Slide chamber

Patent metadata

Field	Value
Publication number	US-11633741-B2
Application number	US-202016794937-A
Country	US
Kind code	B2
Filing date	Feb 19, 2020
Priority date	Mar 19, 2019
Publication date	Apr 25, 2023
Grant date	Apr 25, 2023

Abstract

The invention is directed to a slide chamber comprising a top member (10), a fluidic seal (20), a transparent closure member (30) and base member (40) wherein the top member (10) is provided with at least one examination chamber (11) and a plurality of openings (12) positioned at two sides of the cover member outside of the examination chamber (11); and the base member (40) is provided with interconnecting means (41) complementary to the openings (12) of the top member characterized in that the interconnecting means (41) of the base member (40) and the openings (12) of the top member (10) are configured to mechanically interlock with each other by lateral movement thereby pressing the top member (10) against the transparent closure (30) member in a water-tight manner.

First claim

What is claimed is:

1. A slide chamber comprising a top member ( 10 ), a fluidic seal ( 20 ), a transparent closure member ( 30 ) and base member ( 40 ) wherein the top member ( 10 ) is provided with at least one examination chamber ( 11 ) and a plurality of openings ( 12 ) positioned at two sides of the cover member outside of the examination chamber ( 11 ); and
the base member ( 40 ) is provided with interconnecting means ( 41 ) complementary to the openings ( 12 ) of the top member
characterized in that the interconnecting means ( 41 ) of the base member ( 40 ) and the openings ( 12 ) of the top member ( 10 ) are configured to mechanically interlock with each other by lateral movement the base member and the transparent closure member move laterally from an unsealed to a sealed, non-leaking position using the interconnecting means, thereby pressing the top member ( 10 ) against the transparent closure ( 30 ) member in the locked position wherein the interconnecting means ( 41 ) is configured as a bayonetted joint.

2. The slide chamber according to claim 1 , characterized in that the interconnecting means ( 41 ) and the openings ( 12 ) are configured to be mechanically disengaged from each other by lateral movement of the base member ( 40 ) against the top member ( 10 ).

3. The slide chamber according to claim 1 , characterized in that the top member ( 10 ) is provided with a recess for the fluidic seal ( 20 ).

4. The slide chamber according to claim 1 , characterized in that the top member ( 10 ) is provided with means for holding the transparent closure member ( 30 ).

5. The slide chamber according to claim 4 , characterized in that means for holding the transparent closure member ( 30 ) are configured to allow movement of the transparent closure member ( 30 ) against the top member ( 10 ).

6. The slide chamber according to claim 4 , characterized in that the means for holding the transparent closure member ( 30 ) are configured to allow movement of the transparent closure member ( 30 ) against the top member ( 10 ) before finally assembling the slide chamber.

7. The slide chamber according to claim 1 , characterized in that the top member ( 10 ) is provided with at least one grip ( 14 ).

8. The slide chamber according to claim 1 , characterized in that the interconnecting means ( 41 ) of the base member ( 40 ) and the openings ( 12 ) of the top member ( 10 ) are configured as bayonetted joint.

9. The slide chamber according to claim 1 , characterized in that the fluidic seal ( 20 ) has a Shore A hardness of 20-50.

10. The slide chamber according to claim 1 , characterized in that the base member ( 40 ) and the top member ( 10 ) is manufactured from PET, PS, PE, PP or COP.

11. The slide chamber according to claim 1 , characterized in that the transparent closure member ( 30 ) is a microscopic slide glass.

12. The slide chamber according to claim 1 , characterized in that the examination chambers are provided by walls attached to or emerging from the upper surface of the top member ( 10 ).

13. The slide chamber according to claim 1 , characterized in that the examination chambers are provided with a top cover.

14. A process for preparing at least one biological tissue in the slide chamber according to any of the claims 1 to 13 for optical examination characterized by placing the at least one biological tissue on the transparent closure member ( 30 ), combining the top member ( 10 ) and the transparent closure member ( 30 ) with an interleaved fluidic seal ( 20 );
attaching the base member ( 40 ) so that the interconnecting means ( 41 ) of the base member ( 40 ) are inserted into the openings ( 12 ) of the top member ( 10 ) and closing the slide chamber by lateral movement of the top member ( 10 ) against the transparent closure member ( 30 ).

15. The process according to claim 14 , characterized in that the at least one biological tissue is placed within at least two examination chambers.

16. The slide chamber of claim 1 , wherein the interconnecting means ( 41 ) is configured to create an increasing pressure (force) between the base member ( 40 ) and the top member ( 10 ) during the closing process, i.e. during lateral movement of the of the base member ( 40 ) against the top member ( 10 ).

17. The slide chamber of claim 16 , wherein the increasing pressure is generated by providing at least one of a tilted shape and a compressible fluidic seal ( 20 ) on at least one of the base member ( 40 ) and the openings ( 12 ), during lateral movement of the base member ( 40 ) against the top member ( 10 ).

Assignees

• Miltenyi Biotec Bv & Co Kg

Inventors

• Kabaha Eiad
• Peters Ralf-Peter
• Keseberg Jan
• Bosio Andreas

Classifications

• G01N21/03 Primary

  Cuvette constructions · CPC title

• B01L2200/025

  Align devices or objects to ensure defined positions relative to each other · CPC title

• B01L9/52 Primary

  Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips · CPC title

• B01L2300/045

  whereby the whole cover is slidable · CPC title

• B01L3/5085

  for multiple samples, e.g. microtitration plates · CPC title