a second cylinder-shaped cavity formed in and coaxially aligned with the second cylinder-shaped magnet; the second cylinder-shaped magnet's inner diameter is id 2 ; and the ratio of id 2 to d is from about 0.2:1 to about 10:1. 16. The system of claim 15 , wherein the ratio of id 2 to d is about 1.67:1. 17. The system of claim 9 , wherein the system further comprises a second cavity formed in and coaxially aligned with the second cylinder-shaped magnet; the second cylinder-shaped magnet's outer diameter is od 2 ; and the ratio of od 2 to d is from about 0.3:1 to about 100:1. 18. The system of claim 17 , wherein the ratio of od 2 to d is about 5:1. 19. The system of claim 1 , wherein the first cavity is cylinder-shaped and the relationship of the first cylinder-shaped magnet's inner diameter (id 1 ):outer diameter (od 1 ):height (h 1 ):distance between the surfaces of like polarity of the first and second cylinder-shaped magnets (d) is about 1.67:5:1.67:1. 20. The system of claim 1 , wherein the system further comprises a second cylinder-shaped cavity formed in and coaxially aligned with the second cylinder-shaped magnet; and the relationship of the second cylinder-shaped magnet's inner diameter (id 2 ):outer diameter (od 2 ):height (h 2 ):distance between the surfaces of like polarity of the first and second cylinder-shaped magnets (d) is about 1.67:5:1.67:1. 21. The system of claim 1 , wherein the container further comprises an inlet configured to allow adding or removing the paramagnetic solution or a paramagnetic or diamagnetic sample. 22. The system of claim 1 , wherein the container comprises two ends, at least one of which extends into or through the first cavity. 23. The system of claim 22 , wherein the system further comprises a second cavity formed in and coaxially aligned with the second cylinder-shaped magnet; and the other end of the container's two ends extends into or through the second cylinder-shaped cavity. 24. The system of claim 1 , wherein the container is a cuvette. 25. The system of claim 1 , wherein the paramagnetic solution comprises an aqueous solution of a paramagnetic compound. 26. The system of claim 25 , wherein the paramagnetic compound is selected from the group consisting of MnCl 2 , MnBr 2 , CuSO 4 , GdCl 3 , DyCl 3 , HoCl 3 , a Gd chelated compound, and a combination thereof. 27. The system of claim 26 , wherein the Gd chelated compound is gadolinium (III) diethylenetriaminepentaacetic acid. 28. The system of claim 1 , wherein the paramagnetic solution comprises a MnCl 2 aqueous solution. 29. The system of claim 1 , wherein the paramagnetic solution comprises a hydrophobic paramagnetic solution or a paramagnetic ionic liquid. 30. The system of claim 1 , wherein the maximal magnetic field along the common axis of the first and second cylinder-shaped magnets is from about 0.20 T to about 0.05 T. 31. The system of claim 1 , wherein the first and second cylinder-shaped magnets are NdFeB magnets. 32. The system of claim 1 , wherein the system optionally comprises a second cylinder-shaped cavity spanning through the entire height of the second cylinder-shaped magnet and coaxially aligned with the second cylinder-shaped magnet; and the first or second cylinder-shaped magnet's inner diameter is from about 15 mm to about 40 mm. 33. The system of claim 32 , wherein the first or second cylinder-shaped magnet's inner diameter is about 25 mm. 34. The system of claim 1 , wherein the system optionally comprises a second cylinder-shaped cavity spanning through the entire height of the second cylinder-shaped magnet and coaxially aligned with the second cylinder-shaped magnet; and the first or second cylinder-shaped magnet's outer diameter is from about 50 mm to about 100 mm. 35. The system of claim 34 , wherein the first or second cylinder-shaped magnet's outer diameter is about 76 mm. 36. The system of claim 1 , wherein the first or second cylinder-shaped magnet's height is from about 15 mm to about 50 mm. 37. The system of claim 36 , wherein the first or second cylinder-shaped magnet's height is about 25 mm. 38. The system of claim 1 , wherein the distance between the surfaces of like polarity of the first and second cylinder-shaped magnets is from about 5 mm to about 50 mm. 39. The system of claim 38 , wherein the distance between the surfaces of like polarity of the first and second cylinder-shaped magnets is about 15 mm. 40. A method of analyzing a diamagnetic or paramagnetic sample, comprising: providing the system of claim 1 ; adding a paramagnetic solution and a diamagnetic or paramagnetic sample, either separately or together, into the container; and allowing the diamagnetic or paramagnetic s
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