Elite event EE-GM5 and methods and kits for identifying such event in biological samples

Patent metadata

Field	Value
Publication number	US-11555202-B2
Application number	US-201716471776-A
Country	US
Kind code	B2
Filing date	Dec 22, 2017
Priority date	Dec 22, 2016
Publication date	Jan 17, 2023
Grant date	Jan 17, 2023

Abstract

The invention provides specific transgenic soybean plants, plant material and seeds, characterized in that these products harbor a specific nematode resistance and herbicide tolerance transformation event at a specific location in the soybean genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.

First claim

The invention claimed is:

1. A nucleic acid molecule comprising
(a) a nucleotide sequence with at least 99% sequence identity to the nucleotide sequence of SEQ ID No. 5, 6, 24 or 25, or to the complement of SEQ ID No. 5, 6, 24 or 25;
(b) the nucleotide sequence of SEQ ID No. 1, 2, 3, 4, 5, 6, 24, or 25 or the complement of SEQ ID No. 1, 2, 3, 4, 5, 6, 24, or 25; or
(c) the nucleotide sequence of SEQ ID No. 1 or 3 and the nucleotide sequence of SEQ ID No. 2 or 4, or the complement of SEQ ID No. 1 or 3 and the complement of SEQ ID No. 2 or 4.

2. The nucleic acid molecule of claim 1 , further comprising:
(a) the nucleotide sequence of SEQ ID No. 7 and 9, or the complement of SEQ ID No. 7 and 9;
(b) the nucleotide sequence of SEQ ID No. 11 from nucleotide position 188 to nucleotide position 7368; or
(c) a nucleotide sequence having at least 98%, or least 99%, or at least 99.5% or at least 99.9% sequence identity to the nucleotide sequence of (a) or (b).

3. The nucleic acid molecule of claim 2 , wherein the molecule comprises the nucleotide sequence of SEQ ID No. 5 or 24 and SEQ ID No. 6 or 25, or the complement of SEQ ID No. 5 or 24 and SEQ ID No. 6 or 25.

4. A nucleic acid molecule obtainable from the seed deposited at the ATCC under accession number PTA-123625, wherein said nucleic acid molecule comprises the nucleotide sequence of any one of SEQ ID No. 1, 3, or 5 and the nucleotide sequence of any one of SEQ ID No. 2, 4, or 6.

5. Soybean genomic DNA comprising the nucleic acid molecule of claim 1 .

6. A nucleic acid molecule comprising the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941, or a nucleotide sequence having at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941.

7. A nucleic acid molecule that specifically characterizes soybean elite transformation event EE-GM5, wherein it comprises (i) the nucleotide sequence of any one of SEQ ID No. 1, 3, or 5, and/or (ii) the nucleotide sequence of SEQ ID No. 2, 4, or 6.

8. A nucleic acid molecule comprising (a) a nucleotide sequence with at least 98% sequence identity to SEQ ID No. 7 or 9; or (b) a nucleotide sequence with at least 95% sequence identity to SEQ ID No. 11.

9. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises a nucleotide sequence with at least 99% sequence identity to the nucleotide sequence of SEQ ID No. 5, 6, 24 or 25, or to the complement of SEQ ID No. 5, 6, 24 or 25.

10. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 1 or the complement of SEQ ID No. 1.

11. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 2 or the complement of SEQ ID No. 2.

12. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 3 or the complement of SEQ ID No. 3.

13. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 4 or the complement of SEQ ID No. 4.

14. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 5 or the complement of SEQ ID No. 5.

15. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 6 or the complement of SEQ ID No. 6.

16. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 24 or the complement of SEQ ID No. 24.

17. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 25 or the complement of SEQ ID No. 25.

18. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 1 or 3 and the nucleotide sequence of SEQ ID No. 2 or 4, or the complement of SEQ ID No. 1 or 3 and the complement of SEQ ID No. 2 or 4.

19. The nucleic acid molecule of claim 6 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941.

20. The nucleic acid molecule of claim 6 , wherein the nucleic acid molecule comprises a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941.

21. The nucleic acid molecule of claim 6 , wherein the nucleic acid molecule comprises a nucleotide sequence having at least 96% sequence identity to the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941.

22. The nucleic acid molecule of claim 6 , wherein the nucleic acid molecule comprises a nucleotide sequence having at least 97% sequence identity to the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941.

23. The nucleic acid molecule of claim 6 , wherein the nucleic acid molecule comprises a nucleotide sequence having at least 98% sequence identity to the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941.

24. The nucleic acid molecule of claim 6 , wherein the nucleic acid molecule comprises a nucleotide sequence having at least 99% sequence identity to the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941.

25. The nucleic acid molecule of claim 8 , wherein the nucleic acid molecule comprises a nucleotide sequence with at least 98% sequence identity to SEQ ID No. 7 or 9.

26. The nucleic acid molecule of claim 8 , wherein the nucleic acid molecule comprises a nucleotide sequence with at least 95% sequence identity to SEQ ID No. 11.

Assignees

• BASF Agricultural Solutions Seed US LLC

Inventors

• Moser Hal
• Mccarville Michael
• Buyse Maxim
• Slabbinck Filip
• Beilinson Vadim
• Kleven Thomas W
• Daum Julia
• Aartsen Wendy
• Habex Veerle

Classifications

• C12N15/8279

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• C12N15/8274 Primary

  for herbicide resistance · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• A01N35/06

  containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals · CPC title

• C12Q2600/158

  Expression markers · CPC title