Air to liquid micro-fluidic chamber

Patent metadata

Field	Value
Publication number	US-11548001-B1
Application number	US-201916379890-A
Country	US
Kind code	B1
Filing date	Apr 10, 2019
Priority date	Apr 10, 2018
Publication date	Jan 10, 2023
Grant date	Jan 10, 2023

Abstract

A system, apparatus, and method include a pump to deliver vapor including airborne contaminants including organic compounds including a target analyte; a collector to transfer the airborne contaminants by autonomous liquid extraction into a mobile organic liquid phase; a micro-fluidic chamber including immobilized biorecognition elements that bind to analytes delivered from the mobile organic liquid phase; a mechanism to introduce the mobile organic liquid phase to a buffer containing a plurality of substrates causing a series of biochemical reactions that create a change corresponding to a concentration of the target analyte; and a detector to perform real-time analysis that correlates to a concentration of the organic compounds to determine a presence of the target analyte.

First claim

What is claimed is:

1. A system comprising:
a gas-to-liquid collector configured to transfer a vapor by autonomous liquid extraction to a mobile organic liquid phase, the vapor comprising an organo-phosphate;
a micro-fluidic chamber, configured to receive the mobile organic liquid phase from the gas-to-liquid collector, the micro-fluidic chamber comprising enzymes therein that are configured to react with a reagent to yield a hydrogen ion and an electron, the enzymes comprising acetylcholine esterase, cyclooxygenase, horseradish peroxidase, or combinations thereof;
a substrate configured to receive the mobile organic liquid phase with the hydrogen ion and the electron from the micro-fluidic chamber, the substrate configured to chemically react with the hydrogen ion and the electron to yield a fluorophore; and
a detector configured to detect and to perform real-time analysis of fluorescence of the fluorophore,
wherein presence of the organo-phosphate inhibits the enzymes within the micro-fluidic chamber, thereby reducing a yield of the fluorophore, and wherein a decrease in fluorescence corresponds to an increase in organo-phosphate.

2. The system of claim 1 , wherein the gas-to-liquid collector comprises a tube comprising silica gel coated with a xerogel to collect the target analyte.

3. The system of claim 1 , wherein the gas-to-liquid collector comprises a microelectromechanical systems (MEMS) device to collect the organo-phosphate.

4. The system of claim 1 , comprising:
a reservoir configured to store the mobile organic liquid phase; and
a valve configured to control delivery of the mobile organic liquid phase from the reservoir to the gas-to-liquid collector.

5. The system of claim 1 , further comprising:
an alarm that is triggered upon detection of the organo-phosphate at a concentration that is greater than a predetermined level.

6. The system of claim 1 , wherein the mobile organic liquid phase comprises a non-polar solvent.

7. The system of claim 6 , wherein the non-polar solvent comprises hexane.

8. The system of claim 1 , wherein the micro-fluidic chamber includes an inner surface and the enzymes are immobilized to the inner surface of micro-fluidic chamber.

9. The system of claim 8 , wherein the enzymes are immobilized by a cross-linker.

Assignees

• Us Gov Air Force
• Us Air Force

Inventors

• Rubenstein Mitchell
• Adkins Douglas
• Lewis Patrick
• Rogers John
• Kim Sungbong
• Kim Steve
• Brothers Michael

Classifications

• B01L3/502738 Primary

  characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746) · CPC title

• C12M23/16

  Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093) · CPC title

• F16K99/0001 Primary

  Microvalves (microdevices B81B1/00; manufacture or treatment of devices or systems in or on a substrate B81C1/00; microfluidic structures B01L3/5027; micropumps F04B19/006) · CPC title

• F16K2099/0084

  Chemistry or biology, e.g. "lab-on-a-chip" technology · CPC title

• C07F9/11

  with hydroxyalkyl compounds without further substituents on alkyl · CPC title