Methods for Agrobacterium-mediated transformation of sugar cane
US-8952219-B2 · Feb 10, 2015 · US
US11612121B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11612121-B2 |
| Application number | US-201716093955-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 10, 2017 |
| Priority date | Apr 15, 2016 |
| Publication date | Mar 28, 2023 |
| Grant date | Mar 28, 2023 |
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Methods of producing sugar cane transplant units that includes planting a sugar cane propagation material in a planting container that has a volume of 10 to 200 cubic centimeters; growing the sugar plant to an age of at least 4 months; harvesting the stalks of the sugar cane plant when the stalks have a length of 10 to 50 centimeters; cutting the harvested stalks into stalk segments, wherein the stalk segments are cut to a length of 1 to 5 centimeters; and planting one or more of the stalk segments into a planting container that has a volume from 10 to 200 cubic centimeters.
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The invention claimed is: 1. A method of producing a sugar cane planting unit, the method comprising: a. planting a sugar cane propagation material in a first planting container to produce a sugar cane plant, wherein the sugar cane propagation material is a plant produced from tissue culture, or a sugar cane billet, and wherein the first planting container has a volume of 10 to 200 cubic centimeters; b. growing the sugar plant in the first planting container to an age of 4 to 18 months, wherein the sugar cane plant has 1 to 4 stalks and at least one stalk has a linear node density of at least 1 node per 3 centimeters of stalk; c. harvesting the stalk(s) of the sugar cane plant when the stalk(s) have a length of 10 to 50 centimeters; d. cutting the harvested stalk(s) into stalk segments, wherein the stalk segments are cut to a length of 1 to 5 centimeters; e. treating the stalk segments with water and a crop protection chemical, wherein the water temperature is 45° C. to 55° C.; and f. planting one or more of the treated stalk segments into a second planting container comprising a planting medium to form a planting unit, wherein said second planting container has a volume from 10 to 200 cubic centimeters. 2. The method of claim 1 , further comprising transplanting the planting unit into a field. 3. The method of claim 1 , wherein the crop protection chemical is a plant growth regulator. 4. The method of claim 3 , wherein the plant growth regulator is a plant hormone. 5. The method of claim 4 , wherein the plant hormone is selected from cytokinins and auxins. 6. The method of claim 4 , wherein the plant hormone is indole-3-butyric acid. 7. The method of claim 3 , further comprising treating the stalk segments with a bactericide. 8. The method of claim 7 , wherein the bactericide is selected from amicarthiazol, bismerthiazol, bronopol, cellocidin, chloramphenicol, copper ammonium carbonate, copper hydroxide, copper octanoate, copper oxychloride, copper oxides, copper sulfate, copper salts of fatty acids, cresol, dichlorophen, dipyrithione, dodicin, ethylicin, fenaminosulf, formaldehyde, hexachlorophene, hydrated lime, hydrargaphen, 8-hydroxyquinoline sulfate, kasugamycin, nitrapyrin, octhilinone, oxolinic acid, oxytetracycline, phenazine oxide, probenazole, saijunmao, saisentong, silver nitrate, streptomycin, tecloftalam, thiodiazole-copper, thiomersal, xinjunan, and zinc thiazole. 9. The method of claim 8 , wherein the bactericide is silver nitrate. 10. The method of claim 1 , wherein the water temperature is 48° C. to 53° C. 11. The method of claim 1 , wherein treating the stalk segments with water comprises immersing the stalk segments for a soak time of 1 to 10 minutes. 12. The method of claim 11 , wherein the water temperature is 48° C. to 53° C. 13. The method of claim 12 , wherein the soak time is 2 to 3 minutes. 14. The method of claim 3 , wherein the treating the stalk segments with the plant growth regulator comprises immersing the stalk segment in a plant growth regulator solution having a plant growth regulator concentration of 0.01 to 100 g/L. 15. The method of claim 14 , wherein the plant growth regulator is indole-3-butyric acid and the indole-3-butyric acid concentration is 0.5 to 10 g/L. 16. The method of claim 9 , wherein the treating the stalk segments with silver nitrate comprises immersing the stalk segment in a silver nitrate solution having a silver nitrate concentration of 0.01 to 10 g/L. 17. The method of claim 1 , wherein the crop protection chemical is a fungicide or insecticide. 18. The method of claim 1 , wherein the planting the one or more stalk segments comprises planting a single stalk segment into the second planting container. 19. The method of claim 1 , wherein the sugar cane propagation material is a sugar cane billet; and wherein the sugar cane billet consists of a single node. 20. A method of producing a sugar cane planting unit, the method comprising: a. planting one or more stalk segments into a second planting container comprising a planting medium to form a planting unit, wherein said second planting container has a volume from 10 to 200 cubic centimeters, and wherein the one or more stalk segments was produced by the steps comprising: i. planting a sugar cane propagation material in a first planting container to produce a sugar cane plant, wherein the sugar cane propagation material is a plant produced from tissue culture, or a sugar cane billet, and wherein the first planting container has a volume of 10 to 200 cubic centimeters; ii. growing the sugar plant in the first planting container to an age of 4 to 18 months, wherein the sugar cane plant has 1 to 4 stalks and at least one stalk has a linear node density of at least 1 node per 3 centimeters of stalk; iii. harvesting the stalk(s) of the sugar cane plant when the stalk(s) have a length of 10 to 50 centimeters; iv. cutting the harvested stalk(s) into stalk segments, wherein the stalk segments are cut to a length of 1 to 5 centimeters; and v. treating the stalk segments with water and a crop protection chemical.
Plant reproduction by tissue culture techniques {; Tissue culture techniques therefor} · CPC title
by means of cuttings · CPC title
Methods or apparatus for planting not provided for in other groups of this subclass · CPC title
Heavy metals; Compounds thereof · CPC title
Sugar cane · CPC title
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