Method of reading the result of an electrophoretic assay comprising a digital image indicating the intensity of light emitted by chemiluminescence from the output medium of the electrophoretic assay

Patent metadata

Field	Value
Publication number	US-11644359-B2
Application number	US-202117346873-A
Country	US
Kind code	B2
Filing date	Jun 14, 2021
Priority date	Dec 15, 2017
Publication date	May 9, 2023
Grant date	May 9, 2023

Abstract

A combination imaging system includes a housing having a base and a lid, the lid having a closed position against the base and having an open position. The imaging device further includes a contact area image sensor. The lid shields the contact area image sensor from ambient light when the lid is in the closed position. The imaging device also includes a camera. The camera includes a lens, and the field of view of the camera encompasses at least a portion of an imaging area of the contact area image sensor when the lid is in the open position. The device may be especially useful for capturing a chemiluminescent image of an electrophoretic assay result, and capturing a colorimetric image of the same result, so that non-chemiluminescent protein standards may be located with respect to chemiluminescent analytes of interest.

First claim

What is claimed is:

1. A combination imaging system, comprising:
a housing having a base and a lid, the lid having a closed position against the base and having an open position;
a contact area image sensor, wherein the lid shields the contact area image sensor from ambient light when the lid is in the closed position;
a camera comprising a lens, wherein the field of view of the camera encompasses at least a portion of an imaging area of the contact area image sensor when the lid is in the open position; and
a controller configured to:
capture a first digital image using the contact area image sensor;
capture a second digital image using the camera; and
combine the first and second digital images into a composite image.

2. The combination imaging system of claim 1 , wherein the camera is mounted in the lid.

3. The combination imaging system of claim 1 , further comprising a light source controlled by the controller, wherein the light source provides illumination for the second digital image.

4. The combination imaging system of claim 3 , wherein the light source is mounted in the lid.

5. The combination imaging system of claim 1 , wherein the system does not include a light source for providing illumination for the second digital image, and wherein the second digital image is captured using ambient light.

6. The combination imaging system of claim 1 , wherein:
the first digital image records the intensity of light emitted by chemiluminescence from a target placed on the contact area image sensor; and
the second digital image records the intensity of light reflected from the target.

7. The combination imaging system of claim 6 , wherein the target is a blot carrying proteins tagged to emit light by chemiluminescence and also carrying protein standards that are not chemiluminescent.

8. The combination imaging system of claim 1 , further comprising a lid sensor the senses whether the lid is in the closed position or the open position.

9. The combination imaging system of claim 1 , wherein the contact area image sensor is a color sensor.

10. The combination imaging system of claim 1 , wherein the camera is a color camera.

11. The combination imaging system of claim 1 , wherein the controller is further configured to adjust the size of at least one of the first and second digital images.

12. The combination imaging system of claim 11 , wherein the controller is further configured to adjust the size of at least one of the first and second digital images so that the first and second digital images are of the same size.

13. The combination imaging system of claim 1 , wherein the controller is further configured to digitally remove distortion from at least one of the first and second digital images.

14. The combination imaging system of claim 1 , further comprising a shutter.

15. The combination imaging system of claim 14 , wherein the shutter comprises a mechanical shutter.

16. The combination imaging system of claim 14 , wherein the shutter comprises an electrical shutter.

Assignees

• Bio Rad Laboratories Inc

Inventors

• Barak Itay

Classifications

• G01N27/44726

  using specific dyes, markers or binding molecules · CPC title

• G01N21/76 Primary

  Chemiluminescence; Bioluminescence · CPC title

• G01N2201/064

  Stray light conditioning · CPC title

• G01N21/6428

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title

• G01N21/6456 Primary

  Spatial resolved fluorescence measurements; Imaging · CPC title