Encapsulation of active substances and/or micro-organisms in a lamellar material

Patent metadata

Field	Value
Publication number	US-11649196-B2
Application number	US-201916964122-A
Country	US
Kind code	B2
Filing date	Jan 22, 2019
Priority date	Jan 23, 2018
Publication date	May 16, 2023
Grant date	May 16, 2023

Abstract

The present invention relates to a method for encapsulating a compound selected from the group consisting of at least one active substance, at least one microorganism and mixtures thereof in an organic-inorganic hybrid material of 2:1 lamellar structure, said material having the following general formula I:Nax[(Mg3)(Alx(RSi)4−x)O8+x(OH)2]  (I)the method comprising:a) sol-gel synthesis of the organic-inorganic hybrid material of 2:1 lamellar structure in the presence of the compound;b) recovery of the compound encapsulated in the material of general formula I.It further relates to the compound encapsulated in an organic-inorganic hybrid material of 2:1 lamellar structure of general formula I, a composition comprising same and its use for fertilizing, feeding, stimulating growth and/or prophylaxis of plants and/or improvement of the physical, chemical and/or biological properties of the soil or of the culture substrate of plants.

First claim

The invention claimed is:

1. A method of encapsulating a compound selected from the group consisting of at least one active substance, at least one microorganism and mixtures thereof in an organic-inorganic hybrid material of 2:1 lamellar structure, said organic-inorganic hybrid material having the following general formula I:
Na x [(Mg 3 )(Al x (RSi) 4−x )O 8+x (OH) 2 ]  (I)

in which
x is a number such that 0≤x<1.2 and
R represents a C 1 -C 30 alkyl group, an aryl group, a (C 1 -C 30 alkyl)aryl group or a C 1 -C 30 O-alkyl group, and the alkyl group may be substituted with a group selected from a phenyl, vinyl, or mercaptopropyl group;
the method comprising:
a) sol-gel synthesis of the organic-inorganic hybrid material of 2:1 lamellar structure in the presence of the compound; and
b) recovery of the compound encapsulated in the material of general formula I,
wherein the source of silicon necessary for synthesis of the material of formula I of step a) is an organoalkoxysilane or a mixture of organoalkoxysilanes of the following general formula II:
RSi(OR′) 3   (II)

in which
R′ is a methoxy or ethoxy group.

2. The method as claimed in claim 1 , wherein the encapsulated compound is a microorganism and step a) comprises the following successive steps:
a1) adding a source of magnesium, the microorganism, a solvent, a source of silicon and in the case when x≠0, the source of aluminum;
a2) adjusting the pH to between 8 and 14;
a3) stirring the mixture so as to obtain a gel.

3. The method as claimed in claim 2 , wherein it comprises the following successive steps after step a3):
a4) recovering the solid phase of the gel obtained in step a3);
a5) drying the solid phase of the gel obtained in step a4).

4. The method as claimed in claim 3 , wherein the drying step a5) consists of lyophilization.

5. The method as claimed in claim 2 , wherein the microorganism is in the form of a preculture of said microorganism and said method comprises a preliminary step before step a) of preparing the preculture of microorganism.

6. The method as claimed in claim 1 , wherein the source of silicon is selected from the group consisting of phenyltrimethoxysilane of the following formula (a): phenyl-Si(OCH 3 ) 3 (a), tetraethylorthosilicate of the following formula (b): Si(OC 2 H 5 ) 4 (b), hexadecyltrimethoxysilane of the following formula (c): CH 3 (CH 2 ) 14 CH 2 —Si(OCH 3 ) 3 (c) and mixtures thereof.

7. The method as claimed in claim 6 , wherein the encapsulated compound is an active substance and the source of silicon is a mixture of phenyltrimethoxysilane (a) and tetraethylorthosilicate (b).

8. The method as claimed in claim 6 , wherein the encapsulated compound is a microorganism and the source of silicon is selected from the group consisting of phenyltrimethoxysilane (a) and hexadecyltrimethoxysilane (c).

