PIRNA-54265 detection kit used for early screening, diagnosis, curative efficacy monitoring and prognostic evaluation of colorectal cancer

Patent metadata

Field	Value
Publication number	US-11674172-B2
Application number	US-202217830354-A
Country	US
Kind code	B2
Filing date	Jun 2, 2022
Priority date	Feb 12, 2018
Publication date	Jun 13, 2023
Grant date	Jun 13, 2023

Abstract

The present invention relates to a piRNA-54265 detection kit used for early screening, diagnosis, efficacy monitoring and/or prognostic evaluation of colorectal cancer. The detection kit includes a primers combination, including a primer pair and a probe for specifically detecting piRNA-54265; the primer pair is a piRNA-54265 stem-loop PCR primer pair, or a piRNA-54265 PolyA tailed PCR primer pair; the primer pair includes a forward primer and a reverse primer.

First claim

What is claimed is:

1. A composition comprising a primer pair and probe for specifically detecting piRNA-54265, wherein said primer pair is selected from:
(a) a first primer pair for amplifying piRNA-54265 containing a stem loop comprising a forward primer consisting of the nucleotide sequence of SEQ ID NO: 26 and a reverse primer consisting of the nucleotide sequence of SEQ ID NO: 9 and
(b) a second primer pair for amplifying a poly-A tailed piRNA-54265 comprising a forward primer consisting of the nucleotide sequence of SEQ ID NO: 26 and a reverse primer consisting of the nucleotide sequence of SEQ ID NO: 22; and
wherein said probe consists of the nucleotide sequence of SEQ ID NO: 27.

2. The composition according to claim 1 , further comprising a primer for reverse transcription.

3. The composition according to claim 2 , wherein the primer for reverse transcription is for reverse transcribing a piRNA-54265 containing a stem loop and wherein the primer for reverse transcription consists of the nucleotide sequence of SEQ ID NO: 2.

4. A piRNA-54265 detection kit, comprising the composition according to claim 3 .

5. The composition according to claim 2 , wherein the primer for reverse transcription is for reverse transcribing a poly-A tailed piRNA-54265 and wherein the primer for reverse transcription consists of one of the nucleotide sequences selected from SEQ ID NOs: 3-5.

6. A piRNA-54265 detection kit, comprising the composition according to claim 5 .

7. A piRNA-54265 detection kit, comprising the composition according to claim 2 .

8. A piRNA-54265 detection kit, comprising the composition according to claim 1 .

Assignees

• Univ Sun Yat Sen
• Sun Yat Sen Univ Cancer Center

Inventors

• Lin Dongxin
• Zheng Jian
• Mai Dongmei
• Tan Liping

Classifications

• C12N15/113

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title

• A61K31/7105

  Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links · CPC title

• C12Q1/6886 Primary

  for cancer (immunoassay for cancer G01N33/575) · CPC title

• C12Q1/6827 Primary

  for detection of mutation or polymorphism · CPC title

• C12N2310/113

  targeting other non-coding nucleic acids, e.g. antagomirs · CPC title