Phytates for use as a biomolecules delivery or adsorption system

Patent metadata

Field	Value
Publication number	US-11554128-B2
Application number	US-201917044768-A
Country	US
Kind code	B2
Filing date	Apr 10, 2019
Priority date	Apr 16, 2018
Publication date	Jan 17, 2023
Grant date	Jan 17, 2023

Abstract

The present invention relates to mineral micro-particles comprising phytate (inositol hexaphosphate, IP6). More particularly, the invention provides salts of phytic acid with multivalent metal ions such as Ca2+ and Mg2+ for use in biomolecules delivery or adsorption systems, methods for their production and uses thereof, such as for use as a vaccine adjuvant.

First claim

The invention claimed is:

1. Mineral microparticles comprising one or more salts of phytic acid (IP6), the IP6 salts comprising organic polycations and_divalent alkaline earth metal ions M 2+ selected from Ca 2+ , Mg 2+ or mixtures thereof,
wherein the microparticles have a nominal size of at least 0.5 μm and at most 2 μm, and wherein the stoichiometric ratio of phytic acid to the divalent alkaline earth metal ions is 1:6 to 1:8, and
wherein an antigen is adsorbed on a surface of at least a portion of the microparticles.

2. The mineral microparticles according to claim 1 , wherein calcium phytate is present and has a nominal zeta-potential when measured at pH 7.0 in distilled water of at least −34 mV.

3. The mineral microparticles according to claim 1 , wherein the mineral microparticles are present in a biomolecules delivery system and the biomolecules delivery system is a vaccine adjuvant.

4. A vaccine adjuvant composition comprising mineral micro particles,
wherein the mineral microparticles comprise one of more salts of phytic acid (IP6), the IP6 salts comprising organic polycations and_divalent alkaline earth metal ions M 2+ selected from Ca 2+ , Mg 2+ or mixtures thereof,
wherein the vaccine adjuvant composition is combined with an antigen to form a vaccine composition,
wherein the microparticles have a size of at least 0.5 μm and at most 2 μm, and
wherein an antigen is adsorbed on a surface of at least a portion of the microparticles.

5. The adjuvant composition according to claim 4 wherein the mineral microparticles have a size of between 0.5 to 1 μm.

6. A antigen delivery or adsorption system comprising the mineral microparticles according to claim 1 .

7. The antigen delivery or adsorption system according to claim 6 , wherein the antigen delivery system is a vaccine adjuvant.

8. The mineral microparticles according to claim 1 , wherein the organic polycation is selected from the group consisting of (poly)lysine and poly-D-glucosamine.

Assignees

• Croda Int Plc

Inventors

• Duroux Laurent
• Lindblad Erik

Classifications

• A61K2039/55505

  Inorganic adjuvants · CPC title

• A61K39/39

  characterised by the immunostimulating additives, e.g. chemical adjuvants · CPC title

• A61K31/6615 Primary

  Compounds having two or more esterified phosphorus acid groups, e.g. inositol triphosphate, phytic acid · CPC title

• A61K9/145

  with organic compounds · CPC title

• A61K33/06

  Aluminium, calcium or magnesium; Compounds thereof {, e.g. clay} · CPC title