Matrix-assisted laser desorption/ionization mass spectrometry method

Patent metadata

Field	Value
Publication number	US-11545349-B2
Application number	US-201716347040-A
Country	US
Kind code	B2
Filing date	Oct 30, 2017
Priority date	Nov 3, 2016
Publication date	Jan 3, 2023
Grant date	Jan 3, 2023

Abstract

The present invention relates to a matrix-assisted laser desorption ionization mass spectrometry method and, specifically, a mass spectrometry method according to the present invention comprises the steps of: acquiring a mass spectrum of an analyte by performing matrix-assisted laser desorption ionization of the analyte, wherein a detection spectrum, which is the mass spectrum of the analyte, is acquired using each of two or more matrixes different from one another; and removing, from each detection spectrum, a peak of a corresponding matrix to obtain a matrix-removed spectrum, and then acquiring a corrected mass spectrum of the analyte on the basis of a matrix-removed spectrum for each of different matrixes.

First claim

The invention claimed is:

1. A matrix-assisted laser desorption ionization (MALDI) mass spectrometry method comprising:
performing matrix-assisted laser desorption ionization on an analyte to obtain a mass spectrum, wherein a detection spectrum which is a mass spectrum of the analyte is obtained using each of two or more different matrices used for a same mass range, wherein each of two or more detection spectrums consists of peaks from the same mass ranges; and removing a peak of a corresponding matrix from each detection spectrum to obtain a matrix-removed spectrum and subsequently obtaining a corrected mass spectrum of the analyte on a basis of the matrix-removed spectrum of each of the different matrices,
wherein the corrected mass spectrum is calculated by merging peaks commonly present in the two or more matrix-removed spectrums and a peak present only in one matrix-removed spectrum.

2. The MALDI mass spectrometry method of claim 1 , comprising step a) of irradiating a first sample including the analyte and a first matrix with a laser to obtain a first detection spectrum and irradiating a second sample including the analyte and a second matrix with the laser to obtain a second detection spectrum;
step b) of removing a peak of the first matrix from the first detection spectrum to obtain a first matrix-removed spectrum and removing a peak of the second matrix from the second detection spectrum to obtain a second matrix-removed spectrum; and step c) of obtaining the corrected mass spectrum of the analyte on the basis of the first matrix-removed spectrum and the second matrix-removed spectrum.

3. The MALDI mass spectrometry method of claim 2 , wherein step c) includes: merging a common peak as a peak commonly present in the first matrix-removed spectrum and the second matrix-removed spectrum and a complementary peak as a peak present only in one of the first matrix-removed spectrum and the second matrix-removed spectrum.

4. The MALDI mass spectrometry method of claim 3 , further comprising:
correcting an intensity of the complementary peak using an intensity ratio between the common peak of the first matrix-removed spectrum and the common peak of the second matrix-removed spectrum, in the merging.

5. The MALDI mass spectrometry method of claim 3 , further comprising:
calculating an average intensity for each m/z of the common peak to obtain a common peak spectrum, in the merging; and multiplying a ratio, which is obtained by dividing an intensity of one peak on the common peak spectrum by an intensity in the same m/z as the one peak on the matrix-removed spectrum to which the merged complementary peak belongs, by an intensity of the complementary peak to correct the intensity of the complementary peak.

6. The MALDI mass spectrometry method of claim 2 , wherein step b) further includes: standardizing each of the first detection spectrum and the second detection spectrum, before removal of the matrix peak, wherein the standardization step is performed by dividing an intensity of each peak present in each detection spectrum by a total intensity obtained by accumulating the intensity of each peak present in each detection spectrum.

7. The MALDI mass spectrometry method of claim 2 , further comprising:
irradiating the laser to each of a first reference sample not containing the analyte and containing the first matrix and a second reference sample not containing the analyte and containing the second matrix to obtain a mass spectrum originating from each matrix, before step a).

8. The MALDI mass spectrometry method of claim 2 , wherein a smallest m/z difference in mass (m/z) among mass (m/z) differences between peaks belonging to matrices different from each other in the peaks of the first matrix and the peaks of the second matrix is 1 or greater.

9. The MALDI mass spectrometry method of claim 1 , wherein the different matrices are two or more substances selected from among α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), 2-(4-hydroxyphenylazo)-benzoic acid (HABA), 2-mercaptobenzo-thiazole (MBT), and 3-Hydroxypicolinic acid (3-HPA).

10. The MALDI mass spectrometry method of claim 1 , wherein the analyte includes a compound of 1000 daltons or less.

11. The MALDI mass spectrometry method of claim 1 , wherein the MALDI mass spectrometry uses a time-of-flight (TOF) mass spectrometer (MS), an ion trap (IT) MS, a Fourier transform ion cyclotron resonance (FT-ICR) MS, a quadrupole MS, or an orbitrap MS.

Assignees

• Bioneer Corp

Inventors

• Kim Taeman
• Kim Do Hoon
• Ahn Jong Rok
• Park Han-Oh

Classifications

• H01J49/16

  using surface ionisation, e.g. field-, thermionic- or photo-emission · CPC title

• H01J49/0418 Primary

  for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates · CPC title

• H01J49/164

  Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI] (sample holders H01J49/0418) · CPC title

• H01J49/40

  Time-of-flight spectrometers (H01J49/36 takes precedence) · CPC title

• H01J49/161

  using photoionisation, e.g. by laser · CPC title