Bioassisted treatment of microbiologically influenced corrosion in petroleum transporting pipelines

Patent metadata

Field	Value
Publication number	US-11624116-B2
Application number	US-202117232311-A
Country	US
Kind code	B2
Filing date	Apr 16, 2021
Priority date	Apr 17, 2020
Publication date	Apr 11, 2023
Grant date	Apr 11, 2023

Abstract

The present invention relates to a method for reducing or preventing the formation or activity of a corrosion-associated biofilm on a metal surface, wherein the method comprises contacting the metal surface with a liquid composition comprising biocidal preparation. The present invention also relates to a microbicidal composition comprising at least one alcohol, one liquid hydrocarbon, a bacteriophage immobilized on a magnetic nanocomposite, at least one phage releasing reagent and one stabilizer. The microbicide composition and method of the present invention reduces biofilms on surfaces, and consequently, reduces, mitigates, or eliminates MIC in internal surface of the oil transporting pipelines.

First claim

We claim:

1. A method for mitigating or eliminating microbially influenced corrosion (MIC) at a site of interest, the method comprising:
(i) obtaining a sample from the site of interest and identifying and culturing target bacterial strain;
(ii) identifying and obtaining bacteriophages against MIC causing microbes from the sample;
(iii) immobilizing or encapsulating the obtained bacteriophages on a nano-magnetic particle, nano-lignin or a combination thereof;
(iv) preparing a microbicide corrosion inhibitor composition by adding at least one phage releasing agent and a stabilizer to a combined solution of alcohol and hydrocarbon followed by adding immobilized or encapsulated phages;
(v) subjecting the site of interest with the microbicide composition to affect a reduction or elimination of microbially influenced corrosion (MIC)
wherein the culturing of the target bacterial strain in step (i) is performed in the presence of microbial antibiotics in a sub-optimal concentration of 1/15 th of minimum inhibitory concentration and wherein the bacteriophages are selectively released at a pH in the range of 6.5 to 4 in MIC condition, and wherein the bacteriophages are immobilized on a magnetic nanoparticle using binders having terminal aldehyde or epoxy groups and wherein the binders are selected from the group consisting of glutaraldehyde, and diglycidyl ether.

2. The method as claimed in claim 1 , wherein the immobilization of bacteriophages is attained by a process selected among ultrasonication, shaking, covalent or charge directed immobilization process.

3. The method as claimed in claim 1 , wherein the nano-magnetic particle or nano lignin is functionalized with an amine or carboxylic functionalizing agent and is having a carbon chain of length between 3 to 12; and wherein the functionalizing agent has exposed primary or secondary amine, amino acids, amine functionalized metal organic frameworks, chitosan, or N-hydroxysuccinimide, 1-ethyl-3-(3-(dimethylamino)propyl) carbodiimide hydrochloride.

4. The method as claimed in claim 1 , wherein the alcohol in the microbicide corrosion inhibitor composition is selected from the group consisting of ethanol, isopropanol, glycol, ethylene glycol, diethylene glycol, 1, 2-propylene glycol, dipropylene glycol, tripropylene glycol, glycol ether, butyl glycol, butyl diglycol, a glycol ester, butyl diglycol acetate, 2, 2, 4-trimethylpentanediol monoisobutyrate, polyethylene glycol, polypropylene glycol, benzyl alcohol, n-butyl alcohol, benzyl alcohol, 2,4-dichlorobenzyl alcohol and 2-phenoxyethanol and a combination thereof;
wherein the hydrocarbon in the microbicide corrosion inhibitor composition is selected from the group consisting of diesel, petrol, kerosene, alkenes, cyclohexane and a petroleum refining liquid fuel;
wherein the phage releasing reagent in the microbicide corrosion inhibitor composition is a protic and aprotic ionic liquid and is selected from the group consisting of 1-Benzyl-3-methylimidazolium tetrafluoroborate ([BzMIM] [BF4]), 1-Butyl-3-methyilimidazolium chloride ([BMIM] [Cl]), Triethylammonium acetate (TEAA), Choline dihydrogen phosphate ([Chol] [DHP]), Choline serinate ([Chol] [Ser]), Ethylammonium nitrate (EAN), Tetrabutylphosphonium bromide (TBPBr) and a combination thereof; and
wherein the stabilizer in the microbicide corrosion inhibitor composition is selected from the group consisting of N,N-dimethylethanolamines, such as (N,N-dimethylaminoethoxy) ethanol; dimethylethanolamine; triethanolamine; methyl diethanol amine; ethanolamine; diethanolamine; other cyclic amines including morpholine, methyl morpholine, ethylmorpholine, piperidine, alkylpiperidines, piperazine, alkylpiperazines; ethylene amines including DETA, TETA, TEPA, and the like; alkyl amines including methyl amine, dimethyl amine, alkyl methylamines, dimethyl alkylamines; methyl amino propylamine; dimethyl aminopropylamine; dimethyl aminoethylamine; methyl aminoethylamine and a combination thereof.

5. The method as claimed in claim 1 , wherein the step (v) of subjecting the site with the microbicide corrosion inhibitor composition is preceded by repetitive chain of pigging with the help of cleaning brush pig.

6. The method as claimed in claim 1 , wherein the target bacterial strain is an anaerobic bacterium causing corrosion by depositing a bacterial biofilm on a metal surface and wherein the target bacterial strain is selected from the group consisting of sulfate-reducing bacteria (SRB), iron-oxidizing/reducing bacteria (IRB), bacteria secreting organic acids (APB), exopolymers or slime (SPB), methanogen, nitrate reducing bacteria and low nutrient bacteria and a combination thereof.

7. The method as claimed in claim 1 , wherein the site of interest is a metal surface of an equipment for refining, storing, transporting of oil and natural gas.

8. The method as claimed in claim 1 , wherein the sample is a muck sample obtained from Municipal solid waste (MSW), Sewage treatment plant (STP) or industrial wastewater samples by pigging.

9. The method as claimed in claim 1 , wherein the microbicide corrosion inhibitor composition comprises bacteriophages in an amount of at least 10 9 PFU mL −1 ; at least one alcohol in amount of 50-75% (vol/vol); a hydrocarbon in an amount of 25-50% (vol/vol); at least one phage releasing agent in an amount of 1-5 ppm; and a stabilizer in an amount of 6-10 ppm is effective for reducing or eliminating microbially influenced corrosion (MIC) at the site of interest.

10. A microbicide composition for mitigating or eliminating microbiologically influenced corrosion (MIC) at a site of interest, said composition comprising:
(i) bacteriophages in an amount of at least 10 9 PFU mL −1 ;
(ii) at least one alcohol in amount of 50-75% (vol/vol);
(iii) a hydrocarbon in an amount of 25-50% (vol/vol);
(iv) at least one phage releasing agent in an amount of 1-5 ppm; and
(v) a stabilizer in an amount of 6-10 ppm.

Assignees

• Indian Oil Corp Ltd

Inventors

• Kumar Manoj
• Sahoo Prakash Chandra
• Puri Suresh Kumar
• RAMAKUMAR Sankara Sri Venkata

Classifications

• Y02E50/30

  Fuel from waste, e.g. synthetic alcohol or diesel · CPC title

• B82Y25/00

  Nanomagnetism, e.g. magnetoimpedance, anisotropic magnetoresistance, giant magnetoresistance or tunneling magnetoresistance · CPC title

• C23F15/00 Primary

  Other methods of preventing corrosion or incrustation · CPC title

• A01N63/40

  Viruses, e.g. bacteriophages · CPC title

• C09K2208/32

  Anticorrosion additives · CPC title