Microfluidic rotor device

Patent metadata

Field	Value
Publication number	US-11628452-B2
Application number	US-201916547673-A
Country	US
Kind code	B2
Filing date	Aug 22, 2019
Priority date	Aug 24, 2018
Publication date	Apr 18, 2023
Grant date	Apr 18, 2023

Abstract

Described herein are various embodiments directed to rotor devices, systems, and kits. Embodiments of rotors disclosed herein may be used to characterize one or more analytes of a fluid. An apparatus may include a first layer being substantially transparent. A second layer may be coupled to the first layer. The second layer may be substantially absorbent to infrared radiation. The second layer and the first layer may collectively define a set of wells. The first layer may define a base for each well of the set of wells. The second layer may define an opening for each well of the set of wells. At least one of the first layer and the second layer may define a sidewall for each well of the set of wells.

First claim

The invention claimed is:

1. An apparatus, comprising:
a microfluidic rotor device defining a central hole and having a first layer being substantially transparent; and
a second layer coupled to the first layer, the second layer being substantially absorbent to infrared radiation,
the second layer and the first layer collectively defining a set of wells arranged at an equal distance about the central hole, the first layer defining a base for each well of the set of wells, the second layer defining an opening for each well of the set of wells, at least one of the first layer and the second layer defines a sidewall for each well of the set of wells, and the second layer defining a circular-shaped channel formed concentrically around the central hole,
the second layer defines a set of inlets in fluid communication with the circular-shaped channel, wherein each inlet of the set of inlets corresponding to a different well of the set of wells,
the set of inlets includes a first subset of inlets and a second subset of inlets, wherein a width of each inlet of the second subset of inlets is larger than a width of each inlet of the first subset of inlets,
bidirectional fluid occurs using the second subset of inlets but not the first subset of inlets during spinning of the microfluidic rotor device at less than about 4,000 revolutions per minute (RPMs), wherein the bidirectional fluid flow includes liquid phase flow in a first direction and a gas phase flow in a second direction;
wherein each inlet of the set of inlets establishes a fluid communication path between the opening and its corresponding well; and
wherein a width of each inlet of the second subset of inlets is between about 0.25 mm and about 3.0 mm; a length of each inlet of the second subset of inlets is between about 0.5 mm and about 6.0 mm; a depth of each inlet of the second subset of inlets is between about 0.1 mm and about 0.25 mm.

2. The apparatus of claim 1 , further comprising a third layer coupled to the second layer, the third layer defining a port configured to receive a fluid, the third layer being substantially transparent, wherein a channel establishes a fluid communication path between the port and the set of wells.

3. The apparatus of claim 2 , wherein the first layer and the third layer are each independently transparent.

4. The apparatus of claim 1 , wherein substantially transparent includes light transmission of at least one of ultraviolet light, visible light, and infrared radiation.

5. The apparatus of claim 1 , wherein the second layer is substantially absorbent to at least one of mid-infrared radiation and near-infrared radiation.

6. The apparatus of claim 1 , wherein substantially absorbent to infrared radiation includes absorbing infrared radiation in a sufficient amount within a predetermined period of time to transition a portion of the first layer from a solid phase to a molten phase.

7. The apparatus of claim 1 , wherein the second layer is substantially absorbent to 940 nm wavelength radiation.

8. The apparatus of claim 1 , wherein a diameter of the opening of each well of the set of wells is greater than a diameter of the base of each well of the set of wells.

9. The apparatus of claim 1 , wherein the sidewall of each well of the set of wells includes a first sidewall portion and a second sidewall portion, the first sidewall portion formed between the base and the second sidewall portion of that well, wherein a taper of the second sidewall portion of that well is greater than a taper of the first sidewall portion of that well.

10. The apparatus of claim 9 , wherein the first sidewall portion of that well includes a taper of up to about 2°.

11. The apparatus of claim 9 , wherein the second sidewall portion of that well includes a taper of between about 3° and about 9°.

12. The apparatus of claim 1 , wherein the opening of each well of the set of wells includes a taper of up to about 2°.

13. The apparatus of claim 9 , wherein at least one of the first sidewall portion of that well, the second sidewall portion of that well, and the opening of that well may be collectively configured as a shut off for an injection mold.

14. The apparatus of claim 1 , further comprising a lyophilized reagent disposed in at least one well of the set of wells.

15. The apparatus of claim 1 , wherein the first subset of inlets is configured for bidirectional fluid flow during spinning of the microfluidic rotor device above 3,000 revolutions per minute (RPMs).

16. The apparatus of claim 1 , wherein the second subset of inlets is configured for bidirectional fluid flow during spinning of the microfluidic rotor device between about 100 revolutions per minute (RPMs) and about 4,000 RPMs.

17. The apparatus of claim 1 , wherein the second layer defines a channel having a first end configured to receive a fluid and a second end opposite the first end, wherein the second subset of inlets are located closer to the first end than the second end.

Assignees

• Zoetis Services Llc

Inventors

• Shartle Robert Justice
• Trigub Gregory

Classifications

• B01L2300/168

  Specific optical properties, e.g. reflective coatings · CPC title

• B01L3/502715

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• B01L2400/0409

  centrifugal forces · CPC title

• B01L2200/12

  Specific details about manufacturing devices · CPC title

• B01L3/5021 Primary

  Test tubes specially adapted for centrifugation purposes · CPC title