Method of analysing an aqueous fluid using 2D-IR spectroscopy

Patent metadata

Field	Value
Publication number	US-11598718-B2
Application number	US-201916972345-A
Country	US
Kind code	B2
Filing date	Jun 7, 2019
Priority date	Jun 7, 2018
Publication date	Mar 7, 2023
Grant date	Mar 7, 2023

Abstract

A method of analysing an aqueous fluid comprising obtaining a 2D-IR spectrum of a sample of the aqueous fluid using a 2D-IR spectrometer configured to apply a sequence of IR pulses to the sample, wherein the sequence comprises a pump process followed by a probe pulse, where the pump process is a single pump pulse or a sequence of a first pump pulse and a second pump pulse, and a waiting time T w between applying the single pump pulse or the second pump pulse and applying the probe pulse is from 150 to 350 fs.

First claim

The invention claimed is:

1. A method of analyzing an aqueous fluid, said method comprising:
obtaining a 2D-IR spectrum of a sample of the aqueous fluid using a 2D-IR spectrometer configured to apply a sequence of IR pulses to the sample, and
determining whether or not one or more proteins are present in the aqueous fluid by observing the amide I band of the one or more proteins,
wherein the sequence comprises a pump process followed by a probe pulse, where the pump process is a single pump pulse or a sequence of a first pump pulse and a second pump pulse, and a waiting time T w between applying the single pump pulse or the second pump pulse and applying the probe pulse is from 150 to 350 fs.

2. The method according to claim 1 , wherein the pump process is a sequence of a first pump pulse and a second pump pulse and T w is time between applying the second pump pulse and applying the probe pulse.

3. The method according to claim 2 , wherein T w is from 200 to 300 fs.

4. The method according to claim 1 , wherein T w is from 200 to 300 fs.

5. The method according to claim 1 , further comprising identifying at least one peak by comparing the 2D-IR spectrum with one or more reference 2D-IR spectra.

6. The method according to claim 1 , further comprising determining whether or not albumin is present in the aqueous fluid by determining if the spectrum has a peak at a frequency of 1656 cm −1 and/or determining if γ-globulins are present in the aqueous fluid by determining if the spectrum has a peak at a frequency of 1639 cm −1 .

7. The method according to claim 1 , further comprising identifying at least one peak and quantifying the at least one peak using a calibration based on one or more reference 2D-IR spectra.

8. The method according to claim 1 , wherein the 2D-IR spectrum comprises peaks due to albumin and globulins and the method comprises determining a ratio of albumin to globulins present in the sample.

9. The method according to claim 1 , wherein the 2D-IR spectrum comprises a peak due to albumin and a peak due to γ-globulins and the method comprises determining a ratio of albumin to γ-globulins by measuring a peak height of the albumin peak and a peak height of the γ-globulins peak and dividing the peak height of the albumin peak by the peak height of the γ-globulins peak multiplied by 1.8.

10. The method of diagnosing and/or prognosing an abnormality in a subject comprising analyzing a sample of an aqueous biofluid from the subject using the method of claim 9 .

11. A method according to claim 10 , wherein the method comprises determining from the 2D-IR spectrum whether treatment of the subject or further testing of the subject is required.

12. A method according to claim 10 , wherein the abnormality is poor general health or the presence of an inflammatory response, and the method involves determining the ratio of albumins to γ-globulins in the aqueous biofluid.

13. A method according to claim 10 , wherein the 2D-IR spectrum is compared to a plurality of pre-correlated spectra stored in a database in order to derive a correlation with a favorable or unfavorable diagnosis or wherein the spectrum is correlated with a favorable or unfavorable diagnosis based on a predictive model developed by “training” a database of pre-correlated analyses.

14. The method according to claim 1 , wherein the aqueous fluid is an aqueous biofluid.

15. The method according to claim 14 , wherein the method comprises obtaining a sample of the aqueous biofluid from the subject.

16. A method according to claim 15 , wherein the method comprises determining from the 2D-IR spectrum whether treatment of the subject or further testing of the subject is required.

17. A method according to claim 15 , wherein the abnormality is poor general health or the presence of an inflammatory response, and the method involves determining the ratio of albumins to γ-globulins in the aqueous biofluid.

18. A method according to claim 17 , wherein the 2D-IR spectrum is compared to a plurality of pre-correlated spectra stored in a database in order to derive a correlation with a favorable or unfavorable diagnosis or wherein the spectrum is correlated with a favorable or unfavorable diagnosis based on a predictive model developed by “training” a database of pre-correlated analyses.

19. A method of analyzing an aqueous fluid, said method comprising:
obtaining a 2D-IR spectrum of a sample of the aqueous fluid using a 2D-IR spectrometer configured to apply a sequence of IR pulses to the sample, the 2D-IR spectrum including peaks due to albumin and globulins, and
determining a ratio of albumin to globulins present in the sample,
wherein the sequence comprises a pump process followed by a probe pulse, where the pump process is a single pump pulse or a sequence of a first pump pulse and a second pump pulse, and a waiting time T w between applying the single pump pulse or the second pump pulse and applying the probe pulse is from 150 to 350 fs.

20. A method of analyzing an aqueous fluid, said method comprising:
obtaining a 2D-IR spectrum of a sample of the aqueous fluid using a 2D-IR spectrometer configured to apply a sequence of IR pulses to the sample, the 2D-IR spectrum including a peak due to albumin and a peak due to γ-globulins, and
determining a ratio of albumin to γ-globulins by measuring a peak height of the albumin peak and a peak height of the γ-globulins peak and dividing the peak height of the albumin peak by the peak height of the γ-globulins peak multiplied by 1.8,
wherein the sequence comprises a pump process followed by a probe pulse, where the pump process is a single pump pulse or a sequence of a first pump pulse and a second pump pulse, and a waiting time T w between applying the single pump pulse or the second pump pulse and applying the probe pulse is from 150 to 350 fs.

Assignees

• Univ York

Inventors

• Baker Matthew
• Hunt Neil T
• Rutherford Samantha
• Hithell Gordon

Classifications

• G01N21/636

  using an arrangement of pump beam and probe beam; using the measurement of optical non-linear properties; (non-linear optics per se G02F1/35) · CPC title

• G01N21/3577 Primary

  for analysing liquids, e.g. polluted water · CPC title

• G01N33/49

  Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} · CPC title

• G01N33/487

  of liquid biological material · CPC title

• G01N2021/3595

  using FTIR · CPC title