Cleaning method of field-flow-fractionation apparatus

Patent metadata

Field	Value
Publication number	US-11592425-B2
Application number	US-202016776654-A
Country	US
Kind code	B2
Filing date	Jan 30, 2020
Priority date	Feb 4, 2019
Publication date	Feb 28, 2023
Grant date	Feb 28, 2023

Abstract

Provided is a field-flow-fractionation apparatus that is configured to supply a carrier fluid to a waste fluid chamber through a fluid supply flow path at a flow rate higher than a set flow rate of a flow rate adjusting part at a timing between an end of analysis of a sample and a start of analysis of a subsequent sample, thereby forming a flow of the carrier fluid from the waste fluid chamber to the separation channel. Accordingly, the sample adhering to a separation membrane is separated from the separation membrane and is discharged from the outlet port.

First claim

What is claimed is:

1. A cleaning method in a separation channel provided in a field-flow-fractionation apparatus,
the field-flow-fractionation apparatus including: a separation channel having one end provided with an inlet port and another end provided with an outlet port, and forming a space through which a carrier fluid flows; a separation membrane forming a wall surface defining the separation channel; a waste fluid chamber which forms a space through which the carrier fluid having passed through the separation membrane flows and has a discharge port through which the carrier fluid is discharged to the outside; and a flow rate adjusting part connected to the discharge port to adjust a flow rate of the carrier fluid to be discharged from the waste fluid chamber through the discharge port to a flow rate of a setting for the flow rate adjusting part,
the cleaning method comprising:
a cleaning step of, after an analysis of a sample is completed, supplying a carrier fluid to a portion between the waste fluid chamber and the flow rate adjusting part at a flow rate higher than the flow rate of the setting of the flow rate adjusting part without directly supplying the carrier fluid to the separation channel, wherein
in the cleaning step, a flow of a carrier fluid from the waste fluid chamber to the separation channel is formed and the carrier fluid which has flowed into the separation channel from the waste fluid chamber is discharged from the separation channel through the outlet port.

2. The cleaning method according to claim 1 , wherein
the setting for the flow rate adjusting part is set to zero in the cleaning step.

3. The cleaning method according to claim 1 , wherein
the cleaning step is performed in a state where a carrier fluid separate from the carrier fluid supplied to the portion between the waste fluid chamber and the flow rate adjusting part flows into and through the separation channel.

4. The cleaning method according to claim 1 , wherein the carrier fluid supplied to the portion between the waste fluid chamber and the flow rate adjusting part during the cleaning step enters the separation channel through the separation membrane.

5. A field-flow-fractionation apparatus comprising:
a separation channel having one end provided with an inlet port and the other end provided with an outlet port, and forming a space through which a carrier fluid supplied through the inlet port flows;
a separation membrane forming a wall surface defining the separation channel;
a waste fluid chamber forming a space through which the carrier fluid having passed through the separation membrane flows, and having a discharge port through which the carrier fluid having passed through the separation membrane is discharged to the outside;
a flow rate adjusting part connected to the discharge port to adjust a flow rate of the carrier fluid to be discharged from the waste fluid chamber through the discharge port to a flow rate of a setting for the flow rate adjusting part;
a fluid supply flow path connected to a portion between the waste fluid chamber and the flow rate adjusting part to supply a carrier fluid to the waste fluid chamber without first going through the separation channel;
a fluid supply part that supplies a carrier fluid to the waste fluid chamber through the fluid supply flow path; and
a control part configured to control the fluid supply part and the flow rate adjusting part to cause the field-flow-fractionation apparatus to perform a cleaning operation, wherein
the control part is configured, in the cleaning operation, to control the fluid supply part and the flow rate adjusting part to supply the carrier fluid to the waste fluid chamber through the fluid supply flow path at a flow rate higher than the flow rate of the setting for the flow rate adjusting part so that a flow of the carrier fluid from the waste fluid chamber to the separation channel is formed until analysis of a subsequent sample is started after analysis of a sample is completed, and wherein
the field-flow-fractionation apparatus is configured, in the cleaning operation, to cause the fluid which has flowed into the separation channel from the waste fluid chamber to be discharged from the separation channel through the outlet port.

6. The field-flow-fractionation apparatus according to claim 5 , wherein
the control part is configured to set the setting for the flow rate adjusting part to zero during the cleaning operation.

7. The field-flow-fractionation apparatus according to claim 5 , wherein
the control part is configured to perform the cleaning operation in a state where a carrier fluid is supplied into the separation channel through the inlet port.

8. The field-flow-fractionation apparatus according to claim 5 , further comprising:
a focus-flow fluid supply flow path connected to the separation channel at a carrier fluid supply position different from the inlet port to supply a carrier fluid to the separation channel so that a flow of a carrier fluid opposite to a flow of a carrier fluid from the inlet port is formed,
wherein the fluid supply part includes a fluid supply pump which supplies the carrier fluid and is connected, via a switching valve, to the focus-flow fluid supply flow path and the fluid supply flow path, and the fluid supply part is configured to select either the focus-flow fluid supply flow path or the fluid supply flow path as a channel for supplying the carrier fluid by switching the switching valve.

9. The field-flow-fractionation apparatus according to claim 5 , wherein
the fluid supply part includes a fluid supply pump which supplies the carrier fluid and is connected, via a switching valve, to an inlet flow path and the fluid supply flow path, the inlet flow path leads to the inlet port of the separation channel, the fluid supply part is configured to select either the inlet flow path or the fluid supply flow path as a channel for supplying the carrier fluid by switching the switching valve.

10. A cleaning method in a separation channel provided in a field-flow-fractionation apparatus,
the field-flow-fractionation apparatus including: a separation channel forming a space through which a carrier fluid flows; a separation membrane forming a wall surface defining the separation channel; a waste fluid chamber which forms a space through which the carrier fluid having passed through the separation membrane flows and has a discharge port through which the carrier fluid is discharged to the outside; and a flow rate adjusting part connected to the discharge port to adjust a flow rate of the carrier fluid to be discharged from the waste fluid chamber through the discharge port to a flow rate of a setting for the flow rate adjusting part,
the cleaning method comprising:
a cleaning step of, after an analysis of a sample is completed, supplying a cleaning fluid to a first portion between the waste fluid chamber and the flow rate adjusting part at a flow rate higher than the flow rate of the setting of the flow rate adjusting part without directly supplying the cleaning fluid to the separation channel, so that the cleaning fluid flows from the first portion into the waste fluid chamber.

11. The cleaning method according to claim 10 , wherein the cleaning fluid enters the waste fluid chamber, and subsequently enters the separation channel.

12. The cleaning method according to claim 11 , wherein the cleaning fluid supplied enters the separation channel through the separation membrane.

13. The cleaning method according to claim 12 , wherein
the cleaning step is performed in a state where a carrier fluid separate from the cleaning fluid flows into and through t

Assignees

• Shimadzu Corp

Inventors

• Oikawa Yukio
• Horiike Shigeyoshi

Classifications

• G01N30/0005 Primary

  Field flow fractionation · CPC title

• B01D63/005

  Microfluidic devices (Microfluidic devices comprising semi-permeable hollow fibre membranes B01D63/028; Microfluidic devices comprising semi-permeable flat membranes B01D63/088) · CPC title

• G01N2030/0025

  cross flow FFF · CPC title

• B01D65/02

  Membrane cleaning or sterilisation {; Membrane regeneration} · CPC title

• B01D63/088

  Microfluidic devices comprising semi-permeable flat membranes · CPC title