Substrates coated with selective cell separation or cell culture polymers

Patent metadata

Field	Value
Publication number	US-11655273-B2
Application number	US-201816629494-A
Country	US
Kind code	B2
Filing date	Jul 9, 2018
Priority date	Jul 11, 2017
Publication date	May 23, 2023
Grant date	May 23, 2023

Abstract

Provided are a ligand-bearing substrate which has a surface at least partially coated with a polymer (P3) containing structural units represented by the formulae (1 a ) and (1 b ) (in the formulae, R 1 , R 2 , X, Y, L, Q 1 , Q 2 , Q 3 , m1, m2 and n are as described in the claims and description); a raw material for such a substrate; and a method for producing such substrates.

First claim

The invention claimed is:

1. A ligand-bearing substrate having a surface at least partially coated with a polymer (P3) comprising structural units represented by the following formulae (1a) and (1b):

wherein R 1 and R 2 each independently denote a C1-C5 linear alkyl group, X denotes a hydrogen atom or a C1-C5 linear alkyl group, Y denotes a carboanion (—COO − group) or a sulfoanion (—SO 3 − group), L denotes a ligand, Q 1 and Q 2 each independently denote an ester bond, an amide bond, a C1-C10 linear or branched alkylene group or an optionally substituted phenylene group, or a divalent group composed of a combination of any of the above bonds and groups, Q 3 denotes a divalent organic group containing a 1,2,3-triazole ring skeleton, m1 and m2 each independently denote an integer of 1 to 200, and n independently at each occurrence denotes an integer of 1 to 10.

2. The ligand-bearing substrate according to claim 1 , wherein the ligand is selected from the group consisting of peptides, antibodies, proteins and low-molecular compounds.

3. The ligand-bearing substrate according to claim 2 , wherein the peptide comprises Gln-Gln-Gly-Trp-Phe sequence.

4. The substrate according to claim 1 , which is a cell separation substrate.

5. The substrate according to claim 1 , which is a cell culture substrate.

6. The substrate according to claim 1 , wherein the substrate is a dish, a plate, a porous film, a particle or a filter.

7. The substrate according to claim 4 , which is capable of allowing at least one type of cells to be selectively separated from a cell mixture fluid containing at least two or more types of cells.

8. A cell separation method using the substrate described in claim 1 .

9. The substrate according to claim 5 , which is capable of allowing at least one type of cells to be selectively cultured from a cell mixture fluid containing at least two or more types of cells.

10. A cell culture method using the substrate described in claim 1 .

11. The ligand-bearing substrate according to claim 1 , wherein the polymer (P3) further comprises a structural unit represented by the following formula (1d):

wherein R 3 independently at each occurrence denotes a C1-C5 linear alkyl group, X denotes a hydrogen atom or a C1-C5 linear alkyl group, Q 4 denotes an ester bond, a phosphodiester bond, an amide bond, a C1-C10 linear or branched alkylene group or an optionally substituted phenylene group, or a divalent group composed of a combination of any of the above bonds and groups, and m3 denotes an integer of 1 to 200.

Assignees

• National Univ Corporation Univ Of Toyama
• Nissan Chemical Corp

Inventors

• Kitano Hiromi
• Nakaji Tadashi
• Usui Yuki
• Nishino Taito
• Kishioka Takahiro

Classifications

• C08F220/365

  containing further carboxylic moieties · CPC title

• B01J20/3285

  Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic · CPC title

• C08F220/40

  Esters of unsaturated alcohols {, e.g. allyl (meth)acrylate} · CPC title

• C12M23/20

  Material Coatings (immunocoatings C12M25/00) · CPC title

• C12N2533/30

  Synthetic polymers · CPC title