One-way separator for retaining and recirculating cells
US-9840691-B2 · Dec 12, 2017 · US
Adapter for cell-culture vessel
US11597901B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11597901-B2 |
| Application number | US-201716318109-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 19, 2017 |
| Priority date | Jul 29, 2016 |
| Publication date | Mar 7, 2023 |
| Grant date | Mar 7, 2023 |
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- Title
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Abstract
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Disclosed herein is a sterilizable adapter for a cell culture vessel comprising a body, an upper connection and/or a lid, at least one port, a ventilation passage, a lower internal connection suitable for connection to a cell culture vessel and a cell retention device and/or a cell separation device as well as a device comprising said sterilizable adapter and a method for cell cultivation using said sterilizable adapter.
First claim
Opening claim text (preview).
The invention claimed is: 1. A sterilizable adapter for a small-scale cell culture vessel comprising: a body, an upper connection and/or a lid, at least one port for medium addition, at least one second port for medium removal, a ventilation passage, a lower internal connection suitable for connection to the cell culture vessel and at least one cell retention device, wherein the cell culture vessel is a shake flask configured to removably receive the sterilizable adapter and the sterilizable adapter is a sterilizable adapter for a small-scale cell culture, with a cultivation volume of 1 ml to 500 ml, wherein the at least one cell retention device is a gravity cell settler and is inclined with an angle between 60° and 80° with respect to a horizontal line; and wherein the shake flask and adapter in use may be moved by constant orbital rotational movement at a rate of from 100 to 500 revolutions per minute. 2. The sterilizable adapter for a cell culture vessel according to claim 1 , further comprising at least one cell culture level sensor extending downwardly from the adapter toward a base of the shake flask such that the cell culture level sensor is about perpendicular to the horizontal line. 3. The sterilizable adapter for a cell culture vessel according to claim 1 , wherein the upper connection is an upper external screw thread and/or the lower internal connection suitable for connection to a cell culture vessel is a screw thread suitable for screwing in a screw thread. 4. The sterilizable adapter for a cell culture vessel according to claim 1 , wherein the at least one cell retention device is connected to at least one port. 5. The sterilizable adapter for a cell culture vessel according to claim 1 , wherein the at least one cell retention device is positioned in a liquid phase of the cell culture vessel. 6. The sterilizable adapter for a cell culture vessel according to claim 1 , wherein the at least one cell retention device is inclined with an angle between 70° and 75° with respect to a horizontal line. 7. The sterilizable adapter for a cell culture vessel according to claim 1 wherein the shake flask is an Erlenmeyer flask. 8. An article comprising the adapter of claim 1 removably mounted on a shake flask. 9. A shake flask comprising the sterilizable adapter of claim 1 .
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- What does patent US11597901B2 cover?
- Disclosed herein is a sterilizable adapter for a cell culture vessel comprising a body, an upper connection and/or a lid, at least one port, a ventilation passage, a lower internal connection suitable for connection to a cell culture vessel and a cell retention device and/or a cell separation device as well as a device comprising said sterilizable adapter and a method for cell cultivation using…
- Who is the assignee on this patent?
- Bayer Ag, Bayer Healthcare Llc
- What technology area does this patent fall under?
- Primary CPC classification C12M23/08. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 07 2023 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 5 related publications on this page (citations in our corpus or others sharing the same primary CPC).