Methods of sperm cell sensing utilizing an avalanche photodiode and cytometer apparatus

Patent metadata

Field	Value
Publication number	US-11600736-B2
Application number	US-202217660556-A
Country	US
Kind code	B2
Filing date	Apr 25, 2022
Priority date	Sep 15, 2017
Publication date	Mar 7, 2023
Grant date	Mar 7, 2023

Abstract

A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.

First claim

What is claimed is:

1. A system for sorting particles in a flow cytometry apparatus, the system comprising:
an electromagnetic radiation source configured to emit an interrogation beam of electromagnetic radiation at a particle in an interrogation location of the flow cytometry apparatus;
avalanche photodiode circuitry comprising at least one avalanche photodiode configured to detect an emission of electromagnetic radiation by the particle at the interrogation location, the at least one avalanche photodiode further configured to output a signal based on the detected emission; and
a set of charge plates to deflect differentially charged droplets, wherein the differentially charged droplets are charged based on the output signal of the avalanche photodiode.

2. The system of claim 1 wherein the flow cytometry apparatus comprises a flow chamber that directs a fluid containing the particle to the interrogation location.

3. The system of claim 2 wherein the flow chamber comprises the at least one avalanche photodiode.

4. The system of claim 2 wherein the flow chamber comprises a multiplexer.

5. The system of claim 1 , wherein the particle is a sperm cell.

6. The system of claim 1 , wherein the particle comprises a particle stained by a dye.

7. The system of claim 6 , wherein the dye is a DNA-interlacing dye.

8. The system of claim 1 , wherein the emission of electromagnetic radiation by the particle at the interrogation location is a fluorescence caused by an irradiation of the particle by the interrogation beam of electromagnetic radiation emitted by the electromagnetic radiation source.

9. The system of claim 1 , wherein an input to the avalanche photodiode is controlled by a feedback loop.

10. The system of claim 1 , wherein the source of electromagnetic radiation comprises a laser.

11. A method for assessing an amount of DNA within a nucleus of a sperm cell comprising:
staining the DNA within the nucleus of the sperm cell;
irradiating the stained DNA within the nucleus of the sperm cell; and
detecting, with an avalanche photodiode, fluorescent light emitted from the irradiated and stained DNA within the nucleus of the sperm cell;
charging differentially charged droplets with an output signal of the avalanche photodiode; and
deflecting differentially charged droplets with a set of charge plates.

12. The method of claim 11 further comprising differentiating X chromosome bearing sperm cells and Y chromosome bearing sperm cells by:
determining a sex of the sperm cell using the detected amount of DNA within the nucleus of the sperm cell; and
differentiating between a plurality of sperm cells based upon the sex determination.

13. The method of claim 12 wherein characteristics of the X chromosome bearing sperm cells and Y chromosome bearing sperm cells comprise differentiable amounts of DNA within the nuclei of the sperm cell.

14. The method of claim 13 further comprising photo-damaging a given sperm cell based upon an amount of DNA within the nucleus of the given sperm cell.

15. The method of claim 14 further comprising:
entraining a plurality of sperm cells with a plurality of the differentially charged droplets; and
differentially collecting each of the charged droplets based upon the sex determination of the sperm cells entrained in the charged droplets.

16. A method for causing the deactivation of particles in a flow cytometry apparatus, the method comprising:
emitting an interrogation beam of electromagnetic radiation by a first electromagnetic radiation source, the interrogation beam of electromagnetic radiation directed at a particle in an interrogation location of the flow cytometry apparatus;
detecting, by an avalanche photodiode, an emission of electromagnetic radiation by the particle at the interrogation location;
outputting, by the avalanche photodiode, an output signal based on the detected emission of electromagnetic radiation by the particle at the interrogation location; and
deflecting differentially charged droplets with a set of charge plates, wherein the differentially charged droplets are charged based on the output signal of the avalanche photodiode.

17. The method of claim 16 further comprising relating a quantum efficiency of the avalanche photodiode to the cytometer through a chosen dye and optical excitation wavelength.

18. The method of claim 17 further comprising incorporating the avalanche photodiode into the cytometer.

19. The method of claim 18 further comprising combining an optical gain factor of the avalanche photodiode with the quantum efficiency to produce the output signal.

20. The method of claim 16 further comprising entraining a plurality of sperm cells with a plurality of the differentially charged droplets.

Assignees

• Abs Global Inc

Inventors

• Savage Frederick
• Szejna Glenn J
• Xia Zheng

Classifications

• H10F77/60

  Arrangements for cooling, heating, ventilating or compensating for temperature fluctuations · CPC title

• H10F30/2255 Primary

  in which the active layers form heterostructures, e.g. SAM structures · CPC title

• H04B10/69

  Electrical arrangements in the receiver · CPC title

• G01N21/6486 Primary

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• C12N2529/10

  Stimulation by light · CPC title