Graded porous scaffolds as immunomodulatory wound patches

Patent metadata

Field	Value
Publication number	US-11547776-B2
Application number	US-201816608381-A
Country	US
Kind code	B2
Filing date	Apr 26, 2018
Priority date	Apr 26, 2017
Publication date	Jan 10, 2023
Grant date	Jan 10, 2023

Abstract

The present invention provides porous biomimetic scaffolds and methods for making the same. The scaffolds have graded pore sizes for enhanced cell penetration. The scaffolds are useful for wound regeneration by facilitating cell penetration into the scaffold interior and due to their inherent immunomodulatory effects. The scaffolds have tissue modeling specification by mimicking the inherent stratified structure of certain tissues.

First claim

What is claimed is:

1. A scaffold comprising:
a material having a gradient of pores comprising plant protein fibers having a first surface, a second surface, and a thickness therebetween;
wherein the average pore size at the first surface is smaller than the average pore size at the second surface;
wherein the average pore size gradually increases through the material thickness from the first surface to the second surface;
wherein the pores of the first surface prohibit the passage of cells; and
wherein the second surface is configured to allow cell penetration into the thickness of the material up to the first surface.

2. The scaffold of claim 1 , wherein the plant protein is selected from the group consisting of: soy protein isolate, wheat gluten, corn zein, and pea protein.

3. The scaffold of claim 1 , wherein the fibers have a diameter between 0.5 μm and 5 μm.

4. The scaffold of claim 1 , wherein the scaffold has a thickness between 500 μm and 2000 μm.

5. The scaffold of claim 1 , wherein the average pore size of the first surface is between 1 μm and 20 μm in diameter.

6. The scaffold of claim 1 , wherein the average pore size of the second surface is between 10 μm and 200 μm.

7. The scaffold of claim 1 , wherein the gradual increase of the average pore size is linear.

8. The scaffold of claim 1 , wherein the gradual increase of the average pore size is nonlinear.

9. The scaffold of claim 1 , wherein the scaffold is capable of supporting cell growth.

10. The scaffold of claim 1 , further comprising at least one cell.

11. The scaffold of claim 1 , further comprising at least one material selected from the group consisting of: fibronectin, laminin, collagen, glycoprotein, thrombospondin, elastin, fibrillin, mucopolysaccharide, glycolipid, heparin sulfate, chondroitin sulfate, keratin sulfate, glycosaminoglycan, hyaluronic acid, proteoglycan, vitronectin, poly-D-lysine, and polysaccharide.

12. The scaffold of claim 1 , further comprising at least one material selected from the group consisting of poly(epsilon-caprolactone) (PCL), poly(lactic acid) (PLA), poly(glycolic acid) (PGA), copolymers poly (lactide-co-glycolide) (PLGA), polyaniline, and poly(ethylene oxide) (PEO).

Assignees

• Temple University—Of the Commonwealth System of Higher Education
• Univ Temple

Inventors

• Timnak Azadeh
• Lelkes Peter I
• Har-El Yah-El H

Classifications

• A61L15/32

  Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin {(A61L15/225 takes precedence)} · CPC title

• A61L27/60 Primary

  Materials for use in artificial skin · CPC title

• D10B2509/022

  Wound dressings · CPC title

• A61L27/227 Primary

  Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24 · CPC title

• A61L27/56 Primary

  Porous materials, {e.g. foams or sponges} · CPC title