Nanobead containing biosensors and methods of production and use thereof

Patent metadata

Field	Value
Publication number	US-11585809-B2
Application number	US-201816486926-A
Country	US
Kind code	B2
Filing date	Feb 28, 2018
Priority date	Mar 3, 2017
Publication date	Feb 21, 2023
Grant date	Feb 21, 2023

Abstract

Multi-use biosensors are disclosed that include enzymes coupled to nanobeads; the multi-use biosensors are used to detect analytes in fluidic biological samples, and the biosensors also maintain their enzyme activity after many uses. Multi-sensor arrays are disclosed that include multiple biosensors. Also disclosed are methods of producing and using these devices.

First claim

What is claimed is:

1. A multi-use biosensor for detecting the presence and/or concentration of a target analyte in a fluidic biological sample, the multi-use biosensor comprising:
an electrode;
a plurality of nanobeads dispensed on the electrode, wherein each of the plurality of nanobeads has at least one enzyme coupled thereto via the plurality of nanobeads being coated with streptavidin and the at least one enzyme being conjugated to biotin, and wherein the enzyme interacts with the target analyte for detection of the target analyte; and
a semi-permeable membrane disposed on the plurality of the nanobeads, wherein the membrane immobilizes the plurality of nanobeads on the electrode, and wherein the membrane is permeable to the target analyte to be detected but substantially impermeable to the enzyme coupled to the nanobeads.

2. The multi-use biosensor of claim 1 , wherein the multi-use biosensor is a potentiometric analyte biosensor.

3. The multi-use biosensor of claim 1 , wherein each of the plurality of nanobeads has a diameter in a range of from about 100 nm to about 1 μm.

4. The multi-use biosensor of claim 1 , wherein the nanobeads are non-magnetic.

5. The multi-use biosensor of claim 1 , wherein the nanobeads comprise latex.

6. The multi-use biosensor of claim 1 , wherein the semi-permeable membrane is formed of a material selected from the group comprising polyurethane, silicone, poly(vinyl chloride), and combinations thereof.

7. The multi-use biosensor of claim 1 , wherein the multi-use biosensor is a multi-use blood urea nitrogen (BUN) biosensor, and wherein the at least one enzyme is urease.

8. The multi-use biosensor of claim 1 , wherein the biosensor has at least a 14 day use-life.

9. A multi-use biosensor array assembly, comprising:
a substrate;
a plurality of multi-use biosensors, each of the plurality of multi-use biosensors being a multi-use biosensor of claim 1 , wherein the enzymes of at least two of the plurality of multi-use biosensors are different, and wherein each of the plurality of multi-use biosensors are spatially positioned on at least one surface of the substrate.

10. The multi-use biosensor array assembly of claim 9 , wherein at least one of the plurality of multi-use biosensors is a multi-use blood urea nitrogen (BUN) biosensor, and wherein the at least one enzyme is urease.

11. A method for detecting the presence and/or concentration of a target analyte in a fluidic biological sample, the method comprising the steps of:
(a) inserting a fluidic biological sample into a blood gas, electrolyte, and/or metabolite instrument containing the multi-use biosensor of claim 1 ; and
(b) measuring the presence and/or concentration of the target analyte captured by the multi-use biosensor.

12. The method of claim 11 , wherein the multi-use biosensor is a multi-use blood urea nitrogen (BUN) biosensor.

13. The method of claim 11 , wherein the fluidic biological sample is selected from the group comprising blood, plasma, serum, urine, saliva, sputum, cerebrospinal fluid (CSF), skin, intestinal fluid, intraperitoneal fluid, cystic fluid, sweat, interstitial fluid, extracellular fluid, tears, mucus, bladder wash, semen, fecal, pleural fluid, nasopharyngeal fluid, and combinations thereof.

14. A method for detecting the presence and/or concentration of a plurality of target analytes in a fluidic biological sample, the method comprising the steps of:
(a) inserting a fluidic biological sample into a blood gas, electrolyte, and/or metabolite instrument containing the multi-use biosensor array assembly of claim 9 ; and
(b) measuring the presence and/or concentration of each of the plurality of target analytes captured by the individual multi-use biosensors of the array assembly.

15. The method of claim 14 , wherein at least one of the multi-use biosensors of the multi-use biosensor array assembly is a multi-use blood urea nitrogen (BUN) biosensor.

16. The method of claim 14 , wherein the fluidic biological sample is selected from the group comprising blood, plasma, serum, urine, saliva, sputum, cerebrospinal fluid (CSF), skin, intestinal fluid, intraperitoneal fluid, cystic fluid, sweat, interstitial fluid, extracellular fluid, tears, mucus, bladder wash, semen, fecal, pleural fluid, nasopharyngeal fluid, and combinations thereof.

17. A method of producing a multi-use analyte biosensor, the method comprising the steps of:
(a) coupling at least one enzyme to at least one nanobead via the at least one nanobead being coated with streptavidin and the at least one enzyme being conjugated to biotin;
(b) depositing a plurality of nanobeads having at least one enzyme coupled thereto on an electrode; and
(c) disposing a semi-permeable membrane on the enzyme crosslinked nanobeads and electrode, wherein the membrane immobilizes the plurality of nanobeads on the electrode, and wherein the membrane is permeable to the analyte to be detected but substantially impermeable to the enzyme coupled to the nanobeads.

18. The method of claim 17 , wherein the multi-use analyte biosensor is a potentiometric analyte biosensor.

19. The method of claim 17 , wherein each of the plurality of nanobeads has a diameter in a range of from about 100 nm to about 1 μm.

20. The method of claim 17 , wherein the nanobeads are non-magnetic.

21. The method of claim 17 , wherein the nanobeads comprise latex.

22. The method of claim 17 , wherein the semi-permeable membrane is formed of a material selected from the group comprising polyurethane, silicone, poly(vinyl chloride), and combinations thereof.

23. The method of claim 17 , wherein the biosensor is a multi-use blood urea nitrogen (BUN) biosensor, and wherein the at least one enzyme is urease.

24. The method of claim 17 , wherein the biosensor has at least a 14 day use-life.

25. The method of claim 17 , further comprising the step of:
(d) determining an activity of the enzyme prior to step (b).

26. A method of producing a multi-use biosensor array assembly, the method comprising the step of:
forming a plurality of multi-use biosensors on at least one surface of a substrate, wherein each of the plurality of multi-use biosensors is formed by the method of claim 17 , wherein the enzymes of at least two of the plurality of multi-use biosensors are different, and wherein each of the plurality of multi-use biosensors are spatially positioned on the at least one surface of the substrate.

27. The method of claim 26 , wherein at least one of the multi-use biosensors is a multi-use blood urea nitrogen (BUN) biosensor, and wherein the at least one enzyme is urease.

Assignees

• Siemens Healthcare Diagnostics Inc

Inventors

• Wilson Michael S

Classifications

• G01N27/3278 Primary

  involving nanosized elements, e.g. nanogaps or nanoparticles (nanopores G01N33/48721; magnetic beads G01N27/745) · CPC title

• G01N33/54346

  Nanoparticles · CPC title

• G01N27/333

  Ion-selective electrodes or membranes (glass electrodes G01N27/36) · CPC title

• C12Q1/58 Primary

  involving urea or urease · CPC title

• G01N33/54386 Primary

  Analytical elements · CPC title