9. The method as claimed in claim 1 , wherein x=0.

10. The method as claimed in claim 1 , wherein the compound is an active substance selected from the group consisting of an amino acid, an essential oil, a vitamin and mixtures thereof.

11. The method as claimed in claim 1 , wherein the compound is a microorganism selected from the group consisting of a bacterium, a microalga, a fungus, and mixtures thereof.

12. The method as claimed in claim 1 , wherein the encapsulated compound is an active substance and step a) comprises the following successive steps:
a1) adding a source of magnesium, the active substance, the source of silicon, in the case when x≠0, the source of aluminum, and an optional solvent;
a2) adjusting the pH to between 8 and 14;
a3) stirring the mixture, so as to obtain a gel;
a4) recovering the solid phase of the gel obtained in step a3);
a5) drying the solid phase of the gel obtained in step a4).

13. The method as claimed in claim 1 wherein the source of silicon is selected from the group consisting of phenyltrimethoxysilane (a), methyltriethoxysilane (c) and a mixture of phenyltrimethoxysilane (a) with tetraethylorthosilicate (b).

14. A compound encapsulated in an organic-inorganic hybrid material of 2:1 lamellar structure, said organic-inorganic hybrid material having the following general formula I:
Na x [(Mg 3 )(Al x (RSi) 4−x )O 8+x (OH) 2 ]  (I)

in which
x is a number such that 0≤x<1.2 and
R represents a C 1 -C 30 alkyl group, an aryl group, a (C 1 -C 30 alkyl)aryl group or a C 1 -C 30 O-alkyl group, and the alkyl group may be substituted with a group selected from a phenyl, vinyl, or mercaptopropyl group;
the encapsulated compound being selected from the group consisting of at least one active substance, at least one microorganism and mixtures thereof.

15. A composition comprising the compound encapsulated in an organic-inorganic hybrid material of 2:1 lamellar structure as claimed in claim 14 and an excipient.

16. The composition as claimed in claim 15 , which is in solid form, in liquid form or in the form of gel.

17. The composition as claimed in claim 15 , which further comprises nutrients, organic raw materials and/or mineral raw materials.

18. The compound encapsulated in an organic-inorganic hybrid material of 2:1 lamellar structure as claimed in claim 14 , which is obtained by a method comprising:
a) sol-gel synthesis of the organic-inorganic hybrid material of 2:1 lamellar structure in the presence of the compound; and
b) recovery of the compound encapsulated in the material of general formula I,
wherein the source of silicon necessary for synthesis of the material of formula I of step a) is an organoalkoxysilane or a mixture of organoalkoxysilanes of the following general formula II:
RSi(OR′) 3   (II)

in which
R′ is a methoxy or ethoxy group.

19. A method for fertilizing, feeding, stimulating growth and/or prophylaxis of plants and/or improving the physical, chemical and/or biological properties of the soil or of the culture substrate of plants comprising administration of an effective amount of a compound as claimed in claim 14 , or of a composition comprising the compound encapsulated in an organic-inorganic hybrid material of 2:1 lamellar structure as claimed in claim 14 and a suitable excipient, to a plant in need thereof or to the soil or culture substrate of a plant in need thereof.

20. The method as claimed in claim 19 , wherein the administration is an application to the leaves, to the roots, in the open or outside the soil of the plant in need thereof.

Assignees

• Agro Innovation Int
• Univ Haute Alsace
• Centre Nat Rech Scient

Inventors

• Pluchon Sylvain K
• Yvin Jean-Claude
• Brendle Jocelyne
• Limousy Lionel
• Dutournie Patrick
• Maillard Anne
• Bruneau Marion
• Bennici Simona

Classifications

• C05G3/80

  Soil conditioners · CPC title

• C05F11/08

  Organic fertilisers containing added bacterial cultures, mycelia or the like · CPC title

• C05F11/10

  Fertilisers containing plant vitamins or hormones · CPC title

• C05G3/60

  Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants · CPC title

• C01B33/22 Primary

  Magnesium silicates · CPC title