Acoustic wave based particle agglomeration

Patent metadata

Field	Value
Publication number	US-11560557-B2
Application number	US-201716461753-A
Country	US
Kind code	B2
Filing date	Nov 17, 2017
Priority date	Nov 18, 2016
Publication date	Jan 24, 2023
Grant date	Jan 24, 2023

Abstract

Articles of manufacture, including an apparatus for acoustic wave based agglomeration, are provided. The apparatus may include a well and an acoustic wave device. The well may be configured to hold a suspension that includes a plurality of particles. The acoustic wave device may be configured to generate a plurality of acoustic waves. The plurality of acoustic waves inducing acoustic streaming within the suspension. The acoustic streaming agitating the suspension to form an agglomerate comprising at least a portion of the plurality of particles. Methods for acoustic wave based agglomeration are also provided.

First claim

What is claimed is:

1. An apparatus, comprising:
a well including a suspension comprising a plurality of particles; and
an acoustic wave device configured to generate a plurality of acoustic waves, wherein the plurality of acoustic waves induce acoustic streaming and formation of a vortex within the suspension contained by the well, wherein the acoustic streaming including the vortex agitates the suspension to cause formation of an agglomerate within the well, wherein the agglomerate comprises at least a portion of the plurality of particles,
wherein an orientation of the acoustic wave device is configured such that the plurality of acoustic waves enters a bottom portion of the well, wherein the well comprises a top portion through which the suspension is inserted into the well and the bottom portion contains the suspension along with a side portion of the well,
wherein the acoustic wave device comprises a piezoelectric material configured to convert electric energy into the plurality of acoustic waves, wherein the acoustic wave device comprising the piezoelectric material is oriented via one or more ramps to control the angle of incidence of the plurality of acoustic waves entering the bottom portion of the well and to control a distance between the acoustic streaming in the suspension and a center of the well, wherein the acoustic wave device comprising the piezoelectric material is coupled to the well via a couplant material, wherein the well is at least partially submerged in the couplant material, and
wherein the orientation of the acoustic wave device is configured such that the plurality of acoustic waves enters, based on the orientation provided by the one or more ramps, via the bottom portion of the well.

2. The apparatus of claim 1 , wherein the agglomerate comprises a 3-dimensional formation comprising at least the portion of the plurality of particles.

3. The apparatus of claim 1 , wherein the plurality of particles comprise cells.

4. The apparatus of claim 1 , wherein the suspension comprises a mixture of the plurality of particles and one or more fluids.

5. The apparatus of claim 1 , wherein the piezoelectric material comprises a monocrystalline and/or a polycrystalline.

6. The apparatus of claim 1 , wherein 50 milliwatts to 5.0 watts of electric power is applied to the piezoelectric material in order to cause the acoustic wave device to generate the plurality of acoustic waves.

7. The apparatus of claim 1 , wherein the acoustic wave device is configured to generate the plurality of acoustic waves in one or more intermittent bursts.

8. The apparatus of claim 7 , wherein a length of the one or more intermittent bursts of acoustic waves is between 1 second and 100 seconds.

9. The apparatus of claim 7 , wherein each of the one or more intermittent bursts of acoustic waves triggers a corresponding cycle of the acoustic streaming.

10. The apparatus of claim 9 , wherein the acoustic wave device is configured to expose the suspension to between 1 cycle to 1000 cycles of the acoustic streaming, and wherein each cycle of the acoustic streaming is between 0.1 seconds per minute to 15 seconds per minute.

11. The apparatus of claim 1 , wherein the acoustic wave device is configured to operate in accordance with a duty ratio, and wherein the duty ratio corresponds to a proportion of total elapsed time during which the acoustic wave device is generating the plurality of acoustic waves.

12. The apparatus of claim 11 , wherein the duty ratio is between 10% and 50%.

13. The apparatus of claim 1 , wherein the apparatus further comprises the couplant material configured to transmit the plurality of acoustic waves from the acoustic wave device to the well.

14. The apparatus of claim 1 , wherein the acoustic wave device is oriented such that the plurality of acoustic waves enters the bottom portion of the well at between an 5° angle of incidence and an 55° angle of incidence.

15. The apparatus of claim 1 , wherein the acoustic wave device is oriented such that the acoustic streaming is induced at between to of a distance between ½ to ¾ of a distance between a center of the well and an edge of the well.

Assignees

• Univ California
• Friend James
• Takemura Kenjiro
• Kurashina Yuta

Inventors

• Friend James
• Takemura Kenjiro
• Kurashina Yuta

Classifications

• B06B1/0215

  for generating pulses, e.g. bursts of oscillations, envelopes · CPC title

• B06B1/0603

  using a piezoelectric bender, e.g. bimorph · CPC title

• C12M47/02

  Separating microorganisms from the culture medium; Concentration of biomass (separating microorganisms from their culture media C12N1/02) · CPC title

• B01L2200/0647

  Handling flowable solids, e.g. microscopic beads, cells, particles · CPC title

• C12M33/08

  by vibration · CPC title