Adipose tissue centrifuge and method of use

Patent metadata

Field	Value
Publication number	US-11549094-B2
Application number	US-202016893464-A
Country	US
Kind code	B2
Filing date	Jun 5, 2020
Priority date	Jan 31, 2014
Publication date	Jan 10, 2023
Grant date	Jan 10, 2023

Abstract

A centrifuge device is provided for the sizing and separation of constituents of a biologic mixture, e.g., adipose tissue. The device provides for the mechanical breaking down of the fibrous structure in the tissue by centrifugation causing the tissue to pass through a mesh element, or a sizing helix, or an extrusion element, whereupon the material is reduced to a slurry. This processed material may then be separated by centrifugation into its constituents, in order to harvest the fraction containing the multipotent cells. These multipotent cells may be utilized for various medical procedures to stimulate healing and tissue regeneration.

First claim

What is claimed is:

1. A method for separating a component from a tissue material comprising the steps of:
a. introducing a tissue material into a chamber of a centrifuge, said chamber having a central longitudinal axis about which said chamber is arranged to be rotated and said chamber comprising a sidewall with a tapered inner surface, an annular screen having an inner surface located at a first radial distance from said central longitudinal axis, and a trap, the trap being located in said chamber adjacent said inner surface of said sidewall at a second radial distance from said central longitudinal axis, wherein the second radial distance is greater than the first radial distance,
b. rotating said chamber about the central longitudinal axis to pass at least a substantial portion of the tissue material through said annular screen, thereby morselizing the substantial portion of the tissue material and causing the tissue material to stratify in said chamber into at least two constituent layers as a function of differing specific gravities of the constituent layers, and
c. collecting at least a portion of the highest specific gravity constituent layer in the trap.

2. The method of claim 1 , wherein the tissue material is adipose tissue and the highest specific gravity component comprises stem cells.

3. The method of claim 1 , wherein the trap is an annular trap.

4. The method of claim 1 , wherein the trap is angled relative to the central longitudinal axis.

5. The method of claim 3 , wherein the trap is angled relative to the central longitudinal axis.

6. The method of claim 1 , wherein the centrifuge further comprises a first port located in the trap.

7. The method of claim 6 , wherein the first port is located in the trap at the largest outside diameter of the trap.

8. The method of claim 1 , wherein the trap comprises an angled passageway having an innermost portion through which the highest specific gravity constituent layer enters the trap.

9. The method of claim 1 , wherein the chamber comprises a base and the annular screen projects away from the base, wherein the chamber further comprises a wedge present in the base, the wedge comprising an outer surface that is angled relative to the central longitudinal axis, and wherein an outer surface of the wedge and the inner surface of the sidewall define the trap.

10. The method of claim 9 , wherein the wedge comprises an angled inner surface, and wherein the chamber comprises a dished area in the base, the dished area at least partially defined by the angled inner surface of the wedge.

11. The method of claim 10 , wherein upon cessation of the rotation of the chamber, one or more constituent layers settle into the dished area of the chamber and do not enter the trap.

12. The method of claim 10 , wherein the centrifuge further comprises a second port located within the dished area.

13. The method of claim 6 , further comprising the step of opening the first port during the rotation of the chamber, thereby ejecting at least a portion of the highest specific gravity constituent layer from the trap.

14. The method of claim 1 , further comprising the step of removing at least a portion of the highest specific gravity constituent layer from the trap via a needle or cannula, wherein the portion of the tissue material so recovered comprises stem cells.

15. The method of claim 6 , wherein the centrifuge comprises an outer housing containing the chamber and the outer housing comprises an access opening for accessing material collected in the trap via the first port.

16. The method of claim 15 , further comprising the step of extracting stem cells from the trap via a syringe by inserting the syringe into the access opening and through the first port.

17. The method of claim 1 , wherein the chamber further comprises a secondary screen, the secondary screen being located at a third radial distance from the central longitudinal axis, wherein the third radial distance is greater than the first radial distance and less than the second radial distance, wherein the secondary annular screen is configured to capture fibrous material while allowing non-fibrous material and liquid to pass through.

18. The method of claim 1 , wherein the centrifuge further comprises a roller arranged to roll around the inner surface of the annular screen and urge at least a portion of the tissue material through the annular screen and away from the central longitudinal axis and towards the sidewall.

19. The method of claim 1 , wherein the centrifuge further comprises a roller arranged to roll against and around the inner surface of the annular screen and urge at least a portion of the biologic mixture through the annular screen and away from the central longitudinal axis and towards the sidewall.

20. A method for separating a cellular component from a tissue material comprising the steps of:
a. introducing a tissue material into a chamber of a centrifuge, said chamber having a central longitudinal axis about which said chamber is arranged to be rotated and said chamber comprising a sidewall with an angled inner surface, a trap in fluid communication with the interior of the chamber and located adjacent said inner surface of said sidewall at the greatest distance from the longitudinal axis within the chamber, and a screen located between the trap and the central longitudinal axis,
b. rotating said chamber about the central longitudinal axis,
c. causing at least a substantial portion of the tissue material to pass through said screen and stratify into at least two constituent layers as a function of differing specific gravities of the constituent layers, and
d. directing at least a portion of the highest specific gravity constituent layer to the trap via contact with the sidewall, wherein the highest specific gravity constituent comprises a cellular component.

Assignees

• Dsm Ip Assets Bv

Inventors

• Nash John E
• Fisher William T
• Bradica Gino

Classifications

• C12N2527/00

  Culture process characterised by the use of mechanical forces, e.g. strain, vibration · CPC title

• C12N5/0653

  Adipocytes; Adipose tissue · CPC title

• C12M47/04 Primary

  Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04) · CPC title

• B04B3/00 Primary

  Centrifuges with rotary bowls in which solid particles or bodies become separated by centrifugal force and simultaneous sifting or filtering · CPC title

• C12N5/0068

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title