Bacillus isolates and uses thereof

Patent metadata

Field	Value
Publication number	US-11618879-B2
Application number	US-201816474673-A
Country	US
Kind code	B2
Filing date	Jan 3, 2018
Priority date	Jan 4, 2017
Publication date	Apr 4, 2023
Grant date	Apr 4, 2023

Abstract

The present disclosure provides isolated Bacillus strains, as well as compositions comprising one or more of the isolated strains and methods of using the isolated strains and compositions to enhance crop growth/yield.

First claim

That which is claimed:

1. An inoculant composition comprising the isolated Bacillus megaterium strain having the deposit accession number NRRL B-67357 ( B. megaterium NRRL B-67357) in an agriculturally acceptable carrier that comprises one or more monosaccharides, disaccharides, oligosaccharides, polysaccharides, maltodextrins, sugar alcohols, polyethylene glycols, polyvinylpyrrolidones, alcohol ethoxylates and/or naphthalene sulfonates.

2. The inoculant composition of claim 1 , comprising at least 1×10 4 colony-forming units of B. megaterium NRRL B-67357 per gram and/or milliliter of said inoculant composition.

3. The inoculant composition of claim 1 , wherein said agriculturally acceptable carrier is aqueous and comprises one or more disaccharides, one or more sugar alcohols and one or more polyethylene glycols.

4. The inoculant composition of claim 1 , wherein said agriculturally acceptable carrier is non-aqueous and comprises one or more disaccharides, one or more sugar alcohols and one or more polyethylene glycols.

5. The inoculant composition of claim 1 , wherein said agriculturally acceptable carrier comprises one or more polyvinylpyrrolidones.

6. The inoculant composition of claim 1 , wherein said agriculturally acceptable carrier comprises one or more alcohol ethoxylates.

7. The inoculant composition of claim 1 , wherein said agriculturally acceptable carrier comprises one or more naphthalene sulfonates.

8. A composition comprising a plant seed and a coating material that covers at least a portion of an outer surface of said plant seed, said coating material comprising the inoculant composition of claim 1 .

9. A method comprising applying the inoculant composition of claim 1 to one or more of a plant, a plant seed and plant growth medium.

10. A method comprising applying the inoculant composition of claim 2 to one or more of a plant, a plant seed and a plant growth medium.

11. A method comprising applying the inoculant composition of claim 3 to one or more of a plant, a plant seed and a plant growth medium.

12. A method comprising applying the inoculant composition of claim 4 to one or more of a plant, a plant seed and a plant growth medium.

13. A method comprising applying the inoculant composition of claim 5 to one or more of a plant, a plant seed and a plant growth medium.

14. A method comprising applying the inoculant composition of claim 6 to one or more of a plant, a plant seed and a plant growth medium.

15. A method comprising applying the inoculant composition of claim 7 to one or more of a plant, a plant seed and a plant growth medium.

16. A method comprising applying the inoculant composition of claim 8 to one or more of a plant, a plant seed and a plant growth medium.

17. A method comprising applying the inoculant composition of claim 1 to one or more of a plant, a plant seed and a plant growth medium at a rate of about 0.5 to about 100 grams and/or milliliters of said inoculant composition per plant, per kilogram of plant seed and/or per acre of plant growth medium.

18. A method comprising applying an inoculant composition that comprises at least 1×10 4 colony forming units of B. megaterium NRRL B-67357 per gram and/or milliliter of said inoculant composition to one or more of a plant, a plant seed and a plant growth medium at a rate of about 0.5 to about 100 grams and/or milliliters of said inoculant composition per plant, per kilogram of plant seed and/or per kilogram of plant growth medium.

19. A method comprising introducing a coated plant seed into a plant growth medium, wherein a portion of the outer surface of the coated plant seed is coated with an inoculant composition that comprises the isolated Bacillus megaterium strain having the deposit accession number NRRL B-67357 ( B. megaterium NRRL B-67357) in an agriculturally acceptable carrier that comprises one or more monosaccharides, disaccharides, oligosaccharides, polysaccharides, maltodextrins, sugar alcohols, polyethylene glycols, polyvinylpyrrolidones, alcohol ethoxylates and/or naphthalene sulfonates.

20. The method of claim 19 , wherein said coated plant seed comprises one or more disaccharides, one or more sugar alcohols and one or more polyethylene glycols.

Assignees

• Novozymes Bioag As

Inventors

• Blasiak Leah
• Cude William Nathan
• Kang Yaowei
• Liburn Timothy
• Sutton Kate Brandon
• Pham Jonathan

Classifications

• C12R2001/11

  Bacillus megaterium · CPC title

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• A01C1/06

  Coating or dressing seed · CPC title

• C05F11/08

  Organic fertilisers containing added bacterial cultures, mycelia or the like · CPC title

• A01P21/00

  Plant growth regulators · CPC title