Bioproduction of phenethyl alcohol, aldehyde, acid, amine, and related compounds

Patent metadata

Field	Value
Publication number	US-11597954-B2
Application number	US-201816615689-A
Country	US
Kind code	B2
Filing date	May 23, 2018
Priority date	May 23, 2017
Publication date	Mar 7, 2023
Grant date	Mar 7, 2023

Abstract

This invention relates to the bioproduction of substituted or unsubstituted phenylacetaldehyde, 2-phenylethanol, phenylacetic acid or phenylethylamine by subjecting a starting material comprising glucose, L-phenylalanine, substituted L-phenylalanine, styrene or substituted styrene to a plurality of enzyme catalyzed chemical transformations in a one-pot reaction system, using recombinant microbial cells overexpressing the enzymes. To produce phenylacetaldehyde from styrene, the cells are modified to overexpress styrene monooxygenase (SMO) and styrene oxide isomerase (SOI). To produce phenylacetic acid from styrene, SMO, SOI and aldehyde dehydrogenase are overexpressed. Alternatively, to produce 2-phenylethanol, SMO, SOI and aldehyde reductase or alcohol dehydrogenase are overexpressed, while to produce phenylethylamine, SMO, SOI and transaminase are overexpressed.

First claim

The invention claimed is:

1. A method for bioproduction of substituted or unsubstituted phenylacetaldehyde, 2-phenylethanol, phenylacetic acid or phenylethylamine by one or more recombinant bacterial or fungal cells genetically engineered to overexpress, relative to a wild type cell, at least one enzyme, which method comprises subjecting a starting material to a plurality of enzyme-catalyzed chemical transformations in an one-pot reaction system, wherein the starting material is selected from the group consisting of glucose, L-phenylalanine, substituted L-phenylalanine, styrene and substituted styrene, wherein the genetically engineered cells:
i) overexpress styrene monooxygenase and styrene oxide isomerase for generating substituted or unsubstituted phenylacetaldehyde from the styrene or the substituted styrene, wherein the styrene monooxygenase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NOs: 1 and 2; and the styrene oxide isomerase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO: 3; or
ii) overexpress styrene monooxygenase, styrene oxide isomerase and an aldehyde dehydrogenase for generating substituted or unsubstituted phenylacetic acid from the styrene or the substituted styrene, wherein the styrene monooxygenase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NOs: 1 and 2; the styrene oxide isomerase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO: 3; and the aldehyde dehydrogenase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO: 4; or
iii) overexpress styrene monooxygenase, styrene oxide isomerase, an aldehyde reductase and/or an alcohol dehydrogenase for generating substituted or unsubstituted 2-phenylethanol from the styrene or the substituted styrene, wherein the styrene monooxygenase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NOs: 1 and 2; the styrene oxide isomerase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO: 3; and the alcohol dehydrogenase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO: 5; or
iv) overexpress styrene monooxygenase, styrene oxide isomerase and a transaminase for generating substituted or unsubstituted phenylethylamine from the styrene or the substituted styrene, wherein the styrene monooxygenase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NOs: 1 and 2; the styrene oxide isomerase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO: 3; and the transaminase is ω-transaminase comprising an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO: 6.

2. The method of claim 1 , wherein the styrene monooxygenase is encoded by a nucleic acid sequence having at least 85% identity to the nucleic acid sequence set forth in SEQ ID NO: 7 and 8; the styrene oxide isomerase is encoded by a nucleic acid sequence having at least 85% identity to the nucleic acid sequence set forth in SEQ ID NO: 9; the aldehyde dehydrogenase is encoded by a nucleic acid sequence having at least 85% identity to the nucleic acid sequence set forth in SEQ ID NO: 10; the alcohol dehydrogenase is encoded by a nucleic acid sequence having at least 85% identity to the nucleic acid sequence set forth in SEQ ID NO: 11 and the transaminase is w-transaminase encoded by a nucleic acid sequence having at least 85% identity to the nucleic acid sequence set forth in SEQ NO: 12.

3. The method of claim 1 , wherein the genetically engineered cells produce styrene or substituted styrene from L-phenylalanine or substituted L-phenylalanine by a deamination reaction catalyzed by overexpression of an ammonia lyase and a decarboxylation reaction catalyzed by overexpression of a decarboxylase.

4. The method of claim 3 , wherein the ammonia lyase comprises the amino acid sequence set forth in SEQ ID NO: 13 and the decarboxylase comprises the amino acid sequence set forth in SEQ ID NO: 14.

5. The method of claim 1 , wherein the genetically engineered cells produce L-phenylalanine from glucose by a reaction catalyzed by overexpression of at least one enzyme selected from a group comprising DAHP synthase (AroG), shikimate kinase (AroK), shikimate dehydrogenase (YdiB), chorismate mutase/prephenate dehydratase (PheA) and tyrosine aminotransferase (TyrB), wherein the genetically engineered cells are engineered to produce L-phenylalanine from glucose.

6. The method of claim 5 , wherein AroG comprises the amino acid sequence set forth in SEQ ID NO: 17; AroK comprises the amino acid sequence set forth in SEQ ID NO: 18; YdiB comprises the amino acid sequence set forth in SEQ ID NO: 19; PheA comprises the amino acid sequence set forth in SEQ ID NO: 20, and TyrB comprises the amino acid sequence set forth in SEQ ID NO: 21.

7. The method of claim 5 , wherein AroG is replaced by a feedback inhibition resistant mutant AroG* encoded by a nucleic acid comprising the nucleotide sequence of SEQ ID NO: 27 and/or PheA is replaced by a feedback inhibition resistant mutant PheA* encoded by a nucleic acid comprising the nucleotide sequence of SEQ ID NO: 28.

8. The method of claim 5 , further comprising deletion or inactivation of crr and/or prephenate dehydrogenase (tyrA) genes.

9. The method of claim 1 , wherein the one-pot reaction system comprises the use of a tri-phasic medium comprising:
(a) an aqueous: organic solvent: solid resin medium; or
(b) an aqueous: organic solvent: functionalized nanoparticles medium.

10. A method for bioproduction of substituted or unsubstituted 2-phenylethanol or phenylethylamine by one or more recombinant bacterial or fungal cells genetically engineered to overexpress, relative to a wild type cell, at least one enzyme, which method comprises subjecting a starting material to a plurality of enzyme-catalyzed chemical transformations in an one-pot reaction system, wherein the starting material is styrene or substituted styrene, wherein the genetically engineered cells:
iii) overexpress styrene monooxygenase, styrene oxide isomerase, an aldehyde reductase and/or an alcohol dehydrogenase for generating substituted or unsubstituted 2-phenylethanol from the styrene or the substituted styrene, wherein the styrene monooxygenase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NOs: 1 and 2; the styrene oxide isomerase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO: 3; and the alcohol dehydrogenase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO: 5; or
iv) overexpress styrene monooxygenase, styrene oxide isomerase and a transaminase for generating substituted or unsubstituted phenylethylamine from the styrene or the substituted styrene, wherein the styrene monooxygenase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NOs: 1 and 2; the styrene oxide isomerase comprises an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO: 3; and the transaminase is ω-transaminase comprising an amino acid sequence having at least 90% identity to the sequence set forth in SEQ ID NO: 6.

Assignees

• Nat Univ Singapore

Inventors

• Li Zhi
• Wu Shuke
• Zhou Yi
• Lukito Benedict Ryan

Classifications

• C12P7/54

  Acetic acid (vinegar C12J) · CPC title

• C12P7/22

  aromatic · CPC title

• C12N9/0006

  acting on CH-OH groups as donors (1.1) · CPC title

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title

• C12Y207/01071

  Shikimate kinase (2.7.1.71) · CPC title