Genetically modified pig cells with an inactivated Etv2 gene

Patent metadata

Field	Value
Publication number	US-11673928-B2
Application number	US-201615554585-A
Country	US
Kind code	B2
Filing date	Mar 3, 2016
Priority date	Mar 3, 2015
Publication date	Jun 13, 2023
Grant date	Jun 13, 2023

Abstract

Described herein is a method for producing a chimeric non-human animal expressing a human ETV2 gene comprising: a) generating an ETV2 null non-human animal cell, wherein both copies of the non-human ETV2 gene carry a mutation that prevents production of functional ETV2 protein in said non-human animal; b) creating an ETV2 null non-human blastocyst by somatic cell nuclear transfer comprising fusing a nucleus from said ETV2 null non-human animal cell of a) into an enucleated non-human oocyte and activating said oocyte to divide so as to form an ETV2 null non-human blastocyst; c) introducing human stem cells into the ETV2 null non-human blastocyst of b); and d) implanting said blastocyst from c) into a pseudopregnant surrogate non-human animal to generate a chimeric non-human animal expressing human ETV2.

First claim

What is claimed is:

1. A genetically modified isolated Sus scrofa pig cell, morula or blastocyst whose genome comprises a homozygous deletion of an endogenous ETV2 gene, wherein the cell, morula or blastocyst lacks functional endogenous ETV2 protein.

Assignees

• Univ Minnesota

Inventors

• Garry Daniel J
• Garry Mary G
• Rasmussen Tara
• Koyano Naoko

Classifications

• A01K67/0271 Primary

  Chimeric vertebrates, e.g. comprising exogenous cells · CPC title

• A01K2207/15

  Humanized animals · CPC title

• C12N15/8778

  Swine embryos · CPC title

• A01K2217/075

  inducing loss of function, i.e. knock out · CPC title

• C07K14/4705 Primary

  stimulating, promoting or activating activity · CPC title