Method for detecting a radical compound derived from a lipid and a compound derived from the radical compound
US-2018328951-A1 · Nov 15, 2018 · US
US11609238B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11609238-B2 |
| Application number | US-201815965096-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 27, 2018 |
| Priority date | Apr 28, 2017 |
| Publication date | Mar 21, 2023 |
| Grant date | Mar 21, 2023 |
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[Problem] To provide a method of scavenging an unstable radical derived from a lipid and analyzing structures of those radicals.[Means for solution] A fluorescent nitroxide (NBD-Pen) is made to act to scavenge a lipid radical or a fragment radical thereof, and fluorescent detection liquid chromatography (LC/FL) and mass spectrometry (MS) are combined to identify lipid-derived radicals contained in a lipid extract.
Opening claim text (preview).
The invention claimed is: 1. A compound, which is the following compound (C′): wherein R is selected from a group consisting of CH 3 , C 2 H 5 , C 3 H 7 , C 4 H 9 , C 6 H 13 , C 7 H 13 , C 7 H 13 O, C 8 H 15 O, C 9 H 15 , C 9 H 15 O 2 , C 9 H 13 O 3 , C 10 H 17 O, C 11 H 19 O 2 , and C 12 H 21 O 2 ; or R is selected from a group consisting of AA-O 2 , CH 3 , C 2 H 5 , C 3 H 7 , C 4 H 9 , C 6 H 13 , C 6 H 9 O 3 , C 7 H 13 , C 7 H 13 O, C 8 H 13 O 2 , C 8 H 15 O, C 9 H 17 , C 9 H 15 O 2 , C 9 H 13 O 3 , C 10 H 17 O, C 11 H 19 , C 11 H 17 O, C 11 H 19 O, C 11 H 19 O 2 , C 12 H 19 O, C 12 H 19 O 2 , C 13 H 19 O 2 , C 14 H 21 O, C 14 H 23 O, and C 15 H 23 O, and wherein AA represents arachidonic acid; or R is selected from a group consisting of ALA-O 2 , CH 3 , C 5 H 9 O, C 6 H 9 O, C 6 H 11 O, C 7 H 11 O, C 8 H 13 , C 8 H 11 O, C 8 H 11 O 3 , C 8 H 13 O, C 9 H 13 O, C 9 H 13 O 2 , C 10 H 15 O, C 11 H 17 , C 11 H 15 O, C 11 H 17 O, C 11 H 15 O 3 , C 15 H 21 O 2 , and C 15 H 21 O 3 and, wherein ALA represents α-linolenic acid; or R is selected from a group consisting of EPA-O 2 , CH 3 , C 5 H 9 O, C 6 H 9 O, C 6 H 11 O, C 7 H 11 O, C 8 H 13 , C 8 H 11 O, C 8 H 11 O 3 , C 8 H 13 O, C 9 H 13 O, C 9 H 13 O 2 , C 10 H 15 O, C 11 H 17 , C 11 H 15 O, C 11 H 17 O, C 11 H 15 O 3 , C 12 H 17 O, C 12 H 17 O 2 , C 14 H 21 O, C 14 H 19 O, C 15 H 21 O, C 15 H 21 O 2 , C 15 H 21 O 3 , C 17 H 23 O, and C 18 H 25 O and, wherein EPA represents eicosapentaenoic acid; or R is selected from a group consisting of DHA-O 2 , CH 3 , C 5 H 9 O, C 6 H 9 O, C 6 H 11 O, C 7 H 11 O, C 8 H 13 , C 8 H 11 O, C 8 H 11 O 3 , C 8 H 13 O, C 9 H 13 O, C 9 H 13 O 2 , C 10 H 15 O, C 11 H 17 , C 11 H 15 O, C 11 H 17 O, C 11 H 15 O 3 , C 12 H 17 O, C 12 H 17 O 2 , C 14 H 21 O, C 14 H 19 O, C 15 H 21 O, C 15 H 21 O 2 , C 15 H 21 O 3 , C 17 H 23 O, C 18 H 25 O, and C 21 H 31 O 4 and, wherein DHA represents docosahexaenoic acid. 2. The compound of claim 1 , wherein R is selected from a group consisting of C 7 H 13 , C 7 H 13 O, C 8 H 15 O, C 9 H 15 , C 9 H 15 O 2 , C 9 H 13 O 3 , C 10 H 17 O, C 11 H 19 O 2 , and C 12 H 21 O 2 ; or R is selected from a group consisting of AA-O 2 , C 6 H 9 O 3 , C 7 H 13 , C 7 H 13 O, C 8 H 13 O 2 , C 8 H 15 O, C 9 H 17 , C 9 H 15 O 2 , C 9 H 13 O 3 , C 10 H 17 O, C 11 H 19 , C 11 H 17 O, C 11 H 19 O, C 11 H 19 O 2 , C 12 H 19 O, C 12 H 19 O 2 , C 13 H 19 O 2 , C 14 H 21 O, C 14 H 23 O, and C 15 H 23 O, and wherein AA represents arachidonic acid; or R is selected from a group consisting of ALA-O 2 , C 5 H 9 O, C 6 H 9 O, C 6 H 11 O, C 7 H 11 O, C 8 H 13 , C 8 H 11 O, C 8 H 11 O 3 , C 8 H 13 O, C 9 H 13 O, C 9 H 13 O 2 , C 10 H 15 O, C 11 H 17 , C 11 H 15 O, C 11 H 17 O, C 11 H 15 O 3 , C 15 H 21 O 2 , and C 15 H 21 O 3 and, wherein ALA represents α-linolenic acid; or R is selected from a group consisting of EPA-O 2 , C 5 H 9 O, C 6 H 9 O, C 6 H 11 O, C 7 H 11 O, C 8 H 13 , C 5 H 11 O, C 8 H 11 O 3 , C 8 H 13 O, C 9 H 13 O, C 9 H 13 O 2 , C 10 H 15 O, C 11 H 17 , C 11 H 15 O, C 11 H 17 O, C 11 H 15 O 3 , C 12 H 17 O, C 12 H 17 O 2 , C 14 H 21 O, C 14 H 19 O, C 15 H 21 O, C 15 H 21 O 2 , C 15 H 21 O 3 , C 17 H 23 O, and C 18 H 25 O and, wherein EPA represents eicosapentaenoic acid; or R is selected from a group consisting of DHA-O 2 , C 5 H 9 O, C 6 H 9 O, C 6 H 11 O, C 7 H 11 O, C 8 H 13 , C 5 H 11 O, C 8 H 11 O 3 , C 8 H 13 O, C 9 H 13 O, C 9 H 13 O 2 , C 10 H 15 O, C 11 H 17 , C 11 H 15 O, C 11 H 17 O, C 11 H 15 O 3 , C 12 H 17 O, C 12 H 17 O 2 , C 14 H 21 O, C 14 H 19 O, C 15 H 21 O, C 15 H 21 O 2 , C 15 H 21 O 3 , C 17 H 23 O, C 18 H 25 O, and C 21 H 31 O 4 and, wherein DHA represents docosahexaenoic acid.
Sources of fatty acids, e.g. natural glycerides, characterised by the nature, the quantities or the distribution of said acids · CPC title
involving lipids, e.g. cholesterol {, lipoproteins, or their receptors (steroid hormones G01N33/743)} · CPC title
condensed with carbocyclic rings or ring systems · CPC title
linked by a chain containing hetero atoms as chain links · CPC title
with fluorescent label · CPC title
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