Biomarkers for the early detection of breast cancer

Patent metadata

Field	Value
Publication number	US-11624747-B2
Application number	US-202017015702-A
Country	US
Kind code	B2
Filing date	Sep 9, 2020
Priority date	Aug 13, 2010
Publication date	Apr 11, 2023
Grant date	Apr 11, 2023

Abstract

The present invention provides reagents and methods for breast cancer detection.

First claim

We claim:

1. A polypeptide probe set comprising: a SF3A1 (SEQ ID NO: 2) isolated polypeptide (or antigenic fragment thereof) and a UBAP1 (SEQ ID NO: 47) isolated polypeptide (or antigenic fragment thereof) and one or more additional isolated polypeptides selected from the group consisting of ATP6AP1 (SEQ ID NO: 13), PDCD6IP (SEQ ID NO: 21), DBT (SEQ ID NO: 25), CSNK1E (SEQ ID NO: 9), FRS3 (SEQ ID NO: 3), HOXD1 (SEQ ID NO: 7), C15orf48 (SEQ ID NO: 35), MYOZ2 (SEQ ID NO: 33), BAT4 (SEQ ID NO: 5), BMX (SEQ ID NO: 45), RAB5A (SEQ ID NO: 23), GPR157 (SEQ ID NO: 43), ZMYM6 (SEQ ID NO: 41), SLC33A1 (SEQ ID NO: 11), TRIM32 (SEQ ID NO: 37), ALG10 (SEQ ID NO: 27), TFCP2 (SEQ ID NO: 49), SERPINH1 (SEQ ID NO: 51), SELL (SEQ ID NO: 55), ZNF510 (SEQ ID NO: 53), or antigenic fragments of any of the above; wherein each of the isolated polypeptides of the polypeptide probe set comprises a detectable tag; wherein the detectable tag is not a naturally occurring part of the isolated polypeptide; and wherein the probe set comprises no more than 100 different polypeptides.

2. The polypeptide probe set of claim 1 wherein the one or more additional isolated polypeptides comprise ATP6AP1 (SEQ ID NO: 13), or an antigenic fragment thereof.

3. The polypeptide probe set of claim 1 , wherein the probe set is present on a support.

4. The polypeptide probe set of claim 1 , wherein the probe set is present in solution.

5. The polypeptide probe set of claim 1 , wherein the probe set comprise no more than 50 different polypeptides.

6. The polypeptide probe set of claim 1 , wherein the probe set comprise no more than 25 different polypeptides.

Assignees

• Arizona Board Of Regents A Body Corporate Acting For And On Behalf Of Arizona State Univ
• Dana Farber Cancer Inst Inc
• Harvard College
• Univ Arizona

Inventors

• Labaer Joshua
• Anderson Karen Sue
• Wallstrom Garrick
• Sibani Sahar
• Ramachandran Niroshan

Classifications

• G01N33/57515 Primary

  of the breast · CPC title

• C12Q1/6886

  for cancer (immunoassay for cancer G01N33/575) · CPC title

• C40B40/10

  Libraries containing peptides or polypeptides, or derivatives thereof · CPC title

• C07K14/47

  from mammals · CPC title

• C07K17/00

  Carrier-bound or immobilised peptides (carrier-bound or immobilised enzymes C12N11/00); Preparation thereof · CPC title