Device and method for cultivating cells

Patent metadata

Field	Value
Publication number	US-11613723-B2
Application number	US-201816621134-A
Country	US
Kind code	B2
Filing date	Jun 13, 2018
Priority date	Jun 13, 2017
Publication date	Mar 28, 2023
Grant date	Mar 28, 2023

Abstract

The present invention relates to a device for cultivating cells, in particular tissue, comprising a carrier plate unit which has a central axis of rotation, at least one access opening arranged proximally to the axis of rotation, at least one cultivation chamber arranged distally to the axis of rotation, and at least one channel connecting the access opening to the cultivation chamber, and also a method for cultivating cells in a device according to the invention and a method for producing the device according to the invention.

First claim

The invention claimed is:

1. A device for cultivating cells, comprising a carrier plate unit which has a central axis of rotation and has a plurality of access openings, wherein each access opening is arranged proximally to the axis of rotation, a plurality of cultivation chambers, wherein each cultivation chamber is arranged distally to the axis of rotation, and a plurality of channels, wherein at least one channel of the plurality of channels is associated to each access opening connecting the access opening to at least one cultivation chamber, wherein the carrier plate unit comprises at least one first carrier plate and a second carrier plate arranged above or below it, wherein the first carrier plate has the plurality of access openings, the plurality of cultivation chambers, and the plurality of channels connecting the access openings and the cultivation chambers, wherein the second carrier plate has a plurality of media openings, a plurality of media chambers, and at least one media channel connecting the media openings to the media chambers, wherein the access openings have a diameter of 0.2 to 20 mm, and wherein both, the media openings and the access openings, are accessible from outside the carrier plate unit.

2. The device as claimed in claim 1 , wherein the carrier plate unit has a central region having at least one connecting device for a rotational device.

3. The device as claimed in claim 1 , wherein the device has at least one locking device for a rotational device.

4. The device as claimed in claim 1 , wherein the at least one channel is a branched or unbranched channel.

5. The device as claimed in claim 1 , wherein the at least one channel connects the access opening to at least two cultivation chambers.

6. The device as claimed in claim 1 , wherein the channel has at least two cultivation chambers directly adjoining the channel at least over a part of its length.

7. The device as claimed in claim 1 , wherein the channel is curved at least over a part of its length.

8. The device as claimed in claim 7 , wherein the channel has a static or angle-dependent curvature.

9. The device as claimed in claim 1 , wherein the access openings are designed as loading chambers, which have at least two access openings.

10. The device as claimed in claim 1 , wherein the at least one media channel connects at least two media openings to at least one media chamber.

11. The device as claimed in claim 1 , wherein at least one separating device is arranged between the first carrier plate and the second carrier plate.

12. The device as claimed in claim 1 , wherein the cultivation chambers of the first carrier plate and the media chambers of the second carrier plate are formed overlapping and have a fluidic connection.

13. The device as claimed in claim 1 , wherein the carrier plate unit additionally comprises a reservoir for liquids.

14. The device as claimed in claim 1 , wherein the carrier plate unit has the form of a disk.

15. The device as claimed in claim 1 , wherein the carrier plate unit is designed as a micro-titration plate.

16. The device as claimed in claim 1 , wherein the carrier plate unit is constructed from glass or a polymer material.

17. The device as claimed in claim 1 , wherein the carrier plate unit is constructed from polydimethyl siloxane (PDMS) or cycloolefin copolymers (COC).

18. The device as claimed in claim 2 , wherein the at least one connecting device is a through opening or an anchoring device.

19. The device as claimed in claim 3 , wherein the locking device is peripherally arranged relative to said device.

20. The device as claimed in claim 11 , wherein the at least one separating device is a membrane.

21. A method for cultivating cells, wherein the cells are cultivated in a device as claimed in claim 1 .

22. The method as claimed in claim 21 , wherein the cultivation is performed by:
a) providing the cells and a device,
b) introducing the cells into the device through at least one access opening,
c) introducing the device into a rotational device enabling a rotation of the device,
d) setting the device into rotation,
e) receiving cells in at least one cultivation chamber, and
f) cultivating the cells in the at least one cultivation chamber.

23. The method as claimed in claim 22 , further comprising the steps of
g) introducing cell culture medium into at least one media opening,
h) setting the device into rotation, and
i) receiving cell culture in at least one media chamber to supply the cells in the cultivation chamber are carried out.

24. The method as claimed in claim 21 , wherein a continuous or pulsed flow through the media channels and media chambers with cell culture medium is enabled by the generation of a pressure gradient, external or integrated pumps, or by rotation of the device.

25. The method as claimed in claim 21 , wherein a cell complex results due to the cultivation of the cells in the at least one cultivation chamber.

26. A method for producing a cell complex, wherein a method for cultivating cells as claimed in claim 21 is carried out and a cell complex is obtained.

27. A cell culture, produced according to a method as claimed in claim 21 .

28. A method for producing a device as claimed in claim 1 , wherein in a first method step, at least one material forming the carrier plate unit is provided and this is formed into a device in a method providing shape and stability.

Assignees

• Nmi Naturwissenschaftliches Und Medizinisches Institut An Der Univ Tuebingen
• Fraunhofer Ges Forschung

Inventors

• Loskill Peter
• Schneider Oliver
• Schneider Stefan

Classifications

• C12M27/10

  Rotating vessel · CPC title

• C12M23/12 Primary

  Well or multiwell plates (C12M25/04 takes precedence) · CPC title

• C12M23/16 Primary

  Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093) · CPC title

• B01L2300/0803

  Disc shape · CPC title

• B01L3/5027

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title