Fluid supply interface having a safety valve for a cell culture system, use of such a fluid supply interface for managing cell culture containers, and cell culture management system

Patent metadata

Field	Value
Publication number	US-11608487-B2
Application number	US-201716310656-A
Country	US
Kind code	B2
Filing date	Jun 14, 2017
Priority date	Jun 17, 2016
Publication date	Mar 21, 2023
Grant date	Mar 21, 2023

Abstract

A fluid supply interface includes at least one supply coupling configuration, at least one discharge coupling configuration, at least one user coupling configuration, and a fluid conduit that connects the supply, discharge, and user coupling configurations to one another. A supply valve is capable of having fluid flow through it or is blocked for flow through it. A discharge valve is capable of having fluid flow through it or is blocked for flow through it. The supply valve is preloaded into a blocking position that prevents flow, and opens by means of a sufficiently large pressure difference between the two sides of the supply valve, against the preload force, for flow through in a direction from the supply coupling configuration toward the fluid conduit. The discharge valve likewise being preloaded into a closed position that prevents flow through it.

First claim

The invention claimed is:

1. A fluid supply interface for a cell culture system, the fluid supply interface comprising:
at least one supply coupling configuration for receiving a respective supply fluid line;
at least one discharge coupling configuration for receiving a respective discharge fluid line, different from the supply fluid line;
at least one user coupling configuration for receiving a respective user fluid line, different from the supply fluid line and the discharge fluid line;
a fluid conduit that connects the at least one supply coupling configuration, the at least one discharge coupling configuration, and the at least one user coupling configuration to one another;
at least one supply valve by means of which, depending on an operation position of the at least one supply valve, a supply coupling configuration of the at least one supply coupling configuration is capable of having fluid flow therethrough or is blocked for flow therethrough;
at least one discharge valve, different from the at least one supply valve, by means of which, depending on an operation position of the at least one discharge valve, a discharge coupling configuration of the at least one discharge coupling configuration is capable of having fluid flow therethrough or is blocked for flow therethrough; and
a retaining configuration embodied in the fluid conduit;
wherein the at least one supply valve being preloaded into a blocking position that prevents flow, and opening by means of a sufficiently large pressure difference between two sides of the at least one supply valve, against the preload force, for flow through in a direction from the at least one supply coupling configuration toward the fluid conduit, the at least one discharge valve likewise being preloaded into a closed position that prevents flow therethrough,
wherein the at least one discharge valve, constituting a safety valve, is embodied to open upon exceedance of a predetermined pressure difference between the two sides of the safety valve, against its preload force, for flow through in a direction away from the fluid conduit and toward the at least one discharge coupling configuration,
wherein, each of the at least one supply valve and the at least one discharge valve includes a valve body,
wherein, when the valve body of the at least one supply valve and/or the at least one discharge valve is lifted off a valve seat, the retaining configuration retains the valve body of the at least one supply valve or the at least one discharge valve in an influence region of the preload force,
wherein the at least one discharge valve and the at least one supply valve comprise the same valve bodies;
wherein the flow cross section of the fluid conduit in a portion adjacent to the retaining configuration is smaller than a cross-sectional dimension of the valve body of the at least one supply valve and the valve body the at least one discharge valve orthogonally to the path along which the fluid conduit proceeds in the adjacent portion, so that penetration of the valve body of the at least one supply valve and the valve body the at least one discharge valve into the fluid conduit is prevented.

2. The fluid supply interface according to claim 1 , wherein the safety valve comprises a mechanical preload device.

3. The fluid supply interface according to claim 1 , wherein the valve body of the safety valve having or made of ferromagnetic and/or permanently magnetized material and is switchable between a closed position and a flowthrough position by modification of an external magnetic field, and/or the valve body of the at least one supply valve having or made of ferromagnetic and/or permanently magnetized material and is switchable between a blocking position and a passthrough position by modification of an external magnetic field.

4. The fluid supply interface according to claim 1 , wherein the fluid conduit encloses an angle with the lifting direction of the valve body of the at least one supply valve and/or of at least one discharge valve.

5. The fluid supply interface according to claim 1 , wherein the at least one supply coupling configuration comprises a plurality of supply coupling configurations, which
are connected to one another by the fluid conduit, and
each comprise a supply valve that
is preloaded into a blocking position in which flow through the respective supply coupling configuration is prevented, and
as a result of a sufficiently large pressure difference between the two sides of the supply valve, opens against the preload force for flow through in a direction from the respective supply coupling configuration toward the fluid conduit,
wherein the fluid supply interface includes a switching device having at least one magnet with which, at a supply switching location, an eternal magnetic field generated by the at least one magnet is modifiable so that a supply valve of the one of the plurality of supply configurations provided at the supply switching location can be switched between its operation positions, a number of supply switching locations being smaller than the number of supply valves.

6. The fluid supply interface according to claim 5 , wherein the at least one discharge valve is also switchable between its operating positions by the switching device.

7. The fluid supply interface according to claim 6 , wherein the at least one discharge coupling configuration comprises a plurality of discharge coupling configurations that are connected to one another by the fluid conduit and each comprise a discharge valve of the one of the plurality of discharge coupling configuration are provided, such that an external magnetic field generated by the at least one magnet is modifiable by the switching device at a discharge switching location so that a discharge valve provided at the discharge switching locations being the same as or smaller than the number of discharge valves.

8. The fluid supply interface according to claim 5 , wherein the external magnetic field generated by the at least one magnet is also modifiable by the switching device at a user switching location that is located in the region of the user coupling configuration and at which a user fluid line having a user valve can temporarily be arranged, so that the user valve provided at the user switching location can be switched between a locked position and a flowthrough position.

9. The fluid supply interface according to claim 1 , wherein the fluid supply interface is coupled to a backwash apparatus by means of which a given fluid is drivable to flow in opposite directions in the fluid conduit.

10. The fluid supply interface according to claim 9 , wherein the backwash apparatus encompasses a reversible-direction pump.

11. The fluid supply interface according to claim 10 , wherein a backwash fluid line that connects the reversible-direction pump to the fluid conduit is provided upstream, in a delivery flow direction from the at least one supply coupling configuration to the at least one user coupling configuration, from the at least one supply valve.

12. A cell culture management system having a fluid reservoir, having a discharge sink, and having a fluid supply interface according to claim 1 , wherein the fluid reservoir is coupled via a supply fluid line to the at least one supply coupling configuration, the discharge sink is coupled via a discharge fluid line to the at least one discharge coupling configuration, and a cell culture container is detachably coupled onto the at least one user coupling configuration.

13. The fluid supply interface according to claim 1 , wherein the safety valve comprises a spring device.

14. The fluid

Assignees

• Hamilton Bonaduz Ag

Inventors

• Kühne Oliver

Classifications

• C12M33/07

  Dosage or metering devices therefore · CPC title

• C12M23/40 Primary

  Manifolds; Distribution pieces (fluid transfer means B01L3/563) · CPC title

• C12M41/40 Primary

  of pressure · CPC title

• C12M41/00

  Means for regulation, monitoring, measurement or control, e.g. flow regulation (controlling or regulating chemical, physical or physicochemical processes B01J19/0006; heating or cooling apparatus for laboratory use B01L7/00; electro optical investigation of individual particles, flow cytometers G01N15/14; automatic analysis G01N35/00; controlling or regulating in general G06N) · CPC title

• C12M29/00

  Means for introduction, extraction or recirculation of materials, e.g. pumps (pumps per se F04B) · CPC title