Potentiating agents for protecting plants from fungal infections

Patent metadata

Field	Value
Publication number	US-11566260-B2
Application number	US-201916564501-A
Country	US
Kind code	B2
Filing date	Sep 9, 2019
Priority date	Jul 16, 2012
Publication date	Jan 31, 2023
Grant date	Jan 31, 2023

Abstract

Disclosed is a method for preventing, controlling or treating a fungal infection on a plant. The method includes applying to such plant organ a non-fungicidal amount or a potentiating amount of a composition including a potentiating agent of a plant defense molecule, in association with a phytopharmaceutical vehicle.

First claim

The invention claimed is:

1. A method for controlling or treating a fungal infection by a phytopathogenic fungal strain on a plant organ, said method comprising the steps of:
i) in vitro determining the non-fungicidal concentration of at least one potentiating agent of a plant defense molecule selected from the group consisting of chelerythrine, sanguinarine; and Cl − , HSO 4 − , I − , HCO 3 − salts thereof, against the phytopathogenic fungal strain;
said determination being carried out by comparing the growth of the phytopathogenic fungal strain cultures in contact with increasing concentrations of said at least one potentiating agent of a plant defense molecule, with the growth of a control culture of the phytopathogenic fungal strain, in the absence of said at least one potentiating agent of a plant defense molecule; the last concentration of the increasing concentrations of the at least one potentiating agent of a plant defense molecule resulting in the same fungal culture growth as the control culture being retained as the non-fungicidal concentration of said at least one potentiating agent of a plant defense molecule; then

ii) applying on the plant organ a composition comprising said at least one potentiating agent of a plant defense molecule in the determined non-fungicidal concentration, such that the amount applied is a potentiating amount but is not an amount that is fungicidal per se, in association with a phytopharmaceutical vehicle.

2. The method according to claim 1 , wherein said in vitro determination of the non-fungicidal amount of at least one potentiating agent of a plant defense molecule is carried-out by spectrophotometry or nephelometry.

3. The method according to claim 1 , wherein said phytopathogenic fungal infection is an infection by a phytopathogenic fungus selected from the group consisting of the genera Alternaria, Sclerotinia and Venturia.

4. The method according to claim 1 , wherein said fungal infection is an infection by a phytopathogenic fungus selected from the group consisting of Alternaria brassicicola, Alternaria dauci and Ventuna inaequalis.

5. The method according to claim 1 , wherein the plant is selected from the group consisting of plants of the Brassicacae, Apiaceae, Vitaceae and Rosaceae families.

6. The method according to claim 1 , wherein the plant is selected from the group consisting of Brassica carinata, Brassica juncea, Brassica oleracea, Brassica napus, Brassica nigra and Brassica rapa.

7. The method according to claim 1 , wherein the plant is selected from the group consisting of Brassica oleracea, Daucus carota subsp. Sativa and Malus domestica.

8. The method according to claim 1 , wherein the plant organ, the phytopathogenic fungus and the non-fungicidal concentration of the at least one potentiating agent of a plant defense molecule in the applied composition is selected from the following combinations:
a. said plant organ belonging to a plant of the Brassicaceae family, said phytopathogenic fungus being a strain of the Alternaria genus, and said non-fungicidal concentration of the at least one potentiating agent of a plant defense molecule in the applied composition being from 1 to 25 μM; or
b. said plant organ belonging to a plant of the Apiaceae family, said phytopathogenic fungus being a strain of the Alternaria genus, and said non-fungicidal concentration of the at least one potentiating agent of a plant defense molecule in the applied composition being from 1 to 10 μM; or
c. said plant organ belonging to a plant of the Rosaceae family, said phytopathogenic fungus being a strain of the Venturia genus, and said non-fungicidal concentration of the at least one potentiating agent of a plant defense molecule in the applied composition being from 1 to 5 μm.

9. The method according to claim 1 , wherein the plant organ, the phytopathogenic fungus and the non-fungicidal concentration of the at least one potentiating agent of a plant defense molecule in the applied composition is selected from the following combinations:
a. said plant organ belonging to a plant of the Brassicaceae family plant, said phytopathogenic fungus being Alternaria brassicicola , and said non-fungicidal concentration of the at least one potentiating agent of a plant defense molecule in the applied composition being from 1 to 25 μM; or
b. said plant organ belonging to the Daucus carota plant, said phytopathogenic fungus being Alternaria dauci , and said non-fungicidal concentration of the at least one potentiating agent of a plant defense molecule in the applied composition being from 1 to 10 μM; or
c. said plant organ belonging to the Malus domestica plant, said phytopathogenic fungus being Venturia inaequalis , and said non-fungicidal concentration of the at least one potentiating agent of a plant defense molecule in the applied composition being from 1 to 5 μm.

10. The method according to claim 1 , wherein said plant organ belongs to Brassica oleracea plant, said phytopathogenic fungus being Alternaria brassicicola , and said non-fungicidal concentration of the at least one potentiating agent of a plant defense molecule in the applied composition being from 1 to 25 μM.

11. The method according to claim 1 , wherein the composition further comprises a plant defense molecule selected from the group consisting of brassinin, camalexin, resveratrol, 3,5-dihydroxybiphenyl, aucuparin and 6-methoxymellein.

12. The method according to claim 1 , wherein the composition further comprises an agent for stimulating the production of a plant defense molecule.

13. The method according to claim 1 , wherein the composition further comprises at least one agent for stimulating the production of a plant defense molecule; said agent being selected from the group consisting of acibenzolar-S-methyl, chitosan, laminarin, Reynoutria Sachalinensis extract, calcium prohexadione, harpine, yeast wall extracts, oligogalacturonides and calcium phosphite.

14. The method according to claim 1 , wherein the composition further comprises an insecticide and/or a herbicide.

Assignees

• Univ Angers

Inventors

• Simoneau Philippe
• Guillemette Thomas
• Richomme Pascal
• Helesbeux Jean-Jacques

Classifications

• A01N65/00

  Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof (containing compounds of determined constitution A01N27/00 - A01N59/00) · CPC title

• A01N63/30

  Microbial fungi; Substances produced thereby or obtained therefrom · CPC title

• C12N15/8282 Primary

  for fungal resistance · CPC title

• A01N65/08

  Magnoliopsida [dicotyledons] · CPC title

• A01N43/90 Primary

  having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system · CPC title