Dendritic cell generator

Patent metadata

Field	Value
Publication number	US-11566224-B2
Application number	US-202016905615-A
Country	US
Kind code	B2
Filing date	Jun 18, 2020
Priority date	Jun 29, 2015
Publication date	Jan 31, 2023
Grant date	Jan 31, 2023

Abstract

Devices, systems, and methods can be used for the automated production of dendritic cells (DC) from dendritic cell progenitors, such as monocytes obtained from peripheral blood. The invention makes it possible to obtain sufficient quantities of a subject's own DC for use in preparing and characterizing vaccines, for activating and characterizing the activation state of the subject's immune response, and to aid in preventing and/or treating cancer or infectious disease.

First claim

What is claimed is:

1. A method for dendritic cell generation, the method comprising:
providing a cell culture chamber comprising a fluidic channel and an inlet;
introducing a liquid sample containing monocytes into the fluidic channel via the inlet; and
flowing a fluid through the fluidic channel such that a flow rate of the fluid through the fluidic channel is less than a sedimentation rate of the monocytes and dendritic cells within the fluidic channel, thereby maintaining the monocytes and dendritic cells within the fluidic channel without use of filters.

2. The method of claim 1 , wherein the cell culture chamber further comprises an outlet, and a cell binding surface, and wherein the chamber is configured such that the fluidic channel is elevated in a manner that the inlet is below the outlet.

3. The method of claim 2 , wherein the cell binding surface has a surface area from about 2 cm2 to about 100 cm2, and the cell culture chamber has a height from about 0.1 mm to about 2 mm.

4. The method of claim 2 , wherein the cell binding surface comprises a polystyrene surface.

5. The method of claim 4 , wherein the cell binding surface forms a bottom of the fluidic channel.

6. The method of claim 5 , wherein other surfaces of the fluidic channel are comprised of a second material to which cells do not bind.

7. The method of claim 1 , wherein the cell culture chamber is configured for operation within a cell culture incubator.

8. The method of claim 1 , wherein the fluid comprises a dendritic cell differentiation medium.

9. The method of claim 8 , wherein the dendritic cell differentiation medium comprises at least one compound selected from the group consisting of IL4, GM-CSF, IL1β, IL6, TNFα, PGE2, and a combination thereof.

10. The method of claim 9 , wherein the dendritic cell differentiation medium is flowed continuously.

11. The method of claim 2 , wherein the cell culture chamber is supported by a mounting area of a device comprising a base such that the fluidic channel is at an acute angle with respect to the base.

12. The method of claim 2 , wherein the monocytes and dendritic cells are maintained within the fluidic channel without the use of filters on account of their masses.

13. The method of claim 2 , wherein the cell culture chamber is configured for observation underneath a microscope.

14. The method of claim 2 , wherein the cell biding surface comprises a monocyte-biding substrate.

15. The method of claim 14 , wherein the monocyte-binding substrate comprises a CD14 antibody.

16. A method for dendritic cell generation, the method comprising:
introducing a liquid sample containing monocytes into a fluidic channel of a cell culture chamber via an inlet of the cell culture chamber; and
flowing a fluid through the fluidic channel such that a flow rate of the fluid through the fluidic channel is less than a sedimentation rate of the monocytes and dendritic cells within the fluidic channel.

17. The method of claim 16 , wherein the monocytes and dendritic cells are maintained within the fluidic channel without use of filters.

18. A method for dendritic cell generation, the method comprising:
causing a fluid to flow through a fluidic channel of a cell culture chamber comprising monocytes introduced into the fluidic channel via an inlet of the cell culture chamber, the fluid being caused to flow at a flow rate of less than a sedimentation rate of the monocytes and dendritic cells within the fluidic channel.

19. The method of claim 18 , wherein the fluid flows through the fluidic channel unfiltered.

20. The method of claim 18 , further comprising elevating an outlet of the cell culture chamber relative to an inlet of the cell culture chamber.

Assignees

• Univ Northeastern

Inventors

• Murthy Shashi K
• Collier Bradley B

Classifications

• C07K14/545

  IL-1 · CPC title

• C12M23/16

  Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093) · CPC title

• C12M41/14

  Incubators; Climatic chambers (per se B01L1/00) · CPC title

• C07K14/525

  Tumour necrosis factor [TNF] · CPC title

• A61K39/00

  Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53) · CPC title