Apparatuses and systems for preparing a meat product

Patent metadata

Field	Value
Publication number	US-11627751-B2
Application number	US-202217812315-A
Country	US
Kind code	B2
Filing date	Jul 13, 2022
Priority date	Nov 20, 2019
Publication date	Apr 18, 2023
Grant date	Apr 18, 2023

Abstract

The apparatuses described herein relate to preparation of a meat product. Apparatuses, systems comprising the apparatuses, and methods of making and use the systems and apparatuses are described herein. These are useful for controlling one or more of growth on and separation of a meat product from an enclosed substrate. The apparatuses and systems are configured to receive fluid and grow the meat product and/or separate the meat product from the substrate in a scalable manner.

First claim

We claim:

1. A method for forming cell-based meat, comprising:
providing an enclosure having a cavity and a substrate, wherein at least a portion of the substrate is non-horizontal and non-vertical relative to a ground surface; and
flowing cells suspended in a fluid media across the substrate in a manner that allows at least a portion of the cells to adhere to the substrate to form cell-based meat.

2. The method of claim 1 , further comprising recirculating unadhered cells suspended in the fluid media across the substrate.

3. The method of claim 1 , wherein the substrate has two sides and cells adhere to both sides.

4. The method of claim 3 , further comprising rotating the substrate while flowing the cells suspended in the fluid media to adhere the at least a portion of the cells to both sides of the substrate.

5. The method of claim 1 , further comprising circulating one or more gasses through the enclosure.

6. The method of claim 5 , wherein circulating the one or more gasses through the enclosure comprises circulating oxygen through the enclosure.

7. The method of claim 1 , further comprising harvesting the cell-based meat into a second enclosure where the fluid media is at least partially drained.

8. A method for growing a cell-based meat product, comprising:
providing an enclosure having a cavity and a substrate, wherein a shape of the substrate is planar and an orientation of the substrate is non-horizontal and non-vertical relative to a longitudinal axis of the enclosure;
circulating a fluid comprising cells across the substrate; and
culturing the cells into cell-based meat.

9. The method of claim 8 , further comprising recirculating unadhered cells across the substrate.

10. The method of claim 8 , wherein the substrate has two sides and circulating the fluid comprising the cells causes one or more cells to adhere to both sides of the substrate.

11. The method of claim 10 , further comprising rotating the substrate while circulating the fluid comprising the cells to seed the one or more cells on both sides of the substrate.

12. The method of claim 8 , further comprising circulating one or more gasses through the enclosure.

13. The method of claim 12 , wherein circulating the one or more gasses through the enclosure comprises circulating oxygen through the enclosure.

14. The method of claim 8 , further comprising harvesting the cell-based meat into a second enclosure where the fluid is at least partially drained.

15. A method for growing a cell-based meat product, comprising:
providing an enclosure having a cavity and a substrate, wherein the substrate is non-horizontal and non-vertical relative to a ground surface;
circulating cells suspended in nutritious media across the substrate to cause one or more cells to adhere to the substrate;
circulating the nutritious media to the one or more cells by causing the nutritious media to contact the one or more cells at a first flow rate; and
harvesting the one or more cells from the substrate by causing the nutritious media, a buffer solution, an enzymatic solution, a saline solution, a fluid including air bubbles, a fluid having higher viscosity than the nutritious media, or a combination thereof to contact the one or more cells at a second flow rate, wherein the second flow rate is higher than the first flow rate.

16. The method of claim 15 , further comprising recirculating unadhered cells across the substrate.

17. The method of claim 15 , wherein the substrate has two sides and circulating the cells causes the one or more cells to adhere to both sides of the substrate.

18. The method of claim 17 , further comprising rotating the substrate while circulating the cells to seed the one or more cells on both sides of the substrate.

19. The method of claim 15 , further comprising circulating oxygen through the enclosure.

20. The method of claim 15 , wherein harvesting further comprises directing the one or more cells into a second enclosure where fluid is at least partially drained.

21. The method of claim 15 , wherein a shape of the substrate is planar.

Assignees

• Upside Foods Inc

Inventors

• Leung Matthew
• Warner Michelle
• Vanderpol Ryan Edward
• Hsiu Thomas Pei-Ja
• Carswell Kathleen

Classifications

• C12M29/10

  Perfusion · CPC title

• C12M21/08 Primary

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title

• C12M33/00

  Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus (chemical or physical laboratory apparatus in general B01L, devices for taking cell samples A61B10/0045, withdrawing or distributing predetermined quantities of fluid B01L99/00) · CPC title

• A23L13/00 Primary

  Meat products; Meat meal; Preparation or treatment thereof · CPC title

• C12N5/0602

  Vertebrate cells · CPC title