Microfluidic device and detection method thereof

Patent metadata

Field	Value
Publication number	US-11660597-B2
Application number	US-202117231842-A
Country	US
Kind code	B2
Filing date	Apr 15, 2021
Priority date	Feb 1, 2021
Publication date	May 30, 2023
Grant date	May 30, 2023

Abstract

A microfluidic device includes: first substrate, microfluidic channel layer, and second substrate; the first substrate includes light source layer including a plurality of light source structures, the light source structure includes first electrode, second electrode, and an electroluminescence module, and when being turned on, emits light passing through the microfluidic channel layer and irradiating the second substrate; the second substrate includes photoelectric detection layer including a plurality of photoelectric detection structures and driving electrode layer including a plurality of driving electrodes and a plurality of driving circuits, the photoelectric detection structure includes third electrode, fourth electrode, and photoelectric conversion module arranged therebetween, and when being turned on, generates an electrical signal according to an incident light signal; the driving circuit is configured to apply a voltage to each driving electrode such that a droplet moves in a microfluidic channel of the microfluidic channel layer.

First claim

What is claimed is:

1. A microfluidic device, comprising:
a first substrate and a second substrate that are disposed oppositely,
and a microfluidic channel layer arranged between the first substrate and the second substrate,
wherein the first substrate comprises a light source layer, the light source layer comprises a plurality of light source structures, the light source structure comprises a first electrode, a second electrode, and an electroluminescence module arranged between the first electrode and the second electrode, and in a case where the light source structure is turned on, the light source structure is configured to emit light passing through the microfluidic channel layer and irradiating the second substrate;
the second substrate comprises a photoelectric detection layer and a driving electrode layer, the photoelectric detection layer comprises a plurality of photoelectric detection structures, the photoelectric detection structure comprises a third electrode, a fourth electrode, and a photoelectric conversion module arranged between the third electrode and the fourth electrode, and the photoelectric detection structure generates an electrical signal according to an incident light signal in a case where the photoelectric detection structure is turned on; and
the driving electrode layer comprises a plurality of driving electrodes and a plurality of driving circuits, and the driving circuit is configured to apply a voltage to the driving electrode such that a droplet moves in a microfluidic channel of the microfluidic channel layer;
wherein one of the light source structures is disposed corresponding to one of the photoelectric detection structures; and
in a direction perpendicular to the second substrate, an orthographic projection of the electroluminescence module overlaps with an orthographic projection of the photoelectric conversion module; and
wherein the third electrode is arranged between the fourth electrode and the microfluidic channel layer, and the third electrode is multiplexed as the driving electrode, wherein the driving circuit is configured to control the photoelectric detection structures to be turn on at different times to apply a voltage to the third electrode such that the droplet moves in the microfluidic channel.

2. The microfluidic device of claim 1 , wherein the first electrode and the second electrode are arranged in a same layer and insulated, the electroluminescence module is arranged between the first electrode and the microfluidic channel layer, and the electroluminescence module comprises a micro light-emitting diode.

3. The microfluidic device of claim 1 , wherein the first electrode and the second electrode are arranged in a same layer and insulated, the electroluminescence module is arranged between the first electrode and the second electrode, and the electroluminescence module comprises an organic luminescent material.

4. The microfluidic device of claim 1 , wherein in a case where the light source structure is turned on, the light source structure of the light source layer is configured to emit light in a first wavelength band; or
the light source layer comprises at least a first light source structure and a second light source structure, and the first light source structure is configured to emit light in a first wavelength band and the second light source structure is configured to emit light in a second wavelength band.

5. The microfluidic device of claim 1 , wherein the photoelectric detection structure further comprises a first switch device;
the second substrate further comprises a plurality of first source lines and a plurality of first gate lines that are insulated from each other; and
a control end of the first switch device is electrically connected to the first gate line, a first end of the first switch device is electrically connected to the first source line, and a second end of the first switch device is electrically connected to the third electrode.

6. The microfluidic device of claim 5 , wherein the second substrate further comprises a bias voltage line; and each the fourth electrode is electrically connected to the bias voltage line.

7. The microfluidic device of claim 1 , wherein the driving electrode layer is arranged between the photoelectric detection layer and the microfluidic channel layer, wherein one of the driving electrodes is disposed corresponding to one of the light source structures; and
in the direction perpendicular to the second substrate, an orthographic projection of the driving electrode overlaps with the orthographic projection of the electroluminescence module;
or,
wherein one of the driving electrodes is disposed corresponding to m light source structures, wherein m is a positive integer greater than 1; and
in the direction perpendicular to the second substrate, an orthographic projection of the driving electrode overlaps with orthographic projections of m electroluminescence modules.

8. The microfluidic device of claim 7 , wherein the driving electrode layer comprises a plurality of driving electrode units, and the driving electrode unit comprises n driving electrodes, wherein n is a positive integer greater than 1; and
the driving circuit is configured to control a same voltage to be applied to each the driving electrode in the driving electrode unit, and control a voltage difference between voltages to be applied to two adjacent driving electrode units to be greater than or equal to a droplet movement threshold voltage, such that the droplet moves in the microfluidic channel,
wherein in the direction perpendicular to the second substrate, the orthographic projection of the driving electrode unit covers orthographic projections of a plurality of electroluminescence modules of the light source structure.

9. The microfluidic device of claim 7 , wherein the light source layer comprises a light source control circuit, and the light source control circuit is configured to control the light source structures to be turned on at a same time; and
the photoelectric detection structures of the photoelectric detection layer are configured to be turned on at different times and output electrical signals for droplet positioning;
wherein the light source control circuit is configured to control light source structures in an area where the droplet is located, light source structures that emit lights in a same wavelength band are turned on at a same time and light source structures that emit lights in different wavelength bands are turned on at different times; and
the photoelectric detection structures in the area where the droplet is located are configured to be turned on at different times and output electrical signals for spectral detection.

10. The microfluidic device of claim 1 , wherein the photoelectric detection layer comprises a plurality of photoelectric detection units, and the photoelectric detection unit comprises n photoelectric detection structures, wherein n is a positive integer greater than 1; and
the driving circuit is configured to control the n photoelectric detection structures in the photoelectric detection unit to be turned on at a same time to apply a same voltage to the third electrode, and the driving circuit is further configured to control a voltage difference between voltages to be applied to two adjacent photoelectric detection units, the voltage difference is greater than or equal to a droplet movement threshold voltage, such that the droplet moves in the microfluidic channel.

11. The microfluidic device of claim 10 , wherein the light source layer comprises a light source control circuit, and the light source control circuit is configured to control the light source structures

Assignees

• Shanghai Avic Opto Electronics Co Ltd

Inventors

• Lin Baiquan
• Xi Kerui
• Jia Zhenyu
• Ouyang Junting
• Qin Feng
• Peng Xuhui

Classifications

• B01L3/502792

  for moving individual droplets on a plate, e.g. by locally altering surface tension · CPC title

• G01N21/59

  Transmissivity (G01N21/25 takes precedence) · CPC title

• G01N21/255 Primary

  Details, e.g. use of specially adapted sources, lighting or optical systems · CPC title

• B01L3/5027 Primary

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title

• G01N21/01

  Arrangements or apparatus for facilitating the optical investigation · CPC title