Nanopore devices for sensing biomolecules

Patent metadata

Field	Value
Publication number	US-11673136-B2
Application number	US-201815945717-A
Country	US
Kind code	B2
Filing date	Apr 4, 2018
Priority date	Apr 4, 2017
Publication date	Jun 13, 2023
Grant date	Jun 13, 2023

Abstract

The present disclosure provides chips, devices and methods for sequencing a biomolecule. The biomolecule may be DNA, RNA. a protein, or a peptide. The chip comprises a substrate; a first and second fluid chamber; fluid channels connecting the first and second fluid chamber; a first and second electrode disposed on opposing sides of the central fluid channel and having a nanogap therebetween, wherein the width of the nanogap is modulated by confined electrochemical deposition; and a passivation layer disposed on top of the first and second electrodes and the fluid channel.

First claim

What is claimed is:

1. An assay chip comprising
(a) a substrate;
(b) a first and second fluid chamber;
(c) a first fluid channel connecting the first and second fluid chamber, the first fluid channel being planar with the substrate;
(d) a first and second electrode disposed on a top surface of the substrate and in the first fluid channel, located between the first and second fluid chamber, and having a nanogap therebetween, wherein the width of the nanogap is configured to be modulated by a confined electrochemical deposition process; and
(e) a passivation layer disposed on respective top surfaces of the first and second electrodes and on top of the first fluid channel such that the nanogap is between the substrate and the passivation layer,
wherein the passivation layer directly contacts the respective top surfaces of the first and second electrodes at the nanogap and extends over the nanogap between the respective top surfaces of first and second electrodes to confine a top of the nanogap.

2. The assay chip of claim 1 , further comprising
(f) a second and third fluid channel, each having a plurality of translocation guidance canals, wherein the second fluid channel connects the first fluid chamber to the first fluid channel and the third fluid channel connects the second fluid chamber to the first fluid channel; and
wherein the passivation layer is disposed on top of the first and second electrodes and the first, second, and third fluid channels.

3. The chip of claim 2 , wherein the nanogap has a width of about 1 nm to about 20 nm.

4. The chip of claim 2 , wherein the substrate is a coated glass substrate.

5. The chip of claim 2 , wherein the first and second fluid chamber comprise a material selected from the group consisting of polydimethylsiloxane, epoxy, silica and combinations thereof.

6. The chip of claim 2 , wherein the first and second electrode comprise gold, platinum or palladium.

7. The chip of claim 2 , wherein the passivation layer comprises a material selected from the group consisting of silicon dioxide, silicon nitride, hafnium oxide, zirconium dioxide, aluminum oxide, titanium oxide, SU-8 polymer, and combinations thereof.

8. The chip of claim 2 , wherein a first reagent is attached to the first electrode and a second reagent is attached to the second electrode, wherein the first and the second reagent are capable of interacting with a biomolecule.

9. The chip of claim 1 , wherein the chip is configured to modulate the width of the nanogap by applying a reversible bias between the first and second electrodes and a counter electrode.

Assignees

• Qing Quan
• Wang Yuan
• Sadar Joshua
• Univ Arizona State

Inventors

• Qing Quan
• Wang Yuan
• Sadar Joshua

Classifications

• G01N27/3278

  involving nanosized elements, e.g. nanogaps or nanoparticles (nanopores G01N33/48721; magnetic beads G01N27/745) · CPC title

• C12Q1/6869 Primary

  Methods for sequencing · CPC title

• G01N27/3276

  being a hybridisation with immobilised receptors (using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68) · CPC title

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title

• B01L3/502761

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title