Determining a frequency for TTFields treatment based on an electrical characteristic of targeted cancer cells

Patent metadata

Field	Value
Publication number	US-11554262-B2
Application number	US-202016800737-A
Country	US
Kind code	B2
Filing date	Feb 25, 2020
Priority date	Feb 26, 2019
Publication date	Jan 17, 2023
Grant date	Jan 17, 2023

Abstract

Cancer treatment using TTFields (Tumor Treating Fields) can be customized to each individual subject by obtaining cancer cells from the subject, determining an electrical characteristic (e.g., dielectrophoretic forces, cell membrane capacitance, etc.) of the cancer cells, determining a frequency for the TTFields based on the determined electrical characteristic, and treating the cancer by applying TTFields to the subject at the determined frequency. In addition, cancer treatment can be planned for each individual subject by obtaining cancer cells from the subject, determining an electrical characteristic of the cancer cells, predicting whether TTFields would be effective to treat the cancer based on the determined electrical characteristic, and treating the subject by applying TTFields if the prediction indicates that TTFields would be effective.

First claim

What is claimed is:

1. A method of treating cancer in a subject, the method comprising:
extracting at least one cancer cell from the subject's body;
determining an electrical characteristic of the extracted at least one cancer cell;
determining a frequency of alternating electric fields to apply to the subject in order to treat the cancer based on the determined electrical characteristic; and
treating the cancer by applying an alternating electric field to the subject at the determined frequency of alternating electric fields.

2. The method of claim 1 , wherein the determining of the electrical characteristic comprises measuring dielectrophoretic forces of the extracted at least one cancer cell at each of a plurality of frequencies below 35 kHz.

3. The method of claim 1 , wherein the determining of the frequency comprises choosing a frequency that is effective in treating reference cancer cells that have a known electrical characteristic, wherein the known electrical characteristic matches the determined electrical characteristic.

4. The method of claim 1 , wherein the determining of the electrical characteristic comprises determining cell membrane capacitance.

5. A method of treating cancer in a subject, the method comprising:
extracting at least one cancer cell from the subject's body;
measuring dielectrophoretic force of the extracted at least one cancer cell;
determining a frequency of alternating electric fields to apply to the subject in order to treat the cancer based on the measured dielectrophoretic force; and
treating the cancer by applying an alternating electric field to the subject at the determined frequency of alternating electric fields.

6. The method of claim 5 , wherein the cell membrane capacitance of the at least one cancer cell can be determined from the measured dielectrophoretic force.

7. A method of treating cancer in a subject, the method comprising:
extracting at least one cancer cell from the subject's body;
determining an electrical characteristic of the extracted at least one cancer cell;
predicting whether application of alternating electric fields to the subject would be effective to treat the cancer based on the determined electrical characteristic; and
treating the cancer by applying an alternating electric field to the subject if the predicting indicates that application of alternating electric fields to the subject would be effective to treat the cancer.

8. The method of claim 7 , wherein the determining of the electrical characteristic comprises measuring dielectrophoretic forces of the extracted at least one cancer cell at each of a plurality of frequencies below 35 kHz.

9. The method of claim 7 , wherein the predicting is based on whether reference cancer cells that have an electrical characteristic that matches the determined electrical characteristic are susceptible to treatment using alternating electric fields.

10. The method of claim 7 , wherein the determining of the electrical characteristic comprises determining cell membrane capacitance.

11. A method of treating cancer in a subject the method comprising:
extracting at least one cancer cell from the subject's body;
measuring dielectrophoretic force of the extracted at least one cancer cell;
predicting whether application of alternating electric fields to the subject would be effective to treat the cancer based on the measured dielectrophoretic force; and
treating the cancer by applying an alternating electric field to the subject if the predicting indicates that application of alternating electric fields to the subject would be effective to treat the cancer.

12. The method of claim 11 , wherein the cell membrane capacitance of the at least one cancer cell can be determined from the measured dielectrophoretic force.

Assignees

• Novocure Gmbh

Inventors

• Giladi Moshe
• Zeevi Einav
• Wenger Cornelia
• Bomzon Zeev

Classifications

• A61N1/40

  Applying electric fields by inductive or capacitive coupling (microwave apparatus A61N5/00); {Applying radio-frequency signals} · CPC title

• A61N1/36002 Primary

  Cancer treatment, e.g. tumour · CPC title

• G01N27/447

  using electrophoresis · CPC title

• G01N33/48728

  Investigating individual cells, e.g. by patch clamp, voltage clamp (investigating individual particles in general G01N15/10) · CPC title

• G01N27/221

  by investigating the dielectric properties (using microwaves G01N22/00; measuring loss factors or dielectric constants per se G01R27/26) · CPC title