Herbicide tolerance genes and methods of use thereof

Patent metadata

Field	Value
Publication number	US-11655480-B2
Application number	US-202117150604-A
Country	US
Kind code	B2
Filing date	Jan 15, 2021
Priority date	Oct 15, 2014
Publication date	May 23, 2023
Grant date	May 23, 2023

Abstract

Polypeptides and recombinant DNA molecules useful for conferring tolerance to AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides are provided in the present invention, as well as herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.

First claim

What is claimed is:

1. A recombinant DNA molecule comprising a nucleic acid sequence encoding a polypeptide having at least 90% identity to the amino acid sequence of SEQ ID NO:51, wherein the polypeptide confers herbicide tolerance to a transgenic plant.

2. The recombinant DNA molecule of claim 1 , wherein the nucleic acid sequence comprises SEQ ID NO:58.

3. The recombinant DNA molecule of claim 1 , wherein the recombinant DNA molecule is operably linked to a heterologous promoter functional in a plant cell.

4. The recombinant DNA molecule of claim 3 , wherein the recombinant DNA molecule is further operably linked to a DNA molecule encoding a chloroplast transit peptide.

5. A DNA construct comprising a heterologous promoter functional in a plant cell operably linked to the recombinant DNA molecule of claim 1 .

6. The DNA construct of claim 5 , further comprising a DNA molecule encoding a chloroplast transit peptide operably linked to the recombinant DNA molecule.

7. The DNA construct of claim 5 , wherein the nucleic acid sequence encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:51.

8. The DNA construct of claim 5 , wherein the DNA construct is present in the genome of a transgenic plant.

9. A plant, seed, plant tissue, plant part, or cell comprising a recombinant DNA molecule comprising a nucleic acid sequence encoding a polypeptide having at least 90% identity to the amino acid sequence of SEQ ID NO:51.

10. The plant, seed, plant tissue, plant part, or cell of claim 9 , wherein the plant, seed, plant tissue, plant part, or cell comprises tolerance to at least one herbicide selected from the group consisting of AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides.

11. A plant, seed, plant tissue, plant part, or cell comprising the DNA construct of claim 5 .

12. A plant, seed, plant tissue, plant part, or cell comprising the polypeptide encoded by the recombinant DNA molecule of claim 1 .

13. A polypeptide having at least 90% identity to the amino acid sequence of SEQ ID NO:51, wherein the polypeptide confers herbicide tolerance to a transgenic plant.

14. The polypeptide of claim 13 , wherein the polypeptide has oxygenase activity against at least one herbicide selected from the group consisting of AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides.

15. A method for conferring herbicide tolerance to a plant, seed, cell, or plant part comprising expressing in the plant, seed, cell, or plant part the polypeptide of claim 13 .

16. The method of claim 15 , wherein the plant, seed, cell, or plant part comprises a DNA construct comprising a heterologous promoter functional in a plant cell operably linked to a recombinant DNA molecule comprising a nucleic acid sequence encoding a polypeptide having at least 90% identity to the amino acid sequence of SEQ ID NO:51.

17. The method of claim 15 , wherein the plant, seed, cell, or plant part comprises tolerance to at least one herbicide selected from the group consisting of AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides.

18. A method for producing an herbicide tolerant transgenic plant comprising transforming a plant cell or tissue with a recombinant DNA molecule comprising a nucleic acid sequence selected from the group consisting of:
a) a nucleic acid sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 51;
b) a nucleic acid sequence comprising SEQ ID NO:58; and
c) a nucleic acid sequence encoding a polypeptide having at least 90% identity to the amino acid sequence of SEQ ID NO: 51, and capable of conferring herbicide tolerance to a transgenic plant;
and regenerating an herbicide tolerant transgenic plant from the transformed plant cell or tissue.

19. The method of claim 18 , wherein said herbicide tolerant transgenic plant comprises tolerance to at least one herbicide selected from the group consisting of AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides.

20. A method for controlling weeds in a plant growth area, comprising contacting a plant growth area comprising a plant or seed comprising a recombinant DNA molecule comprising a nucleic acid sequence encoding a protein having at least 90% identity to the amino acid sequence of SEQ ID NO:51 with at least one herbicide selected from the group consisting of AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides, wherein the plant or seed is tolerant to the at least one herbicide.

21. The recombinant DNA molecule of claim 1 , wherein the polypeptide has at least 91% sequence identity to the amino acid sequence of SEQ ID NO: 51.

22. The plant, seed, plant tissue, plant part, or cell of claim 9 , wherein the polypeptide has at least 91% sequence identity to the amino acid sequence of SEQ ID NO: 51.

23. The method of claim 15 , wherein the polypeptide has at least 91% sequence identity to the amino acid sequence of SEQ ID NO: 51.

Assignees

• Monsanto Technology Llc

Inventors

• Ellis Christine M
• Evdokimov Artem G
• Feng Paul C C
• Fu Xiaoran
• Larue Clayton T
• Nageotte Jeffrey R
• Read Andrew C
• Shi Lei
• Wollacott Andrew M

Classifications

• A01H5/10 Primary

  Seeds · CPC title

• A01N57/00

  Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds · CPC title

• C07K2319/08

  containing a chloroplast localisation signal · CPC title

• C12Y114/11033

  DNA oxidative demethylase (1.14.11.33) · CPC title

• C12N15/8274 Primary

  for herbicide resistance · CPC title