Nanocarriers for the delivery of active ingredients

Patent metadata

Field	Value
Publication number	US-11554190-B2
Application number	US-201816640040-A
Country	US
Kind code	B2
Filing date	Aug 17, 2018
Priority date	Aug 18, 2017
Publication date	Jan 17, 2023
Grant date	Jan 17, 2023

Abstract

Various embodiments of the present invention relate to, among other things, a nano carrier platform for generating enhanced engineered water nanostructures (iEWNS) encapsulating and delivering reactive oxygen species (ROS) and, in some instances, other active ingredients, methods for inactivating at least one of viruses, bacteria, bacterial spores, and fungi on a substrate by applying iEWNS to the substrate.

First claim

What is claimed is:

1. A method for inactivating at least one of viruses, bacteria, bacterial spores, fungi, and natural flora present on a substrate comprising:
applying enhanced engineered water nanostructures (iEWNS) for a targeted and precise delivery to the substrate to inactivate the at least one of viruses, bacteria, bacterial spores, fungi and natural flora;
the iEWNS comprising (i) reactive oxygen species (ROS), (ii) at least one active ingredient in addition to the ROS, and (iii) a surface charge, wherein the at least one active ingredient is present separate and in addition to any at least one active ingredient that is generated when the iEWNS are generated.

2. The method of claim 1 , wherein the substrate is selected from the group consisting of a wound, skin, foodstuffs, surfaces and air.

3. The method of claim 1 , wherein the active ingredient is an antimicrobial active ingredient.

4. The method of claim 3 , wherein the antimicrobial active ingredient is at least one of hydrogen peroxide, one or more inorganic antimicrobials, one or more organic acids and salts thereof, one or more chemical antimicrobials, one or more ovo antimicrobials, one or more lacto antimicrobials, one or more bacto antimicrobials, and one or more phyto antimicrobials, inorganic colloids, emulsions or combinations thereof.

5. The method of claim 1 , wherein the bacteria are at least one of gram-positive and gram-negative bacteria.

6. The method of claim 1 , wherein the bacteria comprise mycobacteria.

7. The method of claim 1 , wherein the inactivation comprises reducing the number of colony forming units (cfu) on the substrate by about ≤1 to about 8 log 10 compared to control.

8. The method of claim 1 , wherein the inactivation comprises reducing the number of colony forming units (cfu) on the substrate at a rate of from about 0.05 log 10 /min to about 1.01 log 10 /min, compared to control.

9. The method of claim 1 , wherein the ROS concentration in the rEWNS is from about 20 μM to about 60 μM after at a total sampling time of from about 5 minutes to about 15 minutes.

10. The method of claim 1 , wherein the applying delivers a total dose of the at least one active ingredient of from about 1 pg to about 1 μg in about one hour or less.

11. A system, comprising:
a fluid source configured to contain an aqueous composition comprising at least one active ingredient;
an electrode;
a conduit, coupled to the fluid source and forming an aperture, wherein at least one of the conduit and the electrode are adjustably positionable with respect to one another to set a predetermined adjustable distance from the aperture to the electrode; and
a variable voltage source, electrically coupled between the conduit and the electrode, configured to impart an electric potential difference between the conduit and the electrode;
wherein fluid from the fluid source that passes through conduit and the aperture is changed to enhanced engineered water nanostructures (iEWNS) upon coming into proximity of the electrode based on the predetermined distance and the electric potential, wherein the iEWNS comprise the at least one active ingredient encapsulated therein, and wherein the at least one active ingredient is present separate and in addition to any at least one active ingredient that is generated when the iEWNS are generated.

12. The system of claim 11 , wherein the at least one active ingredient is at least one of reactive oxygen species (ROS) and at least one active ingredient in addition to the ROS.

13. The system of claim 11 , wherein the active ingredient is an antimicrobial active ingredient.

14. The system of claim 13 , wherein the antimicrobial active ingredient is at least one of hydrogen peroxide, one or more inorganic antimicrobials, one or more organic acids and salts thereof, one or more chemical antimicrobials, one or more ovo antimicrobials, one or more lacto antimicrobials, one or more bacto antimicrobials, one or more phyto antimicrobials, inorganic colloids, emulsions and combinations thereof.

15. A method, comprising:
coupling a conduit to a fluid source configured to contain at least one active ingredient, the conduit forming an aperture,
adjustably positioning the conduit with respect to an electrode to set a predetermined adjustable distance from the aperture to the electrode; and
electrically coupling a variable voltage source between the conduit and the electrode, the variable voltage source configured to impart an electric potential difference between the conduit and the electrode;
wherein fluid from the fluid source that passes through conduit and the aperture is changed to enhanced electrolyzed water engineered water nanostructures (iEWNS) comprising at least one active ingredient encapsulated therein upon coming into proximity of the electrode based on the predetermined distance and the electric potential and the at least one active ingredient is present separate and in addition to any at least one active ingredient that is generated when the iEWNS are generated.

16. The method of claim 15 , wherein the active ingredient is an antimicrobial active ingredient.

17. The method of claim 16 , wherein the antimicrobial active ingredient is at least one of hydrogen peroxide, one or more organic acids and salts thereof, one or more chemical antimicrobials, one or more ovo antimicrobials, one or more lacto antimicrobials, one or more bacto antimicrobials, one or more phyto antimicrobials, inorganic colloids, emulsions and combinations thereof.

18. A method for increasing shelf life of produce by increasing the firmness of the produce without altering sensory characteristics and pH, comprising treating the produce with enhanced electrolyzed water engineered water nanostructures (iEWNS) comprising at least one active ingredient encapsulated therein, wherein the at least one active ingredient is present separate and in addition to any at least one active ingredient that is generated when the iEWNS are generated.

19. A method for at least one of reducing the formation of biofilms produced by bacteria and reducing the strength of the biofilms produced by bacteria comprising:
treating a substrate comprising bacteria with enhanced electrolyzed water engineered water nanostructures (iEWNS) comprising at least one active ingredient encapsulated therein, wherein the at least one active ingredient is present separate and in addition to any at least one active ingredient that is generated when the iEWNS are generated.

20. The method of claim 19 , wherein the formation of biofilm produced is reduced by at least 50%.

Assignees

• Harvard College

Inventors

• Demokritou Philip
• Vaze Nachiket Dattatray
• Pyrgiotakis Georgios
• Eleftheriadou Mary

Classifications

• A61L2/035

  Electrolysis · CPC title

• C02F1/005

  Systems or processes based on supernatural or anthroposophic principles, cosmic or terrestrial radiation, geomancy or rhabdomancy · CPC title

• C02F2305/023

  Reactive oxygen species, singlet oxygen, OH radical · CPC title

• A61L2/186 Primary

  Peroxide solutions · CPC title

• A61L9/14

  using sprayed or atomised substances {including air-liquid contact processes} · CPC title