Method of producing extract and extraction residue of biological material, extract, and extraction residue

Patent metadata

Field	Value
Publication number	US-11547953-B2
Application number	US-201916980728-A
Country	US
Kind code	B2
Filing date	Mar 12, 2019
Priority date	Mar 16, 2018
Publication date	Jan 10, 2023
Grant date	Jan 10, 2023

Abstract

A method of producing an extract of an animal-derived or plant-derived biological material includes: extracting a component in the biological material using liquefied dimethyl ether for the biological material to obtain a liquefied dimethyl ether solution including the component; separating the solution from the biological material; and volatilizing or separating the liquefied dimethyl ether from the solution.

First claim

The invention claimed is:

1. A method of producing an extraction residue of a biological material, the method comprising:
feeding the biological material into an extraction tank having a filter disposed on an upstream side and a filter disposed on a downstream side, wherein the upstream side is connected to a storage tank containing liquefied dimethyl ether by a first conduit having a first valve and the downstream side is connected directly to a separation tank by a second conduit having a second valve, wherein the first valve and the second valve are closed during the feeding of the biological material into the extraction tank, wherein the biological material is an animal-derived material;
opening the first valve to allow the liquefied dimethyl ether in the storage tank to be fed into the extraction tank through the first conduit and subsequently closing the first valve;
extracting a component in the biological material using liquefied dimethyl ether for dissolving the biological material to obtain a liquefied dimethyl ether solution including the component;
opening the first valve and the second valve, thereby providing fresh liquefied dimethyl ether from the storage tank by the first conduit and feeding the liquefied dimethyl ether solution including the component only to the separation tank by the second conduit;
volatilizing or otherwise separating dimethyl ether from the liquefied dimethyl ether solution in the separation tank to obtain an extraction residue;
decomposing a nucleic acid component included in the extraction residue using a nuclease; and
removing the nucleic acid component from the extraction residue.

2. The method of producing an extraction residue of a biological material according to claim 1 , wherein
the liquefied dimethyl ether is admixed with an auxiliary solvent in an amount equal to or less than a saturation amount of the liquefied dimethyl ether, wherein the amount of the auxiliary solvent is equal to or less than 7% by mass relative to the liquefied dimethyl ether.

3. The method of producing an extraction residue of a biological material according to claim 2 , wherein
the auxiliary solvent is water or alcohol.

Assignees

• Ricoh Co Ltd

Inventors

• Torii Shogo
• Shinohara Satoshi
• Suzuki Shogo

Classifications

• B01D11/0288 Primary

  Applications, solvents · CPC title

• B01D11/028

  Flow sheets · CPC title

• B01D37/00

  Processes of filtration (processes specially adapted for filtering gases B01D46/00) · CPC title

• B01D11/0257

  using mixing mechanisms, e.g. stirrers, jets (B01D11/0242 takes precedence) · CPC title

• A61K35/44

  Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells · CPC title