Dielectrophoretic tweezer

Patent metadata

Field	Value
Publication number	US-11618042-B2
Application number	US-201815733019-A
Country	US
Kind code	B2
Filing date	Sep 25, 2018
Priority date	Sep 25, 2017
Publication date	Apr 4, 2023
Grant date	Apr 4, 2023

Abstract

The disclosure relates to a dielectrophoretic tweezer, and associated methods of fabrication and use. The tweezer comprises a first end and a second end, in which the first end has a lateral dimension of less than 10 microns; a structure, extending in a longitudinal direction between the first and second ends, comprising an electrically insulating barrier defining a first chamber and a second chamber within the structure, in which the first and second chambers are insulated from each other by the electrically insulating barrier; a first electrode in the first chamber at the first end; and a second electrode in the second chamber at the first end, in which a width of the electrically insulating barrier separating the first electrode from the second electrode is 50 nm or less.

First claim

The invention claimed is:

1. A device comprising:
a dielectrophoretic tweezer comprising:
a first end and a second end, in which the first end has a lateral dimension of less than 10 microns;
a structure, extending in a longitudinal direction between the first and second ends, comprising an electrically insulating barrier defining a first chamber and a second chamber within the structure, in which the first and second chambers are insulated from each other by the electrically insulating barrier;
a first electrode in the first chamber at the first end; and a second electrode in the second chamber at the first end,
in which a width of the electrically insulating barrier separating the first electrode from the second electrode is 50 nm or less; and

a signal generator configured to provide a time-varying voltage across the first and second electrodes of the dielectrophoretic tweezer to create a dielectrophoretic field at the first end of the dielectrophoretic tweezer.

2. The dielectrophoretic tweezer of claim 1 , in which a width of the electrically insulating barrier separating the first electrode from the second electrode is 30 nm or less.

3. The dielectrophoretic tweezer of claim 1 , in which the first electrode has a lateral dimension of 50 nm or less and in which the second electrode has a lateral dimension of 50 nm or less.

4. The dielectrophoretic tweezer of claim 1 , in which the structure has a lateral dimension of 100 nm or less at the first end.

5. The dielectrophoretic tweezer of claim 1 , in which the first and second electrodes comprise conductive carbon.

6. The dielectrophoretic tweezer of claim 1 , comprising a metallic layer on part of the electrically insulating barrier at the first end, in which the metallic layer comprises:
a first portion that extends at least partially over the first electrode;
a second portion that extends at least partially over the second electrode;
and
an electrically insulating gap between the first and second portions, the
electrically insulating gap having a width that is narrower than a width of the electrically insulating barrier.

7. The dielectrophoretic tweezer of claim 6 , in which the metallic layer is formed of a different material to the first and second electrodes and optionally comprises metal nanoparticles.

8. The dielectrophoretic tweezer of claim 6 , in which the electrically insulating gap has a width of 10 nm or less.

9. The dielectrophoretic tweezer of claim 1 , in which the first electrode and second electrode each define a semi-elliptical surface at the first end.

10. The dielectrophoretie tweezer of claim 1 , in which the first electrode and second electrode each have a non-coterminous edge region at the first end and a coterminous edge region at the first end.

11. The dielectrophoretic tweezer of claim 1 , in which the structure comprises a third chamber, in which the third chamber has openings at the first and second ends of the structure and provides a channel between the first end and the second end, and in which the third chamber is defined by the structure and is insulated and isolated from other chambers within the structure by the electrically insulating barrier.

12. The dielectrophoretic tweezer of claim 11 in which a third electrode is provided within the third chamber.

13. The dielectrophoretic tweezer of claim 11 , in which the structure comprises a fourth chamber, in which the fourth chamber has openings at the first and second ends of the structure and provides a channel between the first end and the second end, and in which the fourth chamber is defined by the electrically insulating barrier and the structure and is insulated and isolated from other chambers within the structure by the electrically insulating barrier.

14. A cell biopsy apparatus comprising:
the dielectrophoretic tweezer of claim 1 ;
a microscope having a stage for holding a sample; and
an actuator configured to actuate movement of the dielectrophoretic tweezer with respect to the stage.

15. The cell biopsy apparatus of claim 14 , in which the structure of the dielectrophoretic tweezer comprises a third chamber, in which the third chamber has openings at the first and second ends of the structure and provides a channel between the first end and the second end, and in which the third chamber is defined by the structure and is insulated and isolated from other chambers within the structure by the electrically insulating barrier;
a third electrode is provided within the third chamber; and
the microscope comprises a scanning ion-conductance microscope comprising:
a holder for holding the dielectrophoretic tweezer;
a scanning ion-conductance microscopy monitoring circuit having terminals configured to measure a current between the third electrode of the dielectrophoretic tweezer and another electrode; and
a signal generator configured to provide a time-varying voltage to the first and second electrodes of the dielectrophoretic tweezer.

16. The cell biopsy apparatus of claim 15 , comprising a controller configured to operate the scanning ion-conductance microscopy monitoring circuit during a different time period to the signal generator.

17. A method of forming a dielectrophoretic tweezer, the method comprising:
receiving a structure comprising a first end, a second end and an electrically insulating barrier, in which a first chamber and a second chamber are defined within the structure and are insulated from each other by the electrically insulating barrier, and in which a third chamber is defined by the structure and is insulated and isolated from the first chamber and the second chamber within the structure by the electrically insulating barrier, said third chamber having openings at the first end and second end of the structure and providing a channel between the first end and the second end;
pulling the structure to form a lateral dimension of less than 10 micron at the first end of the structure and a width of the electrically insulating barrier separating the first chamber from the second chamber of 50 nm or less;
depositing a conductive material to form a first electrode in the first chamber by at the first end of the structure; and
depositing a conductive material to form a second electrode in the second chamber by depositing a conductive material at the first end of the structure.

18. The method of claim 17 , inserting metal wires through respective openings at the second end until the respective metal wires contact the first and second carbon electrode at the first end.

19. A method of operating the dielectrophoretic tweezer, the dielectrophoretic tweezer comprising:
a first end and a second end, in which the first end has a lateral dimension of less than 10 microns:
a structure, extending in a longitudinal direction between the first and second ends, comprising an electrically insulating barrier defining a first chamber and a second chamber within the structure, in which the first and second chambers are insulated from each other by the electrically insulating barrier;
a first electrode in the first chamber at the first end; and a second electrode in the second chamber at the first end,
in which a width of the electrically insulating harrier separating the first electrode from the second electrode is 50 nm or less, the method comprising:
bringing the first end of the dielectrophoretic tweezer into the proximity of a molecule of interest;
applying a time-varing voltage across the first and second electrodes of the dielectrophoretic tweezer to create a dielec

Assignees

• Imperial College Sci Tech & Medicine
• Imperial College Innovations Ltd

Inventors

• Edel Joshua
• Ivanov Aleksandar
• Nadappuram Binoy Paulose
• Cadinu Paolo

Classifications

• B01L3/502761

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title

• B03C5/005 Primary

  Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength · CPC title

• G01N2001/4038

  electric methods, e.g. electromigration, electrophoresis, ionisation · CPC title

• G01Q60/44

  SICM [Scanning Ion-Conductance Microscopy] or apparatus therefor, e.g. SICM probes · CPC